Computer-assisted semen analysis systems: comparison with routine evaluation and prognostic value in male fertility and assisted reproduction

Krause, Walter

doi:10.1093/humrep/10.suppl_1.60

Cited by 98 publications

(66 citation statements)

References 9 publications

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“…Some studies have attempted to relate motion parameters as well as in vivo fertility and IVF in both humans and animals using average value and parametric statistical approach (Holt et al 1985, 1997, Marshburn et al 1992, Barratt et al 1993, Krause 1995, MacLeod & Irvine 1995. However, this approach considers a normal distribution of all variables, while semen is made up of billions of individual spermatozoa that make this regular distribution unlikely.…”

Section: Discussionmentioning

confidence: 99%

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

et al. 2012

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This study was designed for the identification of different sperm kinetic subpopulations in feline semen using artificial neural networks (ANNs) and for the evaluation of the effect of ejaculation on motility patterns of these subpopulations. Seven tomcats presented for routine orchiectomy were electroejaculated, and after 5 days, orchiectomized and epididymal tail sperms were collected. Sperm motility characteristics were evaluated using a computer-assisted sperm analyzer that provided individual kinetic characteristics of each spermatozoon. A total of 23 400 spermatozoa for electroejaculated and 9200 for epididymal tail samples were evaluated using a multivariate approach, comprising principal component analysis and ANN classification. The multivariate approach allowed the identification and characterization of three different and well-defined sperm subpopulations. There were significant differences before (epididymal tail spermatozoa) and after (electroejaculated sperm) ejaculation in sperm kinetic subpopulation characteristics. In both epididymal and ejaculated samples, the majority of subpopulation was characterized by high velocity and progressiveness; however, the electroejaculated samples showed significantly higher values, suggesting that the microenvironment of the epididymal tail could affect the sperm motility or, alternatively, seminal plasma could increase the kinetic characteristics of the spermatozoa, indicating that only after ejaculation, the spermatozoa express their motility potential. Nevertheless, further studies are required to clarify the functional significance of each kinetic subpopulation.

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Section: Discussionmentioning

confidence: 99%

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

et al. 2012

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“…Subsequently several objective methods have been developed to measure sperm horizontal movement, the CASA being the most widely used one (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). Although CASA gives multiple sperm motility parameters, their correlation with fertility potential of spermatozoa is not well defined (23)(24)(25)(26)(27). Some workers have analyzed sperm cells showing upward movement against the gravity by spectrophotometric methods (28,29).…”

Section: Discussionmentioning

confidence: 99%

“…But, all the available techniques consider only the ''horizontal'' velocity and there is not a single instrument available for measuring the ''vertical'' velocity of spermatozoa. The sophisticated computer aided semen analyzer (CASA) based on microscopic video photographic method is widely used for estimating sperm horizontal velocity from the horizontal plane of a glass slide or haemocytometer or Makler Chamber (23)(24)(25)(26)(27). Another objective method of sperm motility analysis is the spectrophotometric/turbidimetric method described by Sokoloski et al (28) and simplified and made user-friendly by Majumder and Chakrabarti (29).…”

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confidence: 99%

A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: A novel concept

et al. 2007

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Background:The presently available cell motility‐analyzers measure primarily the “horizontal” velocity and there is no instrument available for “vertical” velocity measurement. This development was based on the turbidimetric method of sperm motility analysis.Methods:Sperm was layered at the bottom of the cuvette containing buffer solution and exposed to the spectrophotometric light path at different heights to track the vertically moving sperms. The vertical movement was materialized with the development of an electromechanical up–down movement devise for the cuvette accomplished with the help of a cuvette holder‐stepper motor‐computer assembly. The entire system was controlled by the necessary motion control, data acquisition, and data processing softwares developed for cuvette movement and data analysis.Results:Using goat sperm as the model a unique computer‐based spectrophotometric system has been developed for the first time to determine the average “vertical” velocity of motile cells.Conclusions:Undertaking upward movement against gravity is much tougher as compared with horizontal movement. Consequently average vertical velocity is expected to be a much better identifying parameter for assessing semen and other motile cell quality. The novel instrumental system developed by us has thus the potential for immense application in human infertility clinics, animal‐breeding centres, centres for conservation of endangered species, and also for research work on vertical velocity of spermatozoa and other motile cells, such as bacteria, protozoa, etc. © 2007 International Society for Analytical Cytology

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“…Evaluation of sperm motility represents an important parameter because it is significantly correlated with the total number of offspring born, for example, fertility (Barnett et al, 1993;Krause, 1995;Parkinson, 2009). It is an accepted fact that normal ejaculates do contain a small percentage of spermatozoa that are abnormal in morphology.…”

Section: Discussionmentioning

confidence: 99%

Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits

Kovács

Tornyos

Matics

et al. 2011

Animal

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Pannon White (n 5 12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n 5 12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n 5 10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropinreleasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27% compared to control, P , 0.05) and remained lower ( P , 0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88% on days 17 and 29 compared to controls ( P , 0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65% to 53% in the semen samples of toxin-treated animals compared to controls ( P . 0.05). The ratio of spermatozoa with abnormal morphology increased ( P , 0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma ( P , 0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45% compared to control ( P , 0.01) and resulted in lower ( P , 0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration -its effect needs further examination.

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Computer-assisted semen analysis systems: comparison with routine evaluation and prognostic value in male fertility and assisted reproduction

Cited by 98 publications

References 9 publications

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: A novel concept

Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits

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