Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract

Abstract

Concanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N‐Methacryloyl‐l‐histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non‐imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis.

Detection of amoxicillin residues in egg extract with a molecularly imprinted polymer on gold microchip using surface plasmon resonance and quartz crystal microbalance methods

“…3.2.3 Selectivity studies of AMOX imprinted SPR and QCM sensors. Selectivity study, which is known as the ability to detect the target in the sensing system, is one of the most important parameters of molecular imprinting (Çimen et al, 2018;Hüseynli et al, 2019). Selectivity studies were made by β-lactam antibiotics CEP and AMP against AMOX, and the results are shown in Figure 4.…”

“…The molecular orientation and mechanical properties of molecularly imprinted polymers present both low cost and efficient approach for synthetic recognition (Çimen, Bereli, Andaç, & Denizli, 2018;Guan, Liu, Wang, & Zhang, 2008;Rafaela et al, 2011). The main purpose of the molecular imprinting technique is obtaining the target molecule with high selectivity by creating specific regions for that molecule.In pharmaceuticals and many other natural sciences, the use of molecularly imprinted polymers (MIPs) is one step ahead as a detection agent because of this unique technique (Ashley et al, 2017).…”

Advances in the development of molecularly imprinted polymers for the separation and analysis of proteins with liquid chromatography

“…A brief synopsis of the different types of protein and biomacromolecular surface‐imprinted monoliths and particles/beads is shown in Tables and , respectively, including the various surface imprinting strategies and characterization techniques that have been utilized.…”

“…They create template cavities directly at the liquid-solid film interface of a polymer, thereby permitting more efficient template removal, faster binding kinetics, and improved binding site accessibility. A brief synopsis of the different types of protein and biomacromolecular surface-imprinted monoliths [75][76][77][78][79][80][81][82][83][84] and particles/beads is shown in Tables 3 and 4, respectively, including the various surface imprinting strategies and characterization techniques that have been utilized.…”

Surface Plasmon Resonance Nanosensors for Detecting Amoxicillin in Milk Samples with Amoxicillin Imprinted Poly(hydroxyethyl methacrylate‐N‐methacryloyl‐(L)‐ glutamic acid)

“…Molecularly imprinted polymer (MIP) method employs a functional monomer and a cross‐linker, making the polymerization surrounding the mold molecule in the solvent . The objective of the MIP technique is to specific regions for target molecule in the polymer and therefore increase the selectivity of the molecule . Due to these superior properties, MIP method is widely used in many different fields such as separation, drug and chemical determination, catalysis based on sensor .…”