Compromised Biomechanical Properties, Cell–Cell Adhesion and Nanotubes Communication in Cardiac Fibroblasts Carrying the Lamin A/C D192G Mutation

Lachaize, Véronique; Peña, Brisa; Ciubotaru, Catalin D.; Cojoc, Dan; Chen, Suet Nee; Taylor, Matthew R.G.; Mestroni, Luisa; Sbaizero, Orfeo

doi:10.3390/ijms22179193

Cited by 6 publications

(3 citation statements)

References 79 publications

(125 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a 2021 study, a pulsed optical tweezer was used to detect the viscosity parameters of wild-type and A350P lamin A, and it was found that the complex viscosity for A350P lamin A was higher than that of wild-type lamin A, which may translate into nuclear plasticity ( Mukherjee et al, 2021 ). Moreover, mutation of the lamin A/C gene D192G also led to weakened tunneling nanotubes (TNTs) and compromised cell–cell adhesion in neonatal rat ventricular fibroblasts, as shown by biomechanical studies using optical tweezers ( Lachaize et al, 2021 ). To date, the topic of lamin mechanics is still vastly unstudied, and this application of optical tweezers could definitely be extended to examine the mechanics of other mutant lamins, as well as any other intermediate filaments.…”

Section: Single-molecule Studies Of Cardiovascular Diseasesmentioning

confidence: 99%

Application of optical tweezers in cardiovascular research: More than just a measuring tool

Yang

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Recent advances in the field of optical tweezer technology have shown intriguing potential for applications in cardiovascular medicine, bringing this laboratory nanomechanical instrument into the spotlight of translational medicine. This article summarizes cardiovascular system findings generated using optical tweezers, including not only rigorous nanomechanical measurements but also multifunctional manipulation of biologically active molecules such as myosin and actin, of cells such as red blood cells and cardiomyocytes, of subcellular organelles, and of microvessels in vivo. The implications of these findings in the diagnosis and treatment of diseases, as well as potential perspectives that could also benefit from this tool, are also discussed.

show abstract

Section: Single-molecule Studies Of Cardiovascular Diseasesmentioning

confidence: 99%

Application of optical tweezers in cardiovascular research: More than just a measuring tool

Yang

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, cell adhesion plays a key role in health and disease: for example, the impairment of adhesiveness is typical in the pathogenesis of certain diseases, like ACM, where the speculated detachment of cardiomyocytes due to altered cell–cell junctions is thought to trigger the disorder. It has been previously reported by our group that neonal rat ventricula fibroblast (NRVF) carring LMNA D192G mutation present reduced cell–cell adhesion properties when compared with control (non-mutated NRVF [ 50 ]. Adhesive properties and events are usually derived from the retraction segment of a F-d curve.…”

Section: Force Spectroscopy With Afmmentioning

confidence: 99%

Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy

Peña

Adbel-Hafiz

Cavasin

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Arrhythmogenic cardiomyopathy (ACM) is an inherited heart muscle disorder characterized by progressive replacement of cardiomyocytes by fibrofatty tissue, ventricular dilatation, cardiac dysfunction, arrhythmias, and sudden cardiac death. Interest in molecular biomechanics for these disorders is constantly growing. Atomic force microscopy (AFM) is a well-established technic to study the mechanobiology of biological samples under physiological and pathological conditions at the cellular scale. However, a review which described all the different data that can be obtained using the AFM (cell elasticity, adhesion behavior, viscoelasticity, beating force, and frequency) is still missing. In this review, we will discuss several techniques that highlight the potential of AFM to be used as a tool for assessing the biomechanics involved in ACM. Indeed, analysis of genetically mutated cells with AFM reveal abnormalities of the cytoskeleton, cell membrane structures, and defects of contractility. The higher the Young’s modulus, the stiffer the cell, and it is well known that abnormal tissue stiffness is symptomatic of a range of diseases. The cell beating force and frequency provide information during the depolarization and repolarization phases, complementary to cell electrophysiology (calcium imaging, MEA, patch clamp). In addition, original data is also presented to emphasize the unique potential of AFM as a tool to assess fibrosis in cardiac tissue.

show abstract

“…Veronique Lachaize and coworkers detailed, for this Special Issue, the biophysical and biomechanical impact of the LMNA D192G missense mutation on neonatal rat ventricular fibroblasts (NRVF). They evidenced a decreased elasticity, a disturbed cytoskeleton organization and altered cell-to-cell adhesion properties in the cardiac fibroblasts with LMNA D192G mutation [ 9 ]. As similar observations have been made before in cardiomyocytes with a mutation of LMNA D192G [ 10 ], the recent findings of Lachaize et al clearly indicate the importance of LMNA mutations for ACM, as in addition to cardiomyocytes, cardiac fibroblasts are also highly biomechanically impacted by this mutation [ 9 ].…”

mentioning

confidence: 99%

Transcriptional Regulation of Cardiac Development and Disease

Wagner

2022

IJMS

View full text Add to dashboard Cite

The heart is the first organ to form and function during embryogenesis. Early heart development involves complex biological signals, interactions, specification of myocardial progenitor cells, and heart tube looping. It leads to the formation of the four-chambered heart in mammals which, at birth, upon separation from the placental circulation, takes over to ensure the continuation of vital processes throughout life. The contribution of epicardial, endocardial, sinus venosus, and hematopoietic-derived cells to heart formation in development and cardiac repair is still controversial. Many transcriptional regulators involved in cardiac development and disease have been identified; however, their interactions and possible implications in cardiovascular diseases are not fully elucidated. Cardiovascular diseases represent the leading cause of death worldwide. Understanding the various aspects of the transcriptional regulation and identification of the distinct cell types involved in cardiac development and disease will help to translate the findings from basic science to applied therapeutic approaches. This Special Issue of International Journal of Molecular Science brings together the most recent advances in understanding the various aspects of cardiac development and disease and provides new insights into the transcriptional regulation and the complex biological processes underlying heart differentiation and pathology. Furthermore, the latest therapeutic perspectives in cardiovascular diseases are discussed.

show abstract

Compromised Biomechanical Properties, Cell–Cell Adhesion and Nanotubes Communication in Cardiac Fibroblasts Carrying the Lamin A/C D192G Mutation

Cited by 6 publications

References 79 publications

Application of optical tweezers in cardiovascular research: More than just a measuring tool

Application of optical tweezers in cardiovascular research: More than just a measuring tool

Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy

Transcriptional Regulation of Cardiac Development and Disease

Contact Info

Product

Resources

About