Computer Simulations ofViscosity Dependent MolecularRelaxation Processes

Kaschke, M.; Kleinschmidt, J.; Wilhelmi, B.

doi:10.1155/lc.5.119

Cited by 17 publications

(16 citation statements)

References 7 publications

(7 reference statements)

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“…This value compared well with the value of 2/3 predicted by Forster and Hoffmann 26 . Typical slope values range from 0.2 to 1.4 for different rotors 27 . We found that below 20 cP, the fluorescence lifetime and quantum yield were minimally variant.…”

Section: Resultsmentioning

confidence: 99%

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Raut

Kimball

Fudala

et al. 2014

Phys. Chem. Chem. Phys.

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Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependant behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside cytoplasm thereby allowing viscosity mapping of internal cell components.

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Section: Resultsmentioning

confidence: 99%

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Raut

Kimball

Fudala

et al. 2014

Phys. Chem. Chem. Phys.

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“…12). Typical γ -values range between 0.2 and 1.4 97. Fluorescent compounds with high lifetime sensitivity to viscosity are called molecular rotors 98-101.…”

Section: Theory Of Fluorescence Lifetime and Processes Affecting Flmentioning

confidence: 99%

Fluorescence Lifetime Measurements and Biological Imaging

2010

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“…A plot of log F f , or log t f as a function of log h should yield a straight line with a slope of a [62,63] which serves as a calibration graph to convert F f or t f into h.…”

Section: Development Of the Theoretical Background: Fluorescence Anismentioning

confidence: 99%

Fluorescence Anisotropy of Molecular Rotors

et al. 2011

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We present polarization-resolved fluorescence measurements of fluorescent molecular rotors 9-(2-carboxy-2-cyanovinyl)julolidine (CCVJ), 9-(2,2-dicyanovinyl)julolidine (DCVJ), and a meso-substituted boron dipyrromethene (BODIPY-C(12)). The photophysical properties of these molecules are highly dependent on the viscosity of the surrounding solvent. The relationship between their quantum yields and the viscosity of the surrounding medium is given by an equation first described and presented by Förster and Hoffmann and can be used to determine the microviscosity of the environment around a fluorophore. Herein we evaluate the applicability of molecular rotors as probes of apparent viscosity on a microscopic scale based on their viscosity dependent fluorescence depolarization. We develop a theoretical framework, combining the Förster-Hoffmann equation with the Perrin equation and compare the dynamic ranges and usable working regimes for these dyes in terms of utilising fluorescence anisotropy as a measure of viscosity. We present polarization-resolved fluorescence spectra and steady-state fluorescence anisotropy imaging data for measurements of intracellular viscosity. We find that the dynamic range for fluorescence anisotropy for CCVJ and DCVJ is significantly lower than that of BODIPY-C(12) in the viscosity range 0.6<η<600 cP. Moreover, using steady-state anisotropy measurements to probe microviscosity in the low (<3 cP) viscosity regime, the molecular rotors can offer a better dynamic range in anisotropy compared with a rigid dye as a probe of microviscosity, and a higher total working dynamic range in terms of viscosity.

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Computer Simulations ofViscosity Dependent MolecularRelaxation Processes

Cited by 17 publications

References 7 publications

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Fluorescence Lifetime Measurements and Biological Imaging

Fluorescence Anisotropy of Molecular Rotors

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