Biological and chemical properties of five mineral oxides and of mineral trioxide aggregate repair high plasticity: an in vitro study

Abstract

Calcium silicate-based cements have diverse applications in endodontics. This study aimed to evaluate the antibiofilm action, biocompatibility, morphological structure, chemical composition and radiopacity of Five Mineral Oxides (5MO), Mineral Trioxide Aggregate Repair High Plasticity (MTA Repair HP), and Mineral Trioxide Aggregate (MTA) cements. MTT analysis was used to test the antibiofilm action of these cements against five anaerobic microorganisms, and test their biocompatibility with mouse macrophage (RAW 264.7) and osteoblasts (MG-63) cultures. Their morphological structure and chemical composition were evaluated by scanning electron microscopy (SEM) coupled to energy dispersion X-ray spectroscopy (EDX), and the phase analysis was performed by X-ray diffraction (XRD). Conventional radiography was used to assess the radiopacity of the cements. 5MO, MTA Repair HP and MTA were effective against Porphyromonas gingivalis, Parvimonas micra, Fusobacterium nucleatum and Prevotella intermedia, they were biocompatible with macrophages and osteoblasts after 5 min of contact, and they had adequate radiopacity to be used clinically. Bismuth oxide (Bi2O3) is used as a radiopacifier in MTA and 5MO, and calcium tungstate, in MTA Repair HP. Titanium dioxide (TiO2) (ANATASE) is responsible for the antimicrobial action and biocompatibility of 5MO.

Antimicrobial and Antibiofilm Effect of Brazilian Green Propolis Aqueous Extract against Dental Anaerobic Bacteria

“…MMC values were determined, and 10 µL of MIC and a concentration above and below MIC were seeded in Brucella agar. After incubation (48 h/37 °C), MMC corresponded to the well with no grown bacteria and with the lowest concentration of BGP-AqExt [ 4 , 41 ].…”

“…Immediately after, the microplates were incubated for 10 min at 37 °C and then were agitated in vortex for additional 10 min at room temperature. Finally, a spectrophotometer (Bio-Tek Instruments, Winooski, VT, USA) was used to measure the absorbance of the wells at 570 nm, and data generated were converted to cell viability percentage [ 41 ].…”

Biomimetic approaches and materials in restorative and regenerative dentistry: review article

“…Mineral Trioxide Aggregate material with very high plasticity (MTA Repair HP) has been recently launched, representing a calcium silicate-based cement with enhanced physical and mechanical properties. It is highly biocompatible with human pulp stem cells and with a proven bio-mineralization and antimicrobial activity [80,81].…”

Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA

“…Due to its excellent chemical, physical and biological properties, in particular its biocompatibility and osteoconductive potential, it has been used in dentistry for over 20 years [ 10 , 11 , 14 , 15 , 16 , 17 , 18 ]. MTA is used in endodontic treatments [ 13 , 19 , 20 ] and is also recommended in vital pulp therapy to preserve the vitality of dental pulp [ 13 , 19 , 20 ]. Several studies have demonstrated the antibacterial and antifungal properties of this material [ 19 , 20 , 21 , 22 ].…”

“…MTA is used in endodontic treatments [ 13 , 19 , 20 ] and is also recommended in vital pulp therapy to preserve the vitality of dental pulp [ 13 , 19 , 20 ]. Several studies have demonstrated the antibacterial and antifungal properties of this material [ 19 , 20 , 21 , 22 ]. Despite the potential of MTA as an antibacterial agent, there are no studies on its use in implantology [ 23 ].…”