Concentration-associated pathology of alkali burn in a mouse model using anterior segment optical coherence tomography with angiography

Luisi, Jonathan; Lin, Jonathan; Karediya, Nishad; Kraft, E; Sharifi, Ardalan; Schmitz-Brown, Mary; Zhang, Wenbo; Ameredes, Bill T.; Merkley, Kevin H.; Gupta, Praveena

doi:10.1016/j.exer.2022.109210

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…It could be deduced that disruption of the corneal epithelium or endothelium will cause corneal edema, but endothelial disruption may lead to more intense edema than epithelial disruption. This finding was also reported by Luisi et al They found that the degree of corneal edema was significantly higher in severe burns involving corneal endothelial structures than in mild burns involving only the superficial layers of the cornea after alkali burns in mice 25 …”

Section: Discussionsupporting

confidence: 85%

“…This finding was also reported by Luisi et al They found that the degree of corneal edema was significantly higher in severe burns involving corneal endothelial structures than in mild burns involving only the superficial layers of the cornea after alkali burns in mice. 25 The OCT results showed that the regenerated epithelium appeared at approximately 6 h postexposure and completely covered the damaged area in the following 30 h for both laser groups. Subsequently, the regenerated epithelium gradually increased to normal thickness.…”

Section: Discussionmentioning

confidence: 90%

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Corneal wound healing following infrared laser irradiation at the wavelengths of 1.319 and 10.6 μm

Zhou,

Yin,

Wang

et al. 2023

Lasers Surg Med

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ObjectivesTo investigate the wound healing of rabbit cornea following infrared laser irradiations at the wavelengths of 1.319 and 10.6 μm.Materials and MethodsTwelve New Zealand rabbits were selected to establish a corneal injury model. The right and left eyes were irradiated with a neodymium‐doped yttrium aluminum garnet laser at the wavelength of 1.319 μm (140 J/cm2) for 0.7 s and a CO2 laser at the wavelength of 10.6 μm (5.94 J/cm2) for 0.14 s, respectively. The incident spot diameter was 3 mm. Optical coherence tomography (OCT) was used to monitor injuries at 0 h, 0.5 h, 1 h, 3 h, 6 h, 12 h, 18 h, 24 h, 30 h, 36 h, 42 h, 48 h, 54 h, 60 h, 66 h, 3 d, 7 d, 14 d, 28 d, 3 m, and 6 m postexposure. Meanwhile, slit‐lamp microscopy and histopathology were performed at 6 h, 24 h, 3 d, 7 d, 14 d, 28 d, 3 m, and 6 m postexposure.ResultsAfter the two types of infrared laser injuries, distinct white circular lesions on the corneal surface were directly observed. Deeper corneal injury, more severe edema, and faster migration of new epithelium were found for the wavelength of 1.319 μm, compared to the wavelength of 10.6 μm.ConclusionsOCT combined with histopathology and slit‐lamp microscopy can clearly observe the dynamic process of corneal wound healing after infrared laser irradiation. The damage characteristics for the two different wavelengths were visibly different, but the whole wound healing process was similar. The obtained results may provide references for the diagnosis, treatment, and evaluation of laser‐induced damages.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 90%

Corneal wound healing following infrared laser irradiation at the wavelengths of 1.319 and 10.6 μm

Zhou,

Yin,

Wang

et al. 2023

Lasers Surg Med

View full text Add to dashboard Cite

show abstract

“…Alkali injury, which is frequently used in animal studies, results in deep tissue destruction and inflammation. 7 Basic alkaline compounds are more lipophilic, which allows them to penetrate and damage the eye extensively and induce inflammation in the deep structures of the anterior segment, such as the trabecular meshwork, ciliary body, lens, or even retina. 8 – 11 …”

Section: Introductionmentioning

confidence: 99%

Regenerated Corneal Epithelium Expresses More βIII-Tubulin After Chemical Injuries Compared to Mechanical Injuries

Shadmani,

Ercal,

Uzun

et al. 2023

Trans. Vis. Sci. Tech.

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Purpose Defining the regenerative response following various types of corneal chemical and mechanical injuries is important for understanding the pathophysiology of the injury and evaluating the effectiveness of the therapies. This study characterizes corneal epithelial healing in a murine chemical and mechanical injury model. Methods Four groups of 10 mice each received complete corneolimbal injuries by AlgerBrush, AlgerBrush/thermal, NaOH (0.5 N), or ethanol. Slit-lamp and optical coherence tomography examinations were performed daily for 14 days. Corneal opacity (CO) and neovascularization (NV) were evaluated. The origin of the regenerated epithelium was illustrated by anti-cytokeratin 12 (K12) and anti-K13. The height of regenerated corneal epithelium and intraepithelial free nerve endings (FNEs) stained with anti–βIII-tubulin were measured. The amount of fibrosis was measured by anti–α-smooth muscle actin (α-SMA) monoclonal antibody in the different groups. Statistical analysis was performed by ANOVA and t -test. Results Corneal opacity and neovascularization were markedly higher in the NaOH and AlgerBrush/thermal groups. Molecular studies revealed the following: Regenerated corneal epithelium thickness was less than normal in all groups, the AlgerBrush group had the shortest height of the regenerated epithelium, βIII-tubulin was expressed in the entire height of corneal epithelium in all groups except in the AlgerBrush group, and K12 was replaced by K13 in all groups. Conclusions Corneal wound healing is more effective following chemical injuries in terms of epithelial thickness. Inflammation may play an important role in the outcome. Translational Relevance Inflammation following different injuries may be redirected to be more effective in corneal regeneration and clarity.

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Corneal fibrosis: From in vitro models to current and upcoming drug and gene medicines

Trujillo Cubillo,

Gurdal,

Zeugolis

2024

Advanced Drug Delivery Reviews

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Concentration-associated pathology of alkali burn in a mouse model using anterior segment optical coherence tomography with angiography

Cited by 5 publications

References 41 publications

Corneal wound healing following infrared laser irradiation at the wavelengths of 1.319 and 10.6 μm

Corneal wound healing following infrared laser irradiation at the wavelengths of 1.319 and 10.6 μm

Regenerated Corneal Epithelium Expresses More βIII-Tubulin After Chemical Injuries Compared to Mechanical Injuries

Corneal fibrosis: From in vitro models to current and upcoming drug and gene medicines

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