Computer-aided microfluidics (CAMF): from digital 3D-CAD models to physical structures within a day

Waldbaur, Ansgar; Carneiro, Bernardo; Hettich, Paul; Wilhelm, Elisabeth; Rapp, Bastian E.

doi:10.1007/s10404-013-1177-x

Cited by 37 publications

(25 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A custom-made maskless lithography system based on a commercially available digital mirror device with 1024 × 768 individually addressable pixels and different optical components was used and has been previously described [15].…”

Section: Methodsmentioning

confidence: 99%

Functionalization of paper using photobleaching: A fast and convenient method for creating paper‐based assays with colorimetric and fluorescent readout

Nargang

Runck

Helmer

et al. 2016

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

Lateral flow immunoassays (LFIA), where a protein–protein interaction can be monitored on a test strip by a color reaction, are of high interest in the field of point‐of‐care diagnostics due to their cost‐efficient production, portability, and ease of use. Despite their simple appearance, state‐of‐the‐art manufacturing of such test strips is not trivial since the strips comprise of several reaction zones: cellulose serves as hydrophilic support with excellent flow properties; and nitrocellulose is the membrane of choice for zones with immobilized biomolecules due to its hydrophobicity and thus preferable adhesion properties. These individual reaction zone membranes need to be joined together after fabrication. Variations in general membrane properties and production‐related lot‐to‐lot variations make it difficult to produce reliable tests with high reproducibility. In this paper, we present a facile and rapid method to immobilize antibodies directly onto cellulose by using maskless projection lithography. With this method it is possible to manufacture LFIA strips with individual reaction zones in a single material, i.e. cellulose.

show abstract

Section: Methodsmentioning

confidence: 99%

Functionalization of paper using photobleaching: A fast and convenient method for creating paper‐based assays with colorimetric and fluorescent readout

Nargang

Runck

Helmer

et al. 2016

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Using the benchtop SL system we were able to create structures with a resolution of a few 100 μm. Using a custom‐built lithography system based on a digital mirror device which we have previously described we achieved resolutions fully compatible with the demands of microfluidics and microoptics . By consecutively illuminating several high resolution images next to each other (a process called stitching) we achieved a resolution of 200 μm on a centimeter area as can be seen from the Tesla Mixer in Figure A.…”

Section: Time Required For the Fabrication Of A Microfluidic Chip Usimentioning

confidence: 97%

Liquid PMMA: A High Resolution Polymethylmethacrylate Negative Photoresist as Enabling Material for Direct Printing of Microfluidic Chips

et al. 2017

Self Cite

View full text Add to dashboard Cite

Polymethymethacrylate (PMMA) is one of the most important thermoplasts and a commonly used material in microsystem fabrication, for example, microfluidics owning mainly to its optical transparency, biocompatibility, low autofluorescence, and low cost. However, being a thermoplastic material PMMA is typically structured using industrial replication techniques making PMMA unsuitable for rapid prototyping. The fact that neither material nor processing technique can be directly transferred from laboratory to industrial state makes the research‐to‐business conversion often extremely difficult in microfluidics since material properties have a major impact on the final system behavior. This paper presents “Liquid PMMA,” a fast curing viscous PMMA prepolymer which can be used as a negative photoresist and directly structured using ultraviolet or visible light with tens of micron resolution and smooth surfaces. Using this technique microfluidic chips in PMMA can be fabricated within minutes. The cured Liquid PMMA parts show the same high optical transparency, low autofluorescence, and surface properties like commercial PMMA. In this way, microfluidic chips can be rapidly developed and optimized on the laboratory scale in the same material which is later on used on the industrial scale.

show abstract

“…The pattern to be generated is projected onto the samples using a digital mirror device (DMD). By adjusting light intensity and duration of exposure it is possible to produce both, black-and-white patterns as well as grayscale patterns [14].…”

Section: Lithographical Setupsmentioning

confidence: 99%

“…(b) For obtaining a higher resolution an advanced lithography setup for maskless projection lithography was used. This setup uses a digital mirror device as mask [14]. The light patterned by the digital mirror device is directed via a deflection mirror through the lens onto the substrate located on top of the setup.…”

Section: Materials Used For Protein Patterningmentioning

confidence: 99%

Protein assay structured on paper by using lithography

et al. 2015

View full text Add to dashboard Cite

There are two main challenges in producing a robust, paper-based analytical device. The first one is to create a hydrophobic barrier which unlike the commonly used wax barriers does not break if the paper is bent. The second one is the creation of the (bio-)specific sensing layer. For this proteins have to be immobilized without diminishing their activity. We solve both problems using light-based fabrication methods that enable fast, efficient manufacturing of paper-based analytical devices. The first technique relies on silanization by which we create a flexible hydrophobic barrier made of dimethoxydimethylsilane. The second technique demonstrated within this paper uses photobleaching to immobilize proteins by means of maskless projection lithography. Both techniques have been tested on a classical lithography setup using printed toner masks and on a lithography system for maskless lithography. Using these setups we could demonstrate that the proposed manufacturing techniques can be carried out at low costs. The resolution of the paper-based analytical devices obtained with static masks was lower due to the lower mask resolution. Better results were obtained using advanced lithography equipment. By doing so we demonstrated, that our technique enables fabrication of effective hydrophobic boundary layers with a thickness of only 342 µm. Furthermore we showed that flourescine-5-biotin can be immobilized on the non-structured paper and be employed for the detection of streptavidinalkaline phosphatase. By carrying out this assay on a paper-based analytical device which had been structured using the silanization technique we proofed biological compatibility of the suggested patterning technique.

show abstract

Computer-aided microfluidics (CAMF): from digital 3D-CAD models to physical structures within a day

Cited by 37 publications

References 22 publications

Functionalization of paper using photobleaching: A fast and convenient method for creating paper‐based assays with colorimetric and fluorescent readout

Functionalization of paper using photobleaching: A fast and convenient method for creating paper‐based assays with colorimetric and fluorescent readout

Liquid PMMA: A High Resolution Polymethylmethacrylate Negative Photoresist as Enabling Material for Direct Printing of Microfluidic Chips

Protein assay structured on paper by using lithography

Contact Info

Product

Resources

About