Computer simulation of electroinjection analysis and electrophoretically mediated microanalysis

Andreev, Victor P.; Pliss, N. S.

doi:10.1016/s0021-9673(99)00304-0

Cited by 13 publications

(14 citation statements)

References 7 publications

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“…Longer reaction tubes cause expansion of the product dispersion owing to the nonuniform flow profile. In addition to the effective mix of analyte and reagent in EIA and EMMA and the inherent sensitivity derived from the lack of dispersion, the product formed can be concentrated at the boundary of the reagent due to the effect of kinematic focusing theoretically predicted [15,19,21] and then experimentally verified. Kinematic focusing occurs when the reagent concentration is much higher than that of the analyte and when the electrophoretic mobility of the reaction product is close or equal to the electrophoretic mobility of the reagent.…”

Section: Emma and Eiamentioning

confidence: 97%

“…The foundations of these two approaches can be established by resorting to flow-injection analysis (FI), as considered by some research groups as predecessor of both [11,18,19]. The improvement of sample and reagent mixing without an increase in product dispersion in FI is the key for maximizing sensitivity, especially in the case of rapid kinetics analysis.…”

Section: Emma and Eiamentioning

confidence: 99%

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Dual injection capillary electrophoresis: Foundations and applications

Priego-Capote

Castro

2004

Electrophoresis

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The state of the art of capillary electrophoresis (CE) approaches based on dual injection is here reported. Dual injection strategies have been proposed with three main objectives: (i) to provide information about reaction kinetics and/or related parameters, (ii) to perform in-capillary derivatization for improving separation and/or determination, (iii) to develop electrophoretic methods for the simultaneous analysis of anionic and cationic compounds. For the first two purposes, dual injection, which involves sample and reagent, can be realized either from the same end of the capillary (electrophoretically mediated microanalysis, EMMA) or from the two ends of the capillary (electroinjection analysis, EIA). The third objective, with dual injection of sample from the two ends of the capillary, takes advantage of moving cationic and anionic compounds with opposite directions. The foundations of each alternative, conditions necessary for working with them, restrictions, applications as well as perspectives are reviewed in order to establish the advantages, shortcomings, and convenience or no of their use in comparison to conventional CE.

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Section: Emma and Eiamentioning

confidence: 97%

Section: Emma and Eiamentioning

confidence: 99%

Dual injection capillary electrophoresis: Foundations and applications

Priego-Capote

Castro

2004

Electrophoresis

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“…It is worth mentioning that another variant of analyte and reagent mixing called EIA was proposed by Andreev et al [7,34,35], based on the same idea as EMMA. EIA and EMMA are a pair of mutually complementary methods of chemical analysis that can be realized with the help of a CE instrument.…”

Section: Eiamentioning

confidence: 99%

Advances in capillary electrophoretically mediated microanalysis: An update

2006

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This review, as a continuation of an earlier report, gives an overview of recent developments, over the period from 2003 until now, in the use of capillary electrophoretic techniques for the in-line study of enzymatic reactions, derivatization, and chemical reactions. The article is divided into two parts: (i) in-line enzymatic reactions and (ii) in-line derivatization and chemical reactions. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. A literature overview on enzymatic reactions is provided. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization and chemical reactions based on EMMA. Reported derivatization and chemical reaction procedures are discussed and summarized.

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“…In contrast to the dynamic models referred to above, this approach is based upon the modeling of the trajectories of each individual molecule, requires extremely powerful computers in order to compute the motion of a statistically significant number of molecules and is not further considered in this review. Other theoretical work absent from this review includes (i) a stochastic model describing the consequences of wall adsorption in CE [84], (ii) the temperature-dependent interconversion models of dynamic electrophoresis [85][86][87][88], (iii) the simulation model for electroinjection analysis and electrophoretically mediated microanalysis [89], (iv) the affinity electrophoresis models of Andreev et al [90] and Fang and Chen [91][92][93][94], which describe affinity interactions in CE under simplified electromigration conditions, (v) the models of Cann and coworkers describing interacting systems in ZE [95], MBE [96] and IEF [97][98][99], (vi) the models predicting analyte separation in CEC [100][101][102][103][104], (vii) all multi-dimensional models that describe electrokinetically driven mass transport and separations in microfabricated chip devices, such as those of Ermakov et al [105], Bianchi et al [106], Chatterjee [107], Sounart and Baygents [108], Datta and Ghosal [109] and Hirokawa et al [110], (viii) the model of electrokinetic sample injection for capillary CZE with consideration of the electrode configuration [111], (ix) the models that predict sample zone formation, distortion and solute separation in continuous flow electrophoresis [112,113] and recycling electrophoresis [114][115][116], (x) the models describing off-gel electrophores...…”

Section: Introductionmentioning

confidence: 99%

Dynamic computer simulations of electrophoresis: A versatile research and teaching tool

et al. 2010

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Software is available, which simulates all basic electrophoretic systems, including moving boundary electrophoresis, zone electrophoresis, ITP, IEF and EKC, and their combinations under almost exactly the same conditions used in the laboratory. These dynamic models are based upon equations derived from the transport concepts such as electromigration, diffusion, electroosmosis and imposed hydrodynamic buffer flow that are applied to user-specified initial distributions of analytes and electrolytes. They are able to predict the evolution of electrolyte systems together with associated properties such as pH and conductivity profiles and are as such the most versatile tool to explore the fundamentals of electrokinetic separations and analyses. In addition to revealing the detailed mechanisms of fundamental phenomena that occur in electrophoretic separations, dynamic simulations are useful for educational purposes. This review includes a list of current high-resolution simulators, information on how a simulation is performed, simulation examples for zone electrophoresis, ITP, IEF and EKC and a comprehensive discussion of the applications and achievements.

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Computer simulation of electroinjection analysis and electrophoretically mediated microanalysis

Cited by 13 publications

References 7 publications

Dual injection capillary electrophoresis: Foundations and applications

Dual injection capillary electrophoresis: Foundations and applications

Advances in capillary electrophoretically mediated microanalysis: An update

Dynamic computer simulations of electrophoresis: A versatile research and teaching tool

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