Computer-based mass measurement of fragment ion spectra from tandem magnetic sector mass spectrometers with an electrically floated collision cell

Rk, Boyd; Pa, Bott; Br, Beer; Dj, Harvan; Hass, Viviane

doi:10.1021/ac00128a039

Cited by 11 publications

(2 citation statements)

References 23 publications

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“…MIKES analyses with a potential applied to the gas collision cell were initially performed using doublefocusing instruments to study specific CAD processes (see, for example, refs [24][25][26][27][28][29]. Floating the gas collision cell was subsequently shown to improve resolution of product ions [18] and increase the transmission efficiency of low-mass (relative to the precursor) product ions transmitted through the electric sector analyzer [30]. Following the development of four-sector…”

mentioning

confidence: 99%

Determination of high-energy fragmentation of protonated peptides using a beqq hybrid mass spectrometer

Burlet

Orkiszewski

Gaskell

1993

J. Am. Soc. Mass Spectrom.

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A hybrid tandem instrument of BEqQ geometry was used to determine high-energy decomposition of protonated peptides, such as side-chain fragmentation yielding d n and w n ions. The transmission through both E and Q of such product ions, formed in the second field-free region, permits improved mass resolution and confident mass assignment. The experimental technique may involve synchronous scanning of E and Q, or, for the purpose of identification of specific products, limited-range scanning of either E or Q with the other analyzer fixed. These techniques are not equivalent, with respect to product ion transmission, to the double focusing of product ions achieved with four-sector instruments but nevertheless represent a critical improvement over conventional mass-analyzed ion kinetic energy spectrometry analyses. Fragmentation of protonated peptides occurring in the second field-free region inside and outside the collision cell were distinguished by floating the collision cell above ground potential. Mass filtering using Q confirmed the mass assignments. The data indicate that product ions resulting from spontaneous decomposition are in some instances quantitatively more significant than those resulting from high-energy collisional activation. Furthermore, the differentiation of the products of low- and high-energy processes should facilitate spectral interpretation.

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mentioning

confidence: 99%

Determination of high-energy fragmentation of protonated peptides using a beqq hybrid mass spectrometer

Burlet

Orkiszewski

Gaskell

1993

J. Am. Soc. Mass Spectrom.

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“…As for the ground potential case the apparent m/z values produced by the time‐to‐mass calibration had to be multiplied by the ratio of m/z values of the two precursors (equal to the voltage ratio supplied by the peak matching circuit). This approach also turned out to be successful (Boyd et al, ), and the predicted increase of transmission efficiency for low‐ m/z product ions was indeed observed. Using this method we also demonstrated successful sequencing of a standard sample of physalaemin, a bioactive undecapeptide with an amide C‐terminus and a pyroglutamate (pGlu) residue at the N‐terminus.…”

Section: A Southern Interlude: Four Sectors Not Two For Peptide Sequementioning

confidence: 91%

From physical chemistry to mass spectrometry to government lab manager in half a century

Boyd

2014

Mass Spectrometry Reviews

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Fast atom bombardment/tandem mass spectrometry of physalaemin-like peptides

Harvan

Hass

Wilson

et al. 1987

Biol. Mass Spectrom.

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Fragment ion spectra obtained from collision-induced decomposition of protonated molecular ions have been used to determine amino acid sequences of several physalaemin-like peptides which were recently purified from rabbit stomach. This technique was chosen because the peptides were available in microgram quantities and were anticipated to contain pyroglutamate as the blocked N-terminal residue. Such spectra of several synthetic analogs of the naturally occurring peptides were obtained and analyzed to confirm the veracity of this peptide sequencing strategy. In addition, methyl ester derivatives of these synthetic peptides provided a crucial test for the spectral interpretation via the mass shifts thus induced.

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Computer-based mass measurement of fragment ion spectra from tandem magnetic sector mass spectrometers with an electrically floated collision cell

Cited by 11 publications

References 23 publications

Determination of high-energy fragmentation of protonated peptides using a beqq hybrid mass spectrometer

Determination of high-energy fragmentation of protonated peptides using a beqq hybrid mass spectrometer

From physical chemistry to mass spectrometry to government lab manager in half a century

Fast atom bombardment/tandem mass spectrometry of physalaemin-like peptides

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