Purpose-To create a thermal map of ambient air, radiant, and evaporative temperatures and humidity throughout the NICU nursery by season across a calendar year.Subjects-Each of the 32 bed cubicles distributed in five rooms in a Level III nursery was measured.Methods-Temperatures were recorded at a consistent time on one day during January, April, July, and October.Main outcome measures-An electronic monitor (QUESTemp° 34, Quest Technologies, Oconomowoc, WI) was used to measure dry bulb, wet bulb, and globe thermometer temperatures. Results-ANOVA revealed statistically significant (p < .000) differences in season, room, and season by room interaction. Room ambient air temperatures differed < 2 °F across season. Radiant temperature paralleled air temperature. Humidity, the predominant difference across season, produced evaporative temperatures considerably lower than room air temperature, and the gradient between mean nursery dry bulb and wet bulb temperature was 9.3 °F in summer and 16.8 °F in winter.Conclusions-The thermal map revealed seasonal thermal differences, particularly in humidity level and evaporative temperature. Room temperature alone does not reflect the total thermal environment. Recommendations include periodic assessment of nurseries including air, evaporative, and radiant temperatures as well as humidity to more fully appreciate the impact of the thermal environment on infants.