Computer-assisted sperm analysis and reproductive science; a gift for understanding gamete biology from multidisciplinary perspectives

Holt, William V.; Cummins, James M.; Soler, Carles

doi:10.1071/rdv30n6_fo

Cited by 19 publications

(10 citation statements)

References 29 publications

(25 reference statements)

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“…This incompatibility of results can be explained by the type of motility analysis. The CASA-Mot (Holt, Cummins, & Soler, 2018;Soler, Cooper, Valverde, & Yániz, 2016) system ignores most of the cells with no real displacement, while in the subjective analysis the movement of the spermatozoa tail can be included in the oscillatory motile cells. Therefore, before comparing results, it is important to consider the existence of different concepts of motile spermatozoa depending on the approach used for motility analysis (subjective or objective).…”

Section: Discussionmentioning

confidence: 99%

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Meza

Caldeira

Valverde

et al. 2018

Reprod Domestic Animals

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Semen analysis is a key factor when determining the fertility ability in males. South American camelids, and in particular the alpaca, have been studied very little when compared with other farm animals. The aim of this work was to perform the kinematic characterization of alpaca spermatozoa collected directly from the deferent duct by using CASA-Mot (Computer Assisted Semen Analysis for Motility) technology. Samples were obtained every three days throughout the reproductive season during two periods and with a break of seven days in the middle. During both periods, the quality of the sample's motility and kinematics increased over the first two days and then subsequently decreased. This pattern was similar in all animals. It was concluded that the introduction of resting times can be useful to improve sperm quality for artificial insemination purposes in natural conditions.

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Section: Discussionmentioning

confidence: 99%

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Meza

Caldeira

Valverde

et al. 2018

Reprod Domestic Animals

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“…The introduction of computer-aided sperm analysis (CASA) dates back to the 1980s. It was developed in the academic community (Holt et al ., 1985) and progressed rapidly by commercial entities [for reviews, see Holt et al . (2018) and the associated special issue].…”

Section: Introductionmentioning

confidence: 99%

Rapid sperm capture: high-throughput flagellar waveform analysis

et al. 2019

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STUDY QUESTION Can flagellar analyses be scaled up to provide automated tracking of motile sperm, and does knowledge of the flagellar waveform provide new insight not provided by routine head tracking? SUMMARY ANSWER High-throughput flagellar waveform tracking and analysis enable measurement of experimentally intractable quantities such as energy dissipation, disturbance of the surrounding medium and viscous stresses, which are not possible by tracking the sperm head alone. WHAT IS KNOWN ALREADY The clinical gold standard for sperm motility analysis comprises a manual analysis by a trained professional, with existing automated sperm diagnostics [computer-aided sperm analysis (CASA)] relying on tracking the sperm head and extrapolating measures. It is not currently possible with either of these approaches to track the sperm flagellar waveform for large numbers of cells in order to unlock the potential wealth of information enclosed within. STUDY DESIGN, SIZE, DURATION The software tool in this manuscript has been developed to enable high-throughput, repeatable, accurate and verifiable analysis of the sperm flagellar beat. PARTICIPANTS/MATERIALS, SETTING, METHODS Using the software tool [Flagellar Analysis and Sperm Tracking (FAST)] described in this manuscript, we have analysed 176 experimental microscopy videos and have tracked the head and flagellum of 205 progressive cells in diluted semen (DSM), 119 progressive cells in a high-viscosity medium (HVM) and 42 stuck cells in a low-viscosity medium. Unscreened donors were recruited at Birmingham Women’s and Children’s NHS Foundation Trust after giving informed consent. MAIN RESULTS AND THE ROLE OF CHANCE We describe fully automated tracking and analysis of flagellar movement for large cell numbers. The analysis is demonstrated on freely motile cells in low- and high-viscosity fluids and validated on published data of tethered cells undergoing pharmacological hyperactivation. Direct analysis of the flagellar beat reveals that the CASA measure ‘beat cross frequency’ does not measure beat frequency; attempting to fit a straight line between the two measures gives \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}${\mathrm{R}}^2$\end{document} values of 0.042 and 0.00054 for cells in DSM and HVM, respectively. A new measurement, track centroid speed, is validated as an accurate differentiator of progressive motility. Coupled with fluid mechanics codes, waveform data enable extraction of experimentally intractable quantities such as energy dissipation, disturbance of the surrounding medium and viscous stresses. We provide a powerful and accessible research tool, enabling connection ...

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“…An additional possibility is that differences between subpopulations may relate to variation in an individual's strategy and that a strict genetic basis for this trait must be viewed with caution. More work is needed to understand the meaning of these results, but it could be hypothesized that a combination of genetic and external factors may be responsible for the distribution of spermatozoa in different subpopulations of an individual [60][61][62][63][64]. Although there are differences between animals, important questions still arise with regards to whether the proportions of spermatozoa in each subpopulation remain constant over time or if they vary with time in a given male.…”

Section: Plos Onementioning

confidence: 99%

Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

et al. 2021

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There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P < 0.05). Principal component (PC) analysis revealed that kinematic variables grouped into three components: PC1, related to velocity; PC2 to progressiveness and PC3 to oscillation. Subpopulation structure analysis identified four groups (P < 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, and 31.3% of the total sperm population. Males differed in the proportion of sperm in each of the kinematic subpopulations. This new approach for the analysis of reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species.

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Computer-assisted sperm analysis and reproductive science; a gift for understanding gamete biology from multidisciplinary perspectives

Cited by 19 publications

References 29 publications

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Rapid sperm capture: high-throughput flagellar waveform analysis

Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

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