Concentration‐dependent cellular behavior and osteogenic differentiation effect induced in bone marrow mesenchymal stem cells treated with magnetic graphene oxide

He, Yi; Li, Yiming; Chen, Guanhui; Wei, Changbo; Zhang, Xiliu; Zeng, Binghui; Chen, Yi; Wang, Chao; Yu, Dongsheng

doi:10.1002/jbm.a.36791

Cited by 30 publications

(26 citation statements)

References 47 publications

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“…Magnetic graphene oxide (MGO) decorated by NPs of Fe 3 O 4 was prepared to study cell behavior and the course of osteogenic differentiation, along with the mechanism of the MGO-induced effect, using mesenchymal stem cells derived from rat bone marrow (BMSCs). In conclusion, it was found that MGO, at low concentrations, is biologically compatible and simultaneously notably accelerates osteogenic differentiation in BMSCs [84].…”

Section: Nanotheranostics and Tissue Engineeringmentioning

confidence: 74%

Graphenic Materials for Biomedical Applications

2019

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Graphene-based nanomaterials have been intensively studied for their properties, modifications, and application potential. Biomedical applications are one of the main directions of research in this field. This review summarizes the research results which were obtained in the last two years (2017-2019), especially those related to drug/gene/protein delivery systems and materials with antimicrobial properties. Due to the large number of studies in the area of carbon nanomaterials, attention here is focused only on 2D structures, i.e. graphene, graphene oxide, and reduced graphene oxide.

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Section: Nanotheranostics and Tissue Engineeringmentioning

confidence: 74%

Graphenic Materials for Biomedical Applications

2019

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“…Figure 6a illustrates the cell morphology onto the BS, positive control (0.1% gelatin‐coating), and all scaffolds at different time intervals (24, 48, and 72 h). The attached cells showed ideal cell morphology for the MC3T3‐E1 cell line in which cells were cylindrical with proper cell bodies (He et al., 2020; Khan et al., 2020a). The cells adhered to the bottom of the well have relatively less retained morphologies than the positive control.…”

Section: Resultsmentioning

confidence: 99%

Arabinoxylan/graphene‐oxide/nHAp‐NPs/PVA bionano composite scaffolds for fractured bone healing

Khan

Haider

Razak

et al. 2021

J Tissue Eng Regen Med

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The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration. This work is focused on this issue while not compromising on the properties of a scaffold for bone regeneration. Biocomposite scaffolds (BS) were fabricated via the freeze–drying technique. The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform‐infrared [FT‐IR]), microscopic (scanning electron microscope [SEM]), X‐ray (powder X‐ray diffraction and energy‐dispersive X‐ray), and other analytical (Brunauer–Emmett–Teller, universal testing machine Instron) techniques. Antibacterial, cellular, and hemocompatibility assays were performed using standard protocols. FT‐IR confirmed the interactions of all the components. SEM illustrated porous and interconnected porous morphology. The percentage porosity was in the range of 49.75%–67.28%, and the pore size was 215.65–470.87 µm. The pore size was perfect for cellular penetration. Thus, cells showed significant proliferation onto these scaffolds. X‐ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO). The cell viability was 85%–98% (BS1–BS3), which shows no significant toxicity of the biocomposite. Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis. The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content. These exciting results revealed that this material has the potential for possible application in bone tissue engineering.

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“…57 The concentration of GDs greater than 10 μg/mL might inhibit the proliferation of bone marrow mesenchymal stem cells. 58 Functionalization could decrease toxicity potential of GDs to some extent. 59 In addition, the GDs in sheet structures 60 or compounded with other materials 61 could bring lower toxicity risk because of decreased possibility in penetration into the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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Concentration‐dependent cellular behavior and osteogenic differentiation effect induced in bone marrow mesenchymal stem cells treated with magnetic graphene oxide

Cited by 30 publications

References 47 publications

Graphenic Materials for Biomedical Applications

Graphenic Materials for Biomedical Applications

Arabinoxylan/graphene‐oxide/nHAp‐NPs/PVA bionano composite scaffolds for fractured bone healing

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

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