Concentration of homovanillic acid and 5-hydroxyindoleacetic acid in the ventricular cerebrospinal fluid of patients with obstructive hydrocephalus.

Kmieciak-Kołada, K; Majchrzak, H; Herman, Zbigniew S.

doi:10.1136/jnnp.42.1.78

Cited by 2 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The levels of 5-HIAA were comparable with previous findings as was the obvious concentration gradient between lumbar and ventricular level, appearing still higher than reported, possibly due to hydrocephalic patients: high pressure is known to be associated with increased 5-HIAA level (15).…”

Section: Discussionsupporting

confidence: 89%

“…Anticonvulsive medications may cause difficulties in interpretation of the results (7,19,25,26). Determination of trace amines, especially 5-HT, and their metabolites involves many difficulties and there are evidently gradients between different parts of the ventricular and cisternal system (5,7,15,27,29). However, these measurements are worthwhile because of the CSF concentrations may reflect amine metabolism in the CNS and analyses may be valuable both in diagnostics and in therapeutics (1, 30).…”

Section: Discussionmentioning

confidence: 99%

“…However, these measurements are worthwhile because of the CSF concentrations may reflect amine metabolism in the CNS and analyses may be valuable both in diagnostics and in therapeutics (1, 30). The number of diseases in which alterations in CSF amine metabolites have been reported is large (3, 4, 15,19,30,31,32,33), but large volumes of CSF needed in most methods have been an obstacle to determinations. By the present method the serotonin pathway is measured using only 2CL100 p1 of CSF and the results indicate a good correlation with those obtained by the fluorometric methods for TRP and 5-HIAA determinations but not for 5-HT, evidently due to the extraction procedure needed in the fluorometric method (12).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Indole levels in human lumbar and ventricular cerebrospinal fluid and the effect of L-tryptophan administration

Koskiniemi

Laakso

Kuurne

et al. 2009

Acta Neurologica Scandinavica

View full text Add to dashboard Cite

Levels of tryptophan (TRP), 5-hydroxytryptophan (5-HTP), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIM) in human lumbar and ventricular cerebrospinal fluid (CSF) were measured by reversed phase liquid chromatography (HPLC) with electrochemical detection. The levels of TRP ranged from 1593 to 4865 nmol/l in ventricular (VF) and from 1257 to 2557 nmol/l in lumbar CSF. The level of 5-HTP vaned from 1.1 to 68.9 nmol/l in VF and from 5.3 to 10.8 nmol/l in lumbar CSF; no previous reports of 5-HTP levels in CSF exist. The serotonin level was 1.9-27.3 nmol/l in VF and 5.7-12.0 nmol/l in lumbar CSF. The levels of 5-HIAA were considerbaly higher in VF than in lumbar fluid with respective means of 498 f 52.4 nmol/l and 112 k 15.6 nmol/l (P < 0.001). An oral dose of 2 g L-tryptophan significantly increased all indole levels except that of 5-HT, both in patients with progressive myoclonus epilepsy and in controls.Measurement of cerebrospinal fluid (CSF) concentrations of biochemically active compounds or their metabolites gives indirect information on cerebral metabolism (1). Serotonin (5-hydroxytryptamine, 5-HT) appears to play a role in alterations in the seizure threshold, in affective illnesses and in vascular accidents (2, 3, 4). The CSF concentration of serotonin seems a reasonable index of its content in the central nervous system ( C N S ) (5). Tryptophan availability and its concentration in the CSF is related to 5-hydroxyindoleacetic acid (5-HIAA) concentrations in the CSF (6, 7), and 5-HIAA reflects CNS 5-HT turnover (8). Therefore measurement of 5-HT Address M. Koskiniemi, M.D.

show abstract

Section: Discussionsupporting

confidence: 89%