Concentration of circulating endothelial progenitor cells (EPC) in normal pregnancy and in pregnant women with diabetes and hypertension

Buemi, Michele; Allegra, Alessandro; D’Anna, Rosario; Coppolino, Giuseppe; Crascì, Eleonora; Giordano, D.; Loddo, Saverio; Cucinotta, Mariapaola; Musolino, Caterina; Teti, Diana

doi:10.1016/j.ajog.2006.08.032

Cited by 52 publications

(53 citation statements)

References 35 publications

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“…Hyperglycemia induces dysfunction and apoptosis of EPCs (5); this may impair the development or maturation of the utero-placental circulation, causing maladaptive responses during diabetic pregnancies. Indeed, EPCs have been shown to be dysregulated in pregnant women with diabetes (4).…”

mentioning

confidence: 99%

“…Recent studies in human pregnancies suggest that a mother's EPCs are involved in the physiologic vascular remodeling of systemic and utero-placental circulation (3,4). Hyperglycemia induces dysfunction and apoptosis of EPCs (5); this may impair the development or maturation of the utero-placental circulation, causing maladaptive responses during diabetic pregnancies.…”

mentioning

confidence: 99%

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Maternal Insulin Therapy Increases Fetal Endothelial Progenitor Cells During Diabetic Pregnancy

Fadini

Baesso²,

Agostini³

et al. 2008

Diabetes Care

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confidence: 99%

mentioning

confidence: 99%

Maternal Insulin Therapy Increases Fetal Endothelial Progenitor Cells During Diabetic Pregnancy

Fadini

Baesso²,

Agostini³

et al. 2008

Diabetes Care

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“…Buemi et al (6) suggested that the number of CD133 + and VEGF2 + endothelial progenitor cells in the maternal circulation increased with increasing gestational age, and that women with gestational hypertension had higher levels of the latter cells when compared with healthy pregnant women.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, Sugawara et al (5) demonstrated that the number of EPCs increases in the second and third trimesters. Buemi et al (6) observed that the number of isolated EPCs gradually increases every trimester.…”

Section: Introductionmentioning

confidence: 99%

Genomic and proteomic investigation of preeclampsia

Şahın

Günel

Benian

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of this study was to use proteomic and transcriptomic approaches to examine differences in protein and gene expression in maternal plasma between normal and preeclamptic pregnancies. Preeclampsia and control groups were compared with respect to the expression of CD34 and CD133 genes by quantitative polymerase chain reaction (qPCR) and heat shock protein (Hsp)27 and 70 by western blotting in blood samples from the pregnant women. Blood samples were obtained at gestational week 12-14 from 65 healthy pregnant women. Fetal DNA was isolated from the maternal blood and CD34 and CD133 were amplified by qPCR. Western blot analysis was used to examine the expression levels of Hsp27 and Hsp70 proteins. The analysis of CD133 by qPCR was unsuccessful in 7 women as the levels of fetal DNA were in the collected maternal blood samples were insufficient. Measurements of CD34 and CD133 were performed in 57 and 50 women, respectively. Preeclampsia developed in 6 (10.5%) of 57 women. qPCR results of 8 healthy pregnant women were used for the calibration of CD34 and CD133 levels, and the results for the remaining women were compared with the calibration values. CD34 expression was decreased in 30 (52.6%) and increased in 27 (47.4%) of 57 women. CD133 expression was decreased in 14 (28%) and increased in 36 (72%) of 50 women. CD34 expression was increased in 2 (33%) and 25 (49%) and decreased in 4 (66%) and 25 (51%) women with and without preeclampsia, respectively (P=0.467). CD133 expression was increased in 4 (66%) and 32 (72%); and decreased in 2 (33%) and 12 (28%) women with and without preeclampsia, respectively (P=0.756). Western blotting showed that the expression of Hsp27 and Hsp70 in the maternal serum of the preeclampsia group was significantly higher than that in the normal pregnancy group. CD34 and CD133 were found to be inadequate for use in the prediction of preeclampsia. However, it is noteworthy that CD133 levels were increased in 66 and 72% of women with and without preeclampsia, respectively. Hsps are expressed under various pathological conditions. These results suggest that conditions of oxidative stress increased the Hsp27 and Hsp70 protein levels.

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“…29 The cells were analyzed for the expression of surface antigens with direct three-color flow-cytometry analysis using fluorescein isothiocyanate-, phycoerythrin-and allophycocyanine-conjugated monoclonal antibodies (mAbs), as reported elsewhere. 30 Staining and analysis were performed using the International Society of Hematotherapy and Graft Engineering guidelines. All peripheral blood samples were collected and stored in 0.34 M K3EDTA anticoagulant and analyzed within 2 h. A volume of 50 ml of peripheral blood was then incubated with 10 ml of fluorescein isothiocyanate-or peridinin chlorophyll protein complex (PerCP)-conjugated anti-human CD45 mAb (Becton-Dickinson, BD, San Jose, CA, USA) and with 10 ml phycoerythrin-conjugated antihuman CD34 mAb (BD) or 5 ml of phycoerythrin-conjugated anti-human CD133 mAb (Miltenyi Biotec, Auburn, CA, USA), using the multiparameter flow-cytometric lyse no-wash method PROCOUNT (BD), in a TRUCOUNT tube (BD) with a known number of fluorescent beads.…”

Section: Flow-cytometric Evaluationmentioning

confidence: 99%

Circulating progenitor cells are increased in newly diagnosed untreated hypertensive patients with arterial stiffening but normal carotid intima-media thickness

et al. 2011

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Circulating progenitor cells (CPCs), including endothelial progenitor cells (EPCs), have a key role in endothelium repair. Cellular NADPH oxidase (Nox) enzymes, including Nox-containing gp91phox, represent a source of reactive oxygen species (ROS); ROS trigger protective signals but may also have detrimental effects. Cellular defenses against ROS include the enzymes manganese superoxide dismutase (MnSOD), catalase (CAT) and glutathione peroxidase type-1 (GPx-1). We investigated the relationships of CPCs with cellular gp91phox, MnSOD, CAT, GPx-1 and ROS levels and with carotid intima-media thickness (cIMT) and stiffness in hypertensives without additional risk factors for cardiovascular disease. CPCs from 53 newly diagnosed, untreated hypertensives and from 29 controls were isolated and identified by flow cytometry. gp91phox, MnSOD, CAT, and GPx-1 mRNA and protein expression and ROS generation were evaluated in enriched samples of CD34 + cells; cIMT and stiffness were assessed. Hypertensives showed higher arterial stiffness (Po0.001) but no difference in cIMT with respect to controls. ROS generation was slightly increased (P¼0.04), whereas gp91phox, MnSOD, CAT and GPx-1 were significantly higher (Po0.001) with respect to controls, as was CPC number (Po0.001), but EPCs were no different. CPC and EPC numbers correlated with gp91phox, ROS and fibrinogen (Po0.001); moreover, gp91phox, MnSOD, CAT and GPx-1 were correlated with CPC number. In early phases of arterial hypertension, before the development of wall thickening and even in the presence of arterial mechanical impairment, CPC number may be increased to maintain an adequate number of EPCs in peripheral blood.

show abstract

Concentration of circulating endothelial progenitor cells (EPC) in normal pregnancy and in pregnant women with diabetes and hypertension

Cited by 52 publications

References 35 publications

Maternal Insulin Therapy Increases Fetal Endothelial Progenitor Cells During Diabetic Pregnancy

Maternal Insulin Therapy Increases Fetal Endothelial Progenitor Cells During Diabetic Pregnancy

Genomic and proteomic investigation of preeclampsia

Circulating progenitor cells are increased in newly diagnosed untreated hypertensive patients with arterial stiffening but normal carotid intima-media thickness

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