Comprehensive Transcriptome Analysis of mRNA Expression Patterns of Early Embryo Development in Goat under Hypoxic and Normoxic Conditions

Abstract

It has been reported that hypoxic environments were more suitable for the in vitro development of mammalian embryos, but the underlying mechanisms were still unclear. In the present study, RNA-seq was performed to compare 8-cell-stage and blastocyst-stage goat embryos under hypoxic and normoxic conditions; zygotes were checked at 72 and 168 h to 8-cell stage (L8C) and blastocyst stage (LM) in hypoxic conditions and 8-cell stage (H8C) and blastocyst stage (HM) in normoxic conditions. In the H8C and L8C groups, 399 DEGs were identified, including 348 up- and 51 down-regulated DEGs. In the HM and LM groups, 1710 DEGs were identified, including 1516 up- and 194 down-regulated DEGs. The expression levels of zygotic genes, transcription factors, and maternal genes, such as WEE2, GDF9, HSP70.1, BTG4, and UBE2S showed significant changes. Functional enrichment analysis indicated that these DEGs were mainly related to biological processes and function regulation. In addition, combined with the pathway–gene interaction network and protein–protein interaction network, twenty-two of the hub genes were identified and they are mainly involved in energy metabolism, immune stress response, cell cycle, receptor binding, and signal transduction pathways. The present study provides comprehensive insights into the effects of oxidative stress on early embryo development in goats.

Melatonin Alleviates Oxidative Stress Induced by H2O2 in Porcine Trophectoderm Cells

“…The KEGG pathway enrichment analysis showed that the Wnt signaling pathway, “TGF-beta signaling pathway” and “mTOR signaling pathways” were significantly enriched for DEGs between the MH and H 2 O 2 groups. The Wnt signaling pathway has been reported to be redox-sensitive and regulated by oxidative stress [ 47 , 48 , 49 ]. In the present study, DEGs were significantly enriched in the Wnt signaling pathway, suggesting that MT may play a protective role in H 2 O 2 -induced oxidative stress in PTr2 cells by regulating the Wnt signaling pathway.…”

Bioactive Materials Derived from Menstrual Blood Stem Cells Enhance the Quality of In Vitro Bovine Embryos

“…As known, higher oocytes developmental competence ensures a higher number of high quality blastocysts 18 . It is also evident that the quality of embryos has been affected by the culture condition by which they were exposed 19 , 20 . In the current study, the addition of bioactive materials derived from MenSCs in the oocyte maturation medium significantly increased the rate of blastocysts on day 8.…”