Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation

Li, Rui; Sun, Wei; Liu, Chun-Zhao

doi:10.1002/btpr.682

Cited by 9 publications

(5 citation statements)

References 35 publications

(57 reference statements)

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“…Wang and Wang [12] investigated mass transfer in bubble column with the coupled CFD-PBM model and showed that the CFD-PBM coupled model could predict effectively the hydrodynamics and mass transfer. Liu et al [13] performed CFD-PBM coupled simulations of airlift bioreactor for hairy root culture and presented good agreement between simulations and experiments in terms of bubble number density. Xing et al [14] investigated the ability of the CFD-PBM coupled model to account for the effect of viscosity on the gas holdup in a bubble column.…”

Section: Introductionmentioning

confidence: 88%

“…PBM is an effective method to calculate BSD for gas-liquid flows on the condition that bubble coalescence and breakup models are provided as model closures. Many researchers have used the coupled CFD-PBM model to predict the hydrodynamics and mass transfer in multiphase reactors [9][10][11][12][13][14]. Their studies showed that a better agreement between simulations and experiments was obtained when BSD was considered compared to using a constant mean bubble diameter.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

CFD-PBM Coupled Simulation of an Airlift Reactor with Non-Newtonian Fluid

Han

Sha

Лаари

et al. 2017

Oil Gas Sci. Technol. – Rev. IFP Energies nouvelles

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-Hydrodynamics of an AirLift Reactor (ALR) with tap water and non-Newtonian fluid was studied experimentally and by numerical simulations. The Population Balance Model (PBM) with multiple breakup and coalescence mechanisms was used to describe bubble size characteristics in the ALR. The interphase forces for closing the two-fluid model were formulated by considering the effect of Bubble Size Distribution (BSD). The BSD in the ALR obtained from the coupled Computational Fluid Dynamics (CFD)-PBM model was validated against results from digital imaging measurements. The simulated velocity fields of both the gas and liquid phases were compared to measured fields obtained with Particle Image Velocimetry (PIV). The simulated results show different velocity field profile features at the top of the ALR between tap water and nonNewtonian fluid, which are in agreement with experiments. In addition, good agreement between simulations and experiments was obtained in terms of overall gas holdup and bubble Sauter mean diameter. NOMENCLATURE SymbolsVolume fraction of gas/liquid phase, 1 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

CFD-PBM Coupled Simulation of an Airlift Reactor with Non-Newtonian Fluid

Han

Sha

Лаари

et al. 2017

Oil Gas Sci. Technol. – Rev. IFP Energies nouvelles

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“…CFD implementations and their simulations are a numerical tool to solutions of Navier–Stokes equations describing fluid characteristics, dynamics, and turbulences in a bioreactor. Kaiser et al presented numerical simulations of fluids in wave bag bioreactors and Liu et al established a comprehensive 2D axisymmetric CFD model to investigate hydrodynamics, oxygen distribution, and mass transfer in an ALR using hairy roots of Echinacea purpurea for experimental comparison. During cultivation several process parameters mostly properties of the cultivation medium have to be monitored continuously.…”

Section: Process Control Management and Modelingmentioning

confidence: 99%

Bioprocessing of differentiated plant in vitro systems

Steingroewer

Bley

Georgiev

et al. 2012

Engineering in Life Sciences

122

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Plant cells contain a wide range of interesting secondary metabolites, which are used as natural pigments and flavoring agents in foods and cosmetics as well as phyto‐pharmaceutical products. However, conventional industrial extraction from whole plants or parts of them is limited due to environmental and geographical issues. The production of secondary metabolites from in vitro cultures can be considered as alternative to classical technologies and allows a year‐round cultivation in the bioreactor under optimal conditions with constant high‐level quality and quantity. Compared to plant cell suspensions, differentiated plant in vitro systems offer the advantage that they are genetically stable. Moreover, the separation of the biomass from culture medium after fermentation is much easier. Nevertheless, several investigations in the literature described that differentiated plant in vitro systems are instable concerning the yield of the target metabolites, especially in submerged cultivations. Other major problems are associated with the challenges of cultivation conditions and bioreactor design as well as upscaling of the process. This article reviews bioreactor designs for cultivation of differentiated plant in vitro systems, secondary metabolite production in different bioreactor systems as well as aspects of process control, management, and modeling and gives perspectives for future cultivation methods.

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“…A continuous flow of research can be observed in the past few years, which reveals the development of several mathematical models that efficiently describe the nutrient uptake, mass transfer, and gas distribution in different layers and their effect on tissue growth (Mairet et al 2010;Liu et al 2011;Palavalli et al 2012). As known, the tissue attains a nonlinear growth pattern, and one cannot understand the process by focusing only on a single or couple of growth parameters on any one scale.…”

Section: Growth Simulations and Modelingmentioning

confidence: 99%

“…With this view, nowadays, bioreactor technology utilizes engineering principles and mathematical formulations for mass production. Various research groups are working on issues like optimization of physical, biological, and chemical culture conditions (Prakash et al 2010;Mehrotra et al 2013a;Stiles and Liu 2013), offline and/or online measurement of growth (Uozumi 2004), mass transfer behavior (Liu et al 2011), synergistic effects of various physical and chemical parameters on growth, downstream processing (intracellular/extracellular), and product recovery (Bhagyalakshmi and Thimmaraju 2009;2012) in the course and/or at the end of scale-up. This may fairly help in filling the gap between capital cost and the benefits of technology at industrial scale (Stiles and Liu 2013).…”

Section: Bioreactor Technologymentioning

confidence: 99%

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

et al. 2015

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Agrobacterium rhizogenes-mediated hairy roots (HR) were developed in the laboratory to mimic the natural phenomenon of bacterial gene transfer and occurrence of disease syndrome. The timeline analysis revealed that during 90 s, the research expanded to the hairy root-based secondary metabolite production and different yield enhancement strategies like media optimization, up-scaling, metabolic engineering etc. An outlook indicates that much emphasis has been given to the strategies that are helpful in making this technology more practical in terms of high productivity at low cost. However, a sequential analysis of literature shows that this technique is upgraded to a biotechnology platform where different intra- and interdisciplinary work areas were established, progressed, and diverged to provide scientific benefits of various hairy root-based applications like phytoremediation, molecular farming, biotransformation, etc. In the present scenario, this biotechnology research platform includes (a) elemental research like hairy root-mediated secondary metabolite production coupled with productivity enhancement strategies and (b) HR-based functional research. The latter comprised of hairy root-based applied aspects such as generation of agro-economical traits in plants, production of high value as well as less hazardous molecules through biotransformation/farming and remediation, respectively. This review presents an indicative timeline portrayal of hairy root research reflected by a chronology of research outputs. The timeline also reveals a progressive trend in the state-of-art global advances in hairy root biotechnology. Furthermore, the review also discusses ideas to explore missing links and to deal with the challenges in future progression and prospects of research in all related fields of this important area of plant biotechnology.

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Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation

Cited by 9 publications

References 35 publications

CFD-PBM Coupled Simulation of an Airlift Reactor with Non-Newtonian Fluid

CFD-PBM Coupled Simulation of an Airlift Reactor with Non-Newtonian Fluid

Bioprocessing of differentiated plant in vitro systems

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

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