Concentration of Certain Myxoviruses with Polyethylene Glycol

Polyethylene glycol purification of influenza virus with respect to aggregation and antigenicity

“…This interlocking, induced by the P.E.G., may be responsible for the observed failure of such aggregates to disperse. It is of interest to note that Kanarek & Tribe (1967) found complete quantitative recovery of infectivity and an apparent increase in haemagglutinin activity in some cases after treatment of dilute myxovirus suspensions with P.E.G. This phenomenon they attributed to disaggregation of agglutinated virus particles.…”

“…Influenza virus may be precipitated with polyethylene glycol at polymer concentrations appreciably lower than those used by Kanarek & Tribe (1967). The precipitation curves after storage at 40 and 370 C. for 20 hr.…”

“…at appreciably lower polymer concentration than found by Kanarek & Tribe (1967). As high concentrations of the polymer in influenza vaccines would not meet the approval of Governmental Health agencies it stands to reason that if the content of the polymer is at a low initial value in the precipitate, removal of traces from vaccines would be more complete and more easily achieved.…”

Rubella Virus: Structural and Non-structural Proteins

“…The method helps overcome major problems associated with characterization of RV proteins, namely the fragility of the virus particle, contamination by cell proteins, and relatively low yields in cell culture. PEG 6000 has been used previously to concentrate RV infectious particles (Kanarek & Tribe, 1967) or RV HA (Trudel & Payment, 1980) but the structural proteins were not analysed in these reports. Trudel & Payment (1980) discarded the procedure in favour of hollow fibre ultrafiltration because of the large residue of total protein; this probably represented mainly serum proteins from the culture fluid which were precipitated by the high concentration (10%) + D. S. BOWDEN AND E. G. WESTAWAY of P E G 6000.…”

The rapid concentration and purification of influenza virus from allantoic fluid

“…Several enveloped animal viruses have been sueeesscully concentrated from tissue culture fluid by precipitation with PEG-6000 in the presence of 0.5 M NaC1 (3,6,12,13,16,18). When we used this method to recover influenza virus from infectious egg allantoic fluid, however, a large precipitate formed and the subsequent recovery of virus from the precipitate was minimal.…”