5-HIAA and HVA in the Coma Cerebri, Hydrocephalus and Tumor Cerebri

Salihović, Mirsada; Sofić, E.

doi:10.1007/978-981-10-4166-2_61

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…Various analytical methods, such as spectrophotometric determination [2] and high-performance liquid chromatography (HPLC) [3], are commonly employed to detect homovanillic acid (HVA). However, despite their precision and sensitivity, these techniques necessitate expensive equipment and are impractical for on-site analysis.…”

Section: Introductionmentioning

confidence: 99%

Paper-based Colorimetric Assay: Rapid Detection of HVA in Urine Samples

Bhalchim,

Undale,

Shewale

2024

Preprint

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Diagnosing and monitoring of Parkinson's disease, schizophrenia, and certain mood disorders involves detecting homovanillic acid an essential aspect excreted through urine. In this study, we explored novel approaches for detecting homovanillic acid (HVA) in urine samples, aiming for cost-effective and on-site analysis methods. Traditional techniques like spectrophotometric determination and HPLC, while sensitive and selective, are often impractical due to their expense and lack of portability. Instead, we investigated the utility of paper-based analytical devices (PADs), which have gained popularity for various analytical applications including chemical element identification and environmental contamination assessment. We utilized different types of Whatmann filter papers (No. 1, No. 2, No. 4) and chromatography paper as substrates for the PADs, and employed combination of potassium ferrocyanide and ferric chloride reagent for HVA detection. Our experiments revealed positive results, indicating the efficacy of this approach. Optimization experiments identified 30 minutes to 3 hours as the optimal immersion times, with a subsequent 30-minute drying period at room temperature. Notably, variations in performance were observed among the different filter papers, with Whatmann CF6 paper exhibiting significant differences compared to the others in terms of HVA detection efficiency.

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Section: Introductionmentioning

confidence: 99%

Paper-based Colorimetric Assay: Rapid Detection of HVA in Urine Samples

Bhalchim,

Undale,

Shewale

2024

Preprint

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“…Indolic acids can be detected in serum [16][17][18] or plasma [13,19] and cerebrospinal fluid (CSF) [20,21] samples using GC-MS [16][17][18][19] and high-performance liquid chromatography-mass spectrometry (HPLC-MS) [16] methods. LLE [13,[17][18][19] and solid-phase extraction [22] are applied as sample preparation techniques.…”

Section: Introductionmentioning

confidence: 99%

Determination of Tryptophan Metabolites in Serum and Cerebrospinal Fluid Samples Using Microextraction by Packed Sorbent, Silylation and GC–MS Detection

et al. 2020

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Indole-containing acids—tryptophan metabolites—found in serum and cerebrospinal fluid (CSF) samples of patients with diseases of the central nervous system (CNS) were determined with the use of microextraction by packed sorbent (MEPS) followed by silylation and gas chromatography–mass spectrometry (GC–MS) analysis. MEPS with the following silylation led to the reproducible formation of derivatives with an unsubstituted hydrogen ion in the indole ring, the chromatographic peaks of which are symmetric and can be used for GC–MS analysis without additional derivatization. The recoveries of analytes at the limit of quantitation (LOQ) levels were 40–80% for pooled CSF and 40–60% for serum. The limit of detection (LOD) and LOQ values were 0.2–0.4 and 0.4–0.5 µM, respectively, for both CSF and serum. The precision (the reproducibility, RSD) value of less than 20% and the accuracy (the relative error, RE) value of less than ±20% at the LOQ concentrations meet the Food and Drug Administration (FDA) recommendations. Linear correlations for all analytes were determined over a potentially clinically significant range of concentrations (0.4–10 µM for serum, R2 ≥ 0.9942, and 0.4–7 µM for CSF, R2 ≥ 0.9949). Moreover, MEPS significantly reduced the matrix effect of serum compared to liquid–liquid extraction (LLE), which was revealed in the example of reducing the amount of cholesterol and its relative compounds.

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Hydrocephalus: 5-HIAA and HVA in the Cerebrospinal Fluid

Salihović

Sofić

2021

IFMBE Proceedings

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5-HIAA and HVA in the Coma Cerebri, Hydrocephalus and Tumor Cerebri

Cited by 3 publications

References 9 publications

Paper-based Colorimetric Assay: Rapid Detection of HVA in Urine Samples

Paper-based Colorimetric Assay: Rapid Detection of HVA in Urine Samples

Determination of Tryptophan Metabolites in Serum and Cerebrospinal Fluid Samples Using Microextraction by Packed Sorbent, Silylation and GC–MS Detection

Hydrocephalus: 5-HIAA and HVA in the Cerebrospinal Fluid

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