Concentrated MTA Repair HP reduced biofilm and can cause reparative action at a distance

Abstract

Aim To evaluate in vitro whether MTA Repair HP can induce repair processes at a distance, including its effects on biofilm, cell viability, migration, production of TGF‐β, phosphate and ALP, evaluated through MTA diluted extracts. Methodology Initially, antibacterial tests were performed with the bacterium Streptococcus mutans (ATCC 25175) in the presence of MTA extracts (dilutions of 1:1, 1:2 and 1:4). Growth inhibition assay by microdilution in broth, antibiofilm plate assay of young biofilm and antibiofilm assay in confocal microscopy of mature biofilm were carried out. Then, pulp cells were stimulated in the presence of several MTA dilutions, and cell viability (MTT assay), proliferation and migration capacity (scratch assay) were evaluated. To evaluate the capacity of 1:1, 1:2 and 1:4 dilutions of MTA Repair HP to promote the production of important agents of odontogenic differentiation and mineralization, ALP activity, TGF‐β secretion and phosphate quantification were measured. Statistical differences were verified using one‐way and two‐way anova and Tukey's post‐tests. Results The test dilutions of MTA Repair HP did not inhibit planktonic S. mutans growth but were able to reduce young and mature S. mutans biofilm (p < 0.001). In addition, none of the MTA Repair HP dilutions was cytotoxic for pulp cells. The 1:2 and 1:4 dilutions of MTA Repair HP induced migration and proliferation of pulp cells (p < 0.05). ALP activity and TGF‐β secretion were independent of the tested dilution (p < 0.001). Diluted 1:4 MTA Repair HP produced less phosphate than the more concentrated 1:1 and 1:2 MTA dilutions (p < 0.001). Conclusions Undiluted MTA Repair HP reduced S. mutans biofilm, when compared to 1:2 and 1:4 MTA dilutions. Furthermore, none of the tested dilutions was cytotoxic to pulp cells. MTA Repair HP promoted cell migration and proliferation at a distance, assessed through the dilution of the MTA. Even from a distance, MTA Repair HP has the ability to participate in some events related to repair, such as migration, proliferation and TGF production.

Host defense peptides combined with MTA extract increase the repair in dental pulp cells: in vitro and ex vivo study

“…Our findings demonstrated that although HDPs, MTA or a combination of both did not cause inhibition of S. mutans growth under planktonic conditions, these materials have the potential of reducing biofilm performed in 24 h and biofilm performed over 7 days biofilms of S. mutans . Our group also recently demonstrated the ability of MTA extracts to reduce biofilm performed in 24 h and S. mutans biofilm performed over 7 days 32 . Besides, previous studies have demonstrated that the antibiofilm capability of IDR1018 is directly related to its concentration 33 , 34 .…”

“…Previous studies have not shown any antibacterial effect of MTA on S. mutans under planktonic conditions 30 – 32 . Our findings demonstrated that although HDPs, MTA or a combination of both did not cause inhibition of S. mutans growth under planktonic conditions, these materials have the potential of reducing biofilm performed in 24 h and biofilm performed over 7 days biofilms of S. mutans .…”

Cytotoxicity and Antibacterial Activity of Mineral Trioxide Aggregate Cement with Radiopacity Introduced by ZrO2

“…The results of our preliminary studies on the biomaterials indicate a suppressive effect of ZrO 2 enriched MTA cements on the growth of Streptococcus mutans and Streptococcus sanguinis in suspension. Silva et al [ 52 ] showed that MTA repair HA cement significantly inhibited Streptococcus mutans biofilm formation, however, extracts from this biomaterial did not lower bacterial numbers in suspension. The physicochemical properties of biomaterials affect their antibacterial activity.…”

Biological and chemical properties of five mineral oxides and of mineral trioxide aggregate repair high plasticity: an in vitro study

“…MTA Repair HP has antimicrobial action against E. faecalis 20 ; however, a more recent study showed that although MTA Repair HP did not inhibit the planktonic growth of S. mutans , it did reduce S. mutans biofilm formation 21 . In the present study, MTA Repair HP was effective against all microorganisms evaluated except P. endodontalis .…”