The rapid concentration and purification of influenza virus from allantoic fluid

Heyward, John T.; Klimas, Richard A.; Stapp, Marianne D.; Obijeski, John F.

doi:10.1007/bf01314484

Cited by 35 publications

(31 citation statements)

References 20 publications

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“…A/Brisbane/59/07 was obtained from the Centers for Disease Control and Prevention and propagated for 2 days at 34°C in 10-day embryonated hen's eggs [18]. Castrated and de-scented Fitch ferrets (6-8 months of age, 800-1800 grams; Triple F Farms, Sayre, PA) were assigned to one of 6 treatment groups (Table 1) by a weight-matched computer-generated randomization procedure.…”

Section: Resultsmentioning

confidence: 99%

Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret

McBrayer

Camp

Tapp

et al. 2010

Virol J

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Every year, influenza viruses infect approximately 5-20% of the population in the United States leading to over 200,000 hospitalizations and 36,000 deaths from flu-related complications. In this study, we characterized the immune and pathological progression of a seasonal strain of H1N1 influenza virus, A/Brisbane/59/2007 in a ferret model. The immune response of the animals showed a dose-dependent increase with increased virus challenge, as indicated by the presence of virus specific IgG, IgM, and neutralizing antibodies. Animals infected with higher doses of virus also experienced increasing severity of clinical symptoms and fever at 2 days post-infection (DPI). Interestingly, weight loss was more pronounced in animals infected with lower doses of virus compared to those infected with a higher dose; these results were consistent with viral titers of swabs collected from the nares, but not the throat. Analyzed specimens included nasal and throat swabs from 1, 3, 5, and 7 DPI as well as tissue samples from caudal lung and nasal turbinates. Viral titers of the swab samples in all groups were higher on 1 and 3 DPI and returned to baseline levels by 7 DPI. Analysis of nasal turbinates indicated presence of virus at 3 DPI in all infected groups, whereas virus was only detected in the lungs of animals in the two highest dose groups. Histological analysis of the lungs showed a range of pathology, such as chronic inflammation and bronchial epithelial hypertrophy. The results provided here offer important endpoints for preclinical testing of the efficacy of new antiviral compounds and experimental vaccines.

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Section: Resultsmentioning

confidence: 99%

Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret

McBrayer

Camp

Tapp

et al. 2010

Virol J

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“…Virus from clarified cell culture supernatants was precipitated for at least 2 h at 4°C with molecular weight 6,000 polyethylene glycol (Millipore, Australia), at a final concentration of 8% (14,15). Following precipitation, virus was recovered by centrifugation at 9,000 ϫ g for 15 min.…”

Section: Growth Of Clinical Virus Specimensmentioning

confidence: 99%

Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains

Fernandes

Downard

2014

J Clin Microbiol

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The reemergence of deadly pandemic influenza virus strains has necessitated the development of improved methods for rapid detection and subtyping of influenza viruses that will enable more strains to be characterized at the molecular level. Representative circulating strains of human influenza viruses from primary clinical specimens were grown in cell culture, purified through polyethylene glycol precipitation, proteolytically digested with an endoproteinase, and analyzed and identified by high-resolution mass spectrometry using unique signature peptides that are characteristic of type A H1N1 and H3N2 and type B influenza viruses. This proteotyping approach enabled circulating strains of type A influenza virus to be typed and subtyped, cocirculating seasonal and pandemic H1N1 viruses to be differentiated, and the lineage of type B viruses to be determined through single-ion detection by high-resolution mass spectrometry. Results were obtained using virus titers comparable to those used in reverse transcription (RT)-PCR assays with clinical specimens grown in cell cultures. The methodology represents a more rapid and direct approach than RT-PCR and can be integrated into existing procedures currently used for the surveillance of emerging pandemic and seasonal influenza viruses.

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“…For instance, influenza virus has been separated on a sucrose gradient in the laboratory and on a large scale (11,17). Downstream processing in vaccine production has a great role and is a crucial step.…”

Section: Discussionmentioning

confidence: 99%

“…In 1977, centrifugation on a sucrose gradient was carried out for avian influenza purification (11). Chromatography has been suggested as a novel method in virus purification (9).…”

mentioning

confidence: 99%

Purification of avian influenza virus (H9N2) from allantoic fluidby size-exclusion chromatography

Shirvan¹,

SAMIANIFARD²,

GHODSIAN³

2016

Turk J Vet Anim Sci

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IntroductionAvian influenza (AI) refers to flu caused by type A strains of the influenza virus that is an infectious disease of birds. Influenza viruses are classified into three types: A, B, and C. Only influenza A viruses infects birds. The avian influenza virus (AIV) is a member of the family Orthomyxoviridae, containing negative-sense and singlestranded RNA (1). Based on their pathogenicity, two types of AIV have been described: the highly pathogenic type (HPAIV), which causes severe disease with high mortality, and the low pathogenic type (LPAIV), inducing asymptomatic or mild infection (2).According to the main surface proteins, hemagglutinin (HA) and neuraminidase (NA), influenza A viruses are categorized into different subtypes: 17 for HA and 10 for NA. The HA of the influenza virus is the major surface protein inducing protective immune responses (3). According to the World Health Organization (WHO), all H9 viruses found in wild birds and poultry are LPAIVs.During 1994-1999, infections in poultry that were caused by influenza viruses of the H9 subtype were noticeably prevalent. Outbreaks of the H9N2 subtype occurred in domestic ducks, chickens, and turkeys in Europe and the United States (4). This type of bird flu was reported in 1998 on a broiler chicken farm in Tehran, Iran, and from neighboring provinces (5,6). Vaccines have an

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The rapid concentration and purification of influenza virus from allantoic fluid

Cited by 35 publications

References 20 publications

Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret

Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret

Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains

Purification of avian influenza virus (H9N2) from allantoic fluidby size-exclusion chromatography

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