Comprehensive Sequence and Whole-Life-Cycle Expression Profile Analysis of the Phosphate Transporter Gene Family in Rice

Abstract

Plant phosphate transporter (PT) genes comprise a large family with important roles in various physiological and biochemical processes. In this study, a database search yielded 26 potential PT family genes in rice (Oryza sativa). Analysis of these genes led to identification of eight conserved motifs and 5-12 trans-membrane segments, most of them conserved. A total of 237 putative cis elements were found in the 2-kb upstream region of these genes. Of these, a majority were Pi-response and other stress-related cis regulatory elements, such as PHO-like, TATA-box-like, PHR1, or Helix-loop-helix elements, and WRKY1 and ABRE elements, suggesting gene regulation by these signals. Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from 27 tissues covering the entire lifecycle from three rice genotypes: Minghui 63, Zhenshan 97, and Shanyou 63. Real-time PCR analysis confirmed that three rice PT genes are preferentially expressed in stamen at 1 d before flowering, two in panicle at the heading stage, and two in flag leaf at 14 d after the heading stage. Hormone-treatment experiments revealed differential up-regulation or down-regulation of 11 rice PT genes in seedlings exposed to five hormones, respectively. These results will be useful for elucidating the roles of these genes in the growth, development, and stress response of the rice plant.

Phosphate Concentration and Arbuscular Mycorrhizal Colonisation Influence the Growth, Yield and Expression of Twelve PHT1 Family Phosphate Transporters in Foxtail Millet (Setaria italica)

“…AtPHT1;7 , AtPHT 1;8 and AtPHT1;9 are also expressed at low levels in Pi starved roots [69], while both AtPHT1;1 and AtPHT1;4 have been shown to play a major role in Pi acquisition in Arabidopsis [70], [71]. OsPHT1;4 and OsPHT1;8 were expressed in roots of 3 rice cultivars [66] and OsPHT1;6 was shown, through RNA interference, to play an important role in Pi uptake while OsPHT1;2 was suggested to be involved in the root to shoot transport of Pi [21]. Barley HvPHT1;1 , which is expressed in roots, is moderately up regulated by P deficiency and encodes a high affinity P transporter [72].…”

“…They include both low and high affinity transporters and whilst most family members are expressed in roots, individual members have complex and overlapping expression patterns reflecting different physiological functions [19], [66]. For example OsPHT1;6 is a high affinity transporter with an apparent K m of 97 µM when expressed in yeast, and is expressed in root epidermal cells where it is likely responsible for Pi uptake from the soil.…”

Influence of Sulfur on Transcription of Genes Involved in Arsenic Accumulation in Rice Grains

“…3), specifically phosphate transporter (PT) (Liu et al 2011) and aquaporin. A total of ten phosphate transporters [OsPT1 (LOC_Os03g05620.1),…”

Efficient Mineral Nutrition: Genetic Improvement of Phosphate Uptake and Use Efficiency in Crops

“…A constitutively expressed low-affinity uptake system with a K m of 50-300 μM and a high-affinity uptake system, which is regulated by P i availability with K m of 3-7 μM have been proposed (Ullrich-Eberius et al 1981;Furihata et al 1992;Preuss et al 2010). Phosphate transporters are classified into distinct families: Pht1, Pht2, Pht3 and Pht4 (Bucher et al 2001;Liu et al 2011).…”

“…The Pht3 transporters belong to the mitochondrial transporter family (Rausch and Bucher 2002) and the Pht4 family has been suggested to play a role in P i translocation between the cytosol, chloroplast, plastids and the Golgi apparatus (Guo et al 2008;Liu et al 2011).…”