Computational study of an integrated microfluidic device for active separation of RBCs and cell lysis

Jalilvand, Elahe; Shamloo, Amir; Gangaraj, Mojtaba Hassani

doi:10.1016/j.cep.2022.108891

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…For example, microfluidic separation chips can be used to separate trace circulating tumor cells in the human peripheral blood for disease diagnosis [ 8 ]. The microfluidic separation technology is mainly divided into two categories, namely, passive separation [ 9 , 10 , 11 ] and active separation [ 12 , 13 , 14 ]. The former is usually separated by designing different channel geometries to make particles reach different positions at high speeds [ 15 ], which has the advantages of high flux, simple design, and low cost.…”

Section: Introductionmentioning

confidence: 99%

A Perturbed Asymmetrical Y-TypeSheathless Chip for Particle Control Based on Adjustable Tilted-Angle Traveling Surface Acoustic Waves (ataTSAWs)

Duan

Zhang

2022

Biosensors

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The precise control of target particles (20 µm) at different inclination angles θiis achieved by combining a perturbed asymmetric sheathless Y-type microchannel and a digital transducer (IDT). The offset single-row micropillar array with the buffer area can not only concentrate large and small particles in a fixed region of the flow channel, but also avoid the large deflection of some small particles at the end of the array. The addition of the buffer area can effectively improve the separation purity of the chip. By exploring the manufacturing process of the microchannel substrate, an adjustable tilted-angle scheme is proposed. The use of ataTSAW makes the acoustic field area in the microchannel have no corner effect region. Through experiments, when the signal source frequency was 33.6 MHz, and the flow rate was 20 µL/min, our designed chip could capture 20 µm particles when θi= 5°. The deflection of 20 µm particles can be realized when θi= 15°–45°. The precise dynamic separation of 20 µm particles can be achieved when θi= 25°–45°, and the separation purity and efficiency were 97% and 100%, respectively.

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Section: Introductionmentioning

confidence: 99%

A Perturbed Asymmetrical Y-TypeSheathless Chip for Particle Control Based on Adjustable Tilted-Angle Traveling Surface Acoustic Waves (ataTSAWs)

Duan

Zhang

2022

Biosensors

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“…The main reason for this difference is the higher surface-to-volume ratio in microscales. 1 The properties of fluids in microscales make them suitable for various biological applications such as cell analysis, 2 cell separation, 3 cell lysis, 4,5 DNA extraction 6 and purification, 4,7 vaccine development, 8 cell culture 9 and co-culture, 10 and organ on a chip. 11,12 Microfluidic systems can be implemented on different platforms 13 such as paper-based microfluidics, 14 large-scale integration (LSI), 15 centrifugal microfluidics, 16 electrowetting on dielectric (EWOD) microfluidics, 17 and droplet microfluidics.…”

Section: Introductionmentioning

confidence: 99%

“…The main reason for this difference is the higher surface-to-volume ratio in microscales . The properties of fluids in microscales make them suitable for various biological applications such as cell analysis, cell separation, cell lysis, , DNA extraction and purification, , vaccine development, cell culture and co-culture, and organ on a chip. , …”

Section: Introductionmentioning

confidence: 99%

Developing an Off-the-Shelf Microfluidic Droplet Generation Device for Cell Encapsulation

Hassani-Gangaraj

Shamloo

2022

Ind. Eng. Chem. Res.

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Droplet microfluidics is a platform of microfluidics in which two immiscible fluids are used to generate droplets for various biomedical applications. This platform introduces several advantages in applications such as cell lysis, cell culture, co-culture, and cell encapsulation. The most important issues regarding droplet generation devices are the fabrication complexity and maintenance of these devices. In this study, a simple and easy-tofabricate microdroplet generator is designed and fabricated to resolve these issues. Furthermore, since this device is easy to fabricate and use, it can play a key role in the fabrication of medical devices for controlling infectious diseases in poor and underdeveloped countries where 3D-printed and glass capillary devices have high final costs. The introduced droplet generator is an independent unit that is able to attach to any microfluidic chip and inject microdroplets into the device. This droplet generator is able to generate homogeneous droplets of various diameters, ranging from 50 to 2000 μm. This device is also efficient for single-and multi-cell encapsulation. When using a cell solution containing MCF-7 cells with a concentration of 100 cell/μL, 45.7% of droplets contain no cells, 49.3% of droplets contain one, two, or three cells, and 5% contain four or more cells. These results are perfectly in agreement with the results obtained from the Poisson distribution for cell encapsulation.

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Passive droplet sorting engendered by emulsion flow in constricted and parallel microchannels

Bhattacharjee

Chakraborty

Atta

2022

Chemical Engineering and Processing - Process Intensification

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Computational study of an integrated microfluidic device for active separation of RBCs and cell lysis

Cited by 6 publications

References 55 publications

A Perturbed Asymmetrical Y-TypeSheathless Chip for Particle Control Based on Adjustable Tilted-Angle Traveling Surface Acoustic Waves (ataTSAWs)

A Perturbed Asymmetrical Y-TypeSheathless Chip for Particle Control Based on Adjustable Tilted-Angle Traveling Surface Acoustic Waves (ataTSAWs)

Developing an Off-the-Shelf Microfluidic Droplet Generation Device for Cell Encapsulation

Passive droplet sorting engendered by emulsion flow in constricted and parallel microchannels

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