Fluorescence Lifetime Measurements and Biological Imaging

Berezin, Mikhail Y.; Achilefu, Samuel

doi:10.1021/cr900343z

Cited by 1,981 publications

(1,804 citation statements)

References 566 publications

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“…Collagen is known to have a narrower, blue-shifted emission spectra and higher τ value when compared with elastin. 8 Consequently, the τ values are higher (p < 0.05) over the grafted region for shorter wavelengths (6.27 AE 0.09 ns, 395 to 410 nm band) when compared with normal tissue (6.01 AE 0.08 ns, 395 to 410 nm band). The trend reverses for higher wavelengths owing to higher fluorescence signal contribution from cellular components and elastin in normal tissue.…”

Section: Resultsmentioning

confidence: 91%

Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

et al. 2014

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Section: Resultsmentioning

confidence: 91%

Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

et al. 2014

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“…The fluorescence lifetime can be a function of viscosity, temperature, pH, ion or oxygen concentrations, glucose, refractive index or polarity, and of interaction with other molecules, e.g. via Förster resonance energy transfer (FRET) [48][49][50][51]. Autofluorescence lifetime measurements can show the metabolic state of cells, and are increasingly used in clinical diagnostics.…”

Section: Fluorescence Microscopy and Fluorescence Lifetime Imaging (Fmentioning

confidence: 99%

Wide-field TCSPC: methods and applications

Hirvonen

Suhling

2016

Meas. Sci. Technol.

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“…The techniques can further be refined by recording not only the fluorescence intensity or the fluorescence spectrum but also the fluorescence decay in the individual pixels of the image. The advantage of lifetime recording is that molecular parameters are directly represented by the parameters of the fluorescence decay functions [1][2][3][4][5] and that the results are independent of the fluorophore concentration which is difficult to control in cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

A wide-field TCSPC FLIM system based on an MCP PMT with a delay-line anode

Becker

Hirvonen

Milnes

et al. 2016

Review of Scientific Instruments

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We report on the implementation of a wide-field time-correlated single photon counting (TCSPC) method for fluorescence lifetime imaging (FLIM). It is based on a 40 mm diameter crossed delay line anode detector, where the readout is performed by three standard TCSPC boards. Excitation is performed by a picosecond diode laser with 50 MHz repetition rate. The photon arrival timing is obtained directly from the microchannel plates, with an instrumental response of ∼190 to 230 ps full width at half maximum depending on the position on the photocathode. The position of the photon event is obtained from the pulse propagation time along the two delay lines, one in x and one in y. One end of a delay line is fed into the “start” input of the corresponding TCSPC board, and the other end is delayed by 40 ns and fed into the “stop” input. The time between start and stop is directly converted into position, with a resolution of 200–250 μm. The data acquisition software builds up the distribution of the photons over their spatial coordinates, x and y, and their times after the excitation pulses, typically into 512 × 512 pixels and 1024 time channels per pixel. We apply the system to fluorescence lifetime imaging of cells labelled with Alexa 488 phalloidin in an epi-fluorescence microscope and discuss the application of our approach to other fluorescence microscopy methods.

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Fluorescence Lifetime Measurements and Biological Imaging

Cited by 1,981 publications

References 566 publications

Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

Wide-field TCSPC: methods and applications

A wide-field TCSPC FLIM system based on an MCP PMT with a delay-line anode

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