Concanavalin A immobilized magnetic poly(glycidyl methacrylate) beads for prostate specific antigen binding

İdil, Neslihan; Perçin, Işık; Karakoç, Veyis; Yavuz, Handan; Aksöz, Nilüfer; Deni̇zli̇, Adil

doi:10.1016/j.colsurfb.2015.06.050

Cited by 19 publications

(11 citation statements)

References 36 publications

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“…Albumin and immunoglobulin G free diluted serum samples of patients with prostate cancer were incubated with 10 mg of each type of MBs for 2 h at 25 °C at a stirring rate of 100 rpm. The binding capacities of each type of MBs were calculated from the difference between initial and final tPSA and fPSA concentrations by using the Equation 4 [105].

Q = \frac{false[false(italicCi - italicCf false) false] italicx V false]}{m}

where Q is the amount of PSA bound onto unit mass of the mPGMA-ConA and the mPGMA-HMDA-Con A beads (ng/g), Ci and Cf are the initial and final PSA concentrations (ng/mL), respectively, V is the volume of aqueous phase (mL) and m is the amount of adsorbent used (g) [105].…”

Section: Chromatography-based Assaysmentioning

confidence: 99%

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Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Moraes¹,

Cardoso²,

Seidl³

et al. 2016

CPD

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Affinity-based chromatography assays encompass the use of solid supports containing immobilized biological targets to monitor binding events in the isolation , identification and/or characterization of bioactive compounds. This powerful bioanalytical technique allows the screening of potential binders through fast analyses that can be directly performed using isolated substances or complex matrices. An overview of the recent researches in frontal and zonal affinity-based chromatography screening assays, which has been used as a tool in the identification and characterization of new anti-cancer agents, is discussed. In addition, a critical evaluation of the recently emerged ligands fishing assays in complex mixtures is also discussed.

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Q = \frac{false[false(italicCi - italicCf false) false] italicx V false]}{m}

Section: Chromatography-based Assaysmentioning

confidence: 99%

“…The mPGMA-HDMA-Con A beads could be reused without a remarkable decrease in the binding capacities after 5 binding-desorption cycles. The mPGMA-HDMA-Con A beads could be useful for the detection of PSA and suggested as a model system for other glycoprotein biomarkers [105]. …”

Section: Chromatography-based Assaysmentioning

confidence: 99%

Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Moraes¹,

Cardoso²,

Seidl³

et al. 2016

CPD

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“…36 Tang et al demonstrated Con A-immobilized magnetic NPs for the selective separation of glycoproteins. 37 Con A-immobilized AuNPs, 38 silica-based lectin microcoulumns, 39 Con A-immobilized magnetic poly(glycidylmethacrylate) (mPGMA) beads, 40 Con A affinity chromatography, and Con A-Sepharose have also been employed for the selective enrichment of glycoproteins. [41][42][43] We have previously reported MALDI-MS and dimethyl labeling techniques to quantify the CP4 EPSPS protein in GM soya.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry combined with affinity probes for the identification of CP4 EPSPS in genetically modified soybeans

Devi

Chu

et al. 2019

J Mass Spectrom

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Sample preparation methods used for genetically modified organisms (GMOs) analysis are often time consuming, require extensive manual manipulation, and result in limited amounts of purified protein, which may complicate the detection of low‐abundance GM protein. A robust sample pretreatment method prior to mass spectrometry (MS) detection of the transgenic protein (5‐enolpyruvylshikimate‐3‐phosphate synthase [CP4 EPSPS]) present in Roundup Ready soya is investigated. Liquid chromatography‐multiple reaction monitoring tandem MS (nano LC‐MS/MS‐MRM) was used for the detection and quantification of CP4 EPSPS. Gold nanoparticles (AuNPs) and concanavalin A (Con A)‐immobilized Sepharose 4B were used as selective probes for the separation of the major storage proteins in soybeans. AuNPs that enable the capture of cysteine‐containing proteins were used to reduce the complexity of the crude extract of GM soya. Con A‐sepharose was used for the affinity capture of β‐conglycinin and other glycoproteins of soya prior to enzymatic digestion. The methods enabled the detection of unique peptides of CP4 EPSPS at a level as low as 0.5% of GM soya in MRM mode. Stable‐isotope dimethyl labeling was further applied to the quantification of GM soya. Both probes exhibited high selectivity and efficiency for the affinity capture of storage proteins, leading to the quantitative detection at 0.5% GM soya, which is a level below the current European Union's threshold for food labeling. The square correlation coefficients were greater than 0.99. The approach for sample preparation is very simple without the need for time‐consuming protein prefractionation or separation procedures and thus presents a significant improvement over existing methods for the analysis of the GM soya protein.

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“…calcium, Ca 2+ ) and transition metal ions (manganese (Mn 2+ ) to activate the binding site for the interaction with the mannosyl and glucosyl. Con A have been used several years for the detection and purification of glycoproteins and sugars in the field of diagnosis and separation [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract

Çimen

Bereli

Andaç

et al. 2018

Separation Science Plus

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Concanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N‐Methacryloyl‐l‐histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non‐imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis.

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Concanavalin A immobilized magnetic poly(glycidyl methacrylate) beads for prostate specific antigen binding

Cited by 19 publications

References 36 publications

Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Mass spectrometry combined with affinity probes for the identification of CP4 EPSPS in genetically modified soybeans

Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract

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