Computer‐assisted motion analysis of sperm from the common carp

Toth, Gregory P.; Christ, S. A.; McCarthy, H. W.; Torsella, Joni; Smith, Milton T.

doi:10.1111/j.1095-8649.1995.tb06023.x

Cited by 35 publications

(39 citation statements)

References 30 publications

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“…Also Campos-Mendoza et al (2004) noted that variation in day length during spawning period affected the sperm volume in males. Seminal plasma not only maintains immobility of spermatozoa but also protects them (Cosson et al, 1997); this is the case, for example, in the common carp Cyprinus carpio (Toth et al, 1995) and in the rainbow trout Oncorhynchus mykiss (Billard, 1986). The ionic composition may change during the spawning season .…”

Section: Discussionmentioning

confidence: 99%

Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration

Golpour¹,

Akhoundian²,

Khara³

et al. 2013

CZECH J ANIM SCI

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ABSTRACT:In this study, changes of pH, ionic (Na + , K + , Ca 2+ , and Mg 2+ ), biochemical (total protein, glucose, and cholesterol) compositions of seminal plasma, sperm motility traits (percentage of motile spermatozoa and sperm movement duration), and sperm production characteristics (sperm volume, spermatocrit, and sperm density) were studied in Caspian roach, Rutilus rutilus caspicus, during spawning migration. Sperm of 10 males was collected three times during the spawning migration (in February, March, and April). The results showed that sperm motility parameters (percentage of motile spermatozoa and sperm movement duration) changed significantly (P < 0.05) during the reproductive season, but sperm density, spermatocrit, and sperm volume did not show significant differences during spawning migration. Analyses performed at each sampling time (February, March, and April) showed significant differences (P < 0.05) in calcium, magnesium, potassium, and cholesterol, whereas there were no significant changes in Na + , pH, total protein, glucose, and cholesterol.

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Section: Discussionmentioning

confidence: 99%

Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration

Golpour¹,

Akhoundian²,

Khara³

et al. 2013

CZECH J ANIM SCI

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“…In common carp males, the application of Ovopel (1 pellet kg -1 ) enables semen to be obtained even after 72 h, but the semen volume and sperm number at this time are significantly lower than values noted after 24 h (Cejko et al 2011). This indicates that in common carp males, latency time is rather short and semen should be taken no longer than 24 h after hormonal treatment (Toth et al 1995;Christ et al 1996;Cejko et al 2011). The presented study indicated that in comparison to the first Ovopel treatment, the repeated stimulation of males with this preparation resulted in decreased semen volume and total number of sperm.…”

Section: Discussionmentioning

confidence: 99%

Sperm quality and selected biochemical markers of seminal plasma at the beginning of the reproductive period of common carp, Cyprinus carpio L.

et al. 2013

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Changes in semen quality and selected biochemical markers were analyzed during a week of spawning season of common carp Cyprinus carpio L. Semen was obtained twice, on May 30 and on June 7, and each time it was collected 24 h after hormonal stimulation using Ovopel [(D-Ala 6 , Pro 9 NEt)-mGnRH ? metoclopramide] in 1 pellet kg -1 . The total volume of semen (ml), volume of semen per kg of body weight (ml kg -1 b.w.), sperm concentration (910 9 ml -1 ), total number of sperm per kg of body weight (910 9 kg -1 b.w.), pH of semen, pH of seminal plasma, seminal plasma osmotic pressure (mOsm kg -1 ) and the total protein content in seminal plasma (mg ml -1 ) were determined. A 10 mM Tris buffer containing 100 mM NaCl with 0.5 % BSA (pH 9.0, osmolality 200 mOsm kg ), movement linearity (LIN, %), wobbling index (WOB, %), amplitude of lateral head displacement (ALH, lm) and beat cross-frequency (BCF, Hz). The volume of semen per kg of BW, total number of sperm per kg of BW and semen pH were significantly lower at the second semen sampling compared to the first semen sampling. Volume of semen at the second sampling correlated positively with CASA parameters. A lack of differences among CASA parameters between both collection periods indicates good quality of carp sperm hormonally stimulated with Ovopel twice at a 1-week interval.

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“…Once diluted, milt was held on ice in a cooler between preparation of slides. Other investigators of sperm motility have reported sample preparation periods to post-activation of 0 sec (dilution prepared on microscope's stage) (Vladic and Jaervi, 1997) and 15 sec (Toth et al, 1995). It is important to maintain consistency in post-activation times among samples.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Approximately 100 spermatozoa should be tracked per sample and it is a time-consuming process. Computerassisted sperm analysis (CASA) to assess the effects of pollutants on fish sperm has been successfully employed to estimate sperm swimming speed (Toth et al, 1995). This device automatically assesses speeds of high numbers of sperm and provides a digital data output (Kime et al, 1996).…”

Section: Sperm Motilitymentioning

confidence: 99%

Estimation of Male Reproductive Success of Marine Fisheries

Trippel¹

2003

J. Northw. Atl. Fish. Sci.

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The subject of reproductive potential of fish populations is dominated by studies on the female gender. Studies on male reproduction are relatively few, but have increased in number during the previous decade. The objectives of this contribution were to describe and quantify the reproductive traits that make up the viable sperm production of a population. Some of these reproductive traits were easily measured from wild fish (e.g., mature testes weight), and others were more easily measured on captive fish (e.g., fertilization potential and sperm motility). Results of laboratory and field studies were then integrated to generate estimates of viable sperm production of a fish stock. A number of experimental protocols have been employed over the years to assess male fertility. The strengths and weaknesses of the different experimental approaches were reviewed and appropriate recommendations given towards establishing standardized protocols. Although the review is broad in nature, and includes references to a number of marine fishes, it concentrated on exploited species that occur in the North Atlantic and Baltic Sea within the taxonomic groups gadoidae, pleuronectoidae and clupeidae (Clupeiformes). In addition, published data on male reproductive traits of species in these taxa were tabulated and summarized. The terms of interest included sex ratio, maturity state, testes weight, sperm fertilization potential (artificial fertilization and paired mating), sperm density, sperm motility and paternal effects on early life history traits.An equation for determinate spawners was described that quantifies a population's viable sperm production using data on adult body characteristics and associated quantity and quality of sperm. The equation was used to establish time series of viable sperm production for Atlantic cod (Gadus morhua) of Newfoundland and Labrador. Results indicated on average 1 600 billion viable sperm were released to produce one recruit at age 3 years but with substantial variation among years in relation to changes in stock composition and growth as well as stock size. Distinct peaks in the number of viable sperm in 1981 and 1986 corresponded to distinct peaks in the number of age 3 recruits in those same years.

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Computer‐assisted motion analysis of sperm from the common carp

Cited by 35 publications

References 30 publications

Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration

Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration

Sperm quality and selected biochemical markers of seminal plasma at the beginning of the reproductive period of common carp, Cyprinus carpio L.

Estimation of Male Reproductive Success of Marine Fisheries

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