Bioprocessing of differentiated plant in vitro systems

Steingroewer, Juliane; Bley, Thomas; Georgiev, Vasil; Ivanov, Ivan; Lenk, Felix; Marchev, Andrey S.; Pavlov, Atanas

doi:10.1002/elsc.201100226

Cited by 122 publications

(85 citation statements)

References 125 publications

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“…However, good results could probably also be achieved using temporary immersion systems which provided sufficient culture aeration and periodical medium mixing during the immersion phase (Steingroewer et al 2013). …”

Section: Discussionmentioning

confidence: 99%

Accumulation of dibenzocyclooctadiene lignans in agar cultures and in stationary and agitated liquid cultures of Schisandra chinensis (Turcz.) Baill

Szopa

Kokotkiewicz

Marzec-Wróblewska

et al. 2015

Appl Microbiol Biotechnol

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Schisandra chinensis plant in vitro cultures were maintained on Murashige and Skoog (MS) medium supplemented with 3 mg/l 6-benzyladenine (BA) and 1 mg/l 1-naphthaleneacetic acid (NAA) in an agar system and also in two different liquid systems: stationary and agitated. Liquid cultures were grown in batch (30 and 60 days) and fed-batch modes. In the methanolic extracts from lyophilized biomasses and in the media, quantification of fourteen dibenzocyclooctadiene lignans identified based on co-chromatography with authentic standards using high-performance liquid chromatography with diode array detection (HPLC-DAD) and/or liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) methods. For comparison purposes, phytochemical analyses were performed of lignans in the leaves and fruits of the parent plant. The main lignans detected in the biomass extracts from all the tested systems were schisandrin (max. 65.62 mg/100 g dry weight (DW)), angeloyl-/tigloylgomisin Q (max. 49.73 mg/100 g DW), deoxyschisandrin (max. 43.65 mg/100 g DW), and gomisin A (max. 34.36 mg/100 g DW). The highest total amounts of lignans in the two tested stationary systems were found in extracts from the biomass harvested after 30 days of batch cultivation: 237.86 mg/100 g DW and 274.65 mg/100 g DW, respectively. In the agitated culture, the total content reached a maximum value of 244.80 mg/100 g DW after 60 days of the fed-batch mode of cultivation. The lignans were not detected in the media. This is the first report which documents the potential usefulness of S. chinensis shoot cultures cultivated in liquid systems for practical purposes.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-015-7230-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Accumulation of dibenzocyclooctadiene lignans in agar cultures and in stationary and agitated liquid cultures of Schisandra chinensis (Turcz.) Baill

Szopa

Kokotkiewicz

Marzec-Wróblewska

et al. 2015

Appl Microbiol Biotechnol

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“…In vitro cultures for production of plant derived compounds are increasingly gaining industrial application, because of availability on demand, potential for technological process standardization, independence of seasonality, purity of the product and low environmental impact (Steingroewer et al 2013). Secoiridoid glycosides and xanthones have been reported as characteristic secondary metabolites in C. erythraea (Aberham et al 2011;Piatczak et al 2011;Stefkov et al 2014).…”

Section: Resultsmentioning

confidence: 99%

In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea

Boroduske

Nakurte

Tomsone

et al. 2016

Plant Cell Tiss Organ Cult

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Centaurium erythraea is medicinal plant with high content of secoiridoid glycosides and xanthones with gastroprotective, hepatoprotective and antioxidative properties. In vitro culture based C. erythraea production would offer sustainable and economically feasible alternative to wild plant harvest provided that comparable productivity could be achieved in artificially propagated plants. To identify most productive C. erythraea system the secoiridoid glycoside and xanthone production and profiles using liquid chromatography-mass spectrometry were assessed in in vitro shoot, callus and cell suspension culture-derived material in comparison with wild C. erythraea plants.In vitro shoot culture was the most productive system for C. erythraea secoiridoid production (total yield 86 mg g -1 dw) and cell culture was the most productive system for phenolic compound production (19 mg GAE g -1 dw). The effect of elicitors yeast extract, methyljasmonate (MeJA) and chitosan on secondary metabolite yield indicated that elicitation with MeJA and chitosan significantly decreased yield of sweroside and eustomin, respectively. Significant quantitative differences of major secoiridoids and xanthones indicated that further methodological improvements for increasing yield of major secondary metabolites of in vitro origin need to be explored for in vitro cultivation to become a feasible alternative to C. erythraea wild plant harvest.

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“…In vitro cultures possess numerous advantages, including biosynthesis of biosafe metabolites according to the good manufacturing practices (GMP) and independency of the environmental factors. Also 6 opportunities for facilitating the down-stream processes resulting in increased yields of the target metabolites through different biotechnological approaches are available (Steingroewer et al 2013;Marchev et al 2014;Grech-Baran et al 2015).…”

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confidence: 99%