Comprehensive separation of major compositions from Sophora japonica var. violacea by counter-current chromatography using a liquid-liquid extraction strategy

Yang, Jiao; Gu, Dongyu; Ji, Zhenni; Chen, Fang; Xu, Fa; Yang, Yi

doi:10.1016/j.indcrop.2018.08.003

Cited by 27 publications

(10 citation statements)

References 25 publications

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“…13 C NMR (125 MHz, CD 3 OD) δ C : 116.0 (C-3/C-5), 122.9 (C-1), 132.9 (C-2/C-6), 163.2 (C-4), 170.4 (COOH). The 1 H and 13 C NMR spectroscopic data were in accordance with those of p-hydroxybenzoic acid [38].…”

Section: Structural Characterization Of Compoundssupporting

confidence: 73%

Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

et al. 2021

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Vaccinium dunalianum Wight, usually processed as a traditional folk tea beverage, is widely distributed in the southwest of China. The present study aimed to investigate the antioxidant, α-glucosidase and pancreatic lipase inhibitory activities of V.dunalianum extract and isolate the bioactive components. In this study, the crude extract (CE) from the buds of V. dunalianum was prepared by the ultrasound-assisted extraction method in 70% methanol and then purified with macroporous resin D101 to obtain the purified extract (PM). Five fractions (Fr. A–E) were further obtained by MPLC column (RP-C18). Bioactivity assays revealed that Fr. B with 40% methanol and Fr. D with 80% methanol had better antioxidant with 0.48 ± 0.03 and 0.62 ± 0.01 nM Trolox equivalent (TE)/mg extract for DPPH, 0.87 ± 0.02 and 1.58 ± 0.02 nM TE/mg extract for FRAP, 14.42 ± 0.41 and 19.25 ± 0.23 nM TE/mg extract for ABTS, and enzyme inhibitory effects with IC50 values of 95.21 ± 2.21 and 74.55 ± 3.85 for α-glucosidase, and 142.53 ± 11.45 and 128.76 ± 13.85 µg/mL for pancreatic lipase. Multivariate analysis indicated that the TPC and TFC were positively related to the antioxidant activities. Further phytochemical purification led to the isolation of ten compounds (1–10). 6-O-Caffeoylarbutin (7) showed significant inhibitory effects on α-glucosidase and pancreatic lipase enzymes with values of 38.38 ± 1.84 and 97.56 ± 7.53 µg/mL, and had the highest antioxidant capacity compared to the other compounds.

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Section: Structural Characterization Of Compoundssupporting

confidence: 73%

Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

et al. 2021

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“…Recently, several multicomponent liquid-liquid extraction-assisted sample pretreatment systems have been established, which can effectively remove impurities. Moreover, without changing the components, the two-phase solvent systems can be used for HSCCC separation with a slight modification of the polarity [31,32]. In the present study, firstly, the ethyl acetate extract was dissolved and extracted with the lower phase and upper phase of n-hexane-ethyl acetate-water (3:3:4, v/v/v), respectively, to remove the nontarget impurities in the mixture.…”

Section: Selection Of Extraction Solvent Systems For Sample Pretreatmentmentioning

confidence: 99%

“…The ethyl acetate extract was conducted by a series of two-phase solvent systems as descibed in the literature with minor modification [32]. Ethyl acetate extract (4 mg) was dissolved in the lower phase (1 mL) of n-hexane-ethyl acetate-water (5:1:4, v/v/v), and then extracted three times with the upper phase (1 mL).…”

Section: Selection Of Extraction Solvent Systems For Sample Pretreatmentmentioning

confidence: 99%

Identification of Transient Receptor Potential Vanilloid 3 Antagonists from Achillea alpina L. and Separation by Liquid-Liquid-Refining Extraction and High-Speed Counter-Current Chromatography

et al. 2020

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Bioassay-guided fractionation of the ethanol extract of whole herbs of Achillea alpina led to the isolation of isochlorogenic acids A and B as transient receptor potential vanilloid 3 (TRPV3) channel antagonists by using a calcium fluorescent assay. The structures were identified by spectroscopic analysis and the inhibitory activities of isochlorogenic acids A and B were confirmed by whole-cell patch clamp recordings of human embryonic kidney 293 (HEK293) cells expressing human TRPV3. Molecular docking results revealed that these two compounds reside in the same active pocket of human TRPV3 channel protein with lower binding energy than the agonist 2-aminoethoxydiphenyl borate (2-APB). High-speed counter-current chromatography (HSCCC) coupled with a liquid-liquid extraction approach was successfully established for the separation of isochlorogenic acids A and B from the whole herbs of A. alpina. Ethyl acetate and n-hexane-ethyl acetate-water (3:3:4 and 1:5:4, v/v/v) were selected as liquid-liquid extraction solvent systems to remove high- and low-polarity impurities in the mixture. Sixty g of ethanol extract was refined by solvent partition to yield 1.7 g of the enriched fraction, of which 480 mg in turn obtained 52.5 mg of isochlorogenic acid B (purity 98.3%) and 37.6 mg isochlorogenic acid A (purity 96.2%) after HSCCC with n-hexane-ethyl acetate-water containing 1% acetic acid (1:4:8, v/v/v).

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“…The water used was pretreated with a Super Pure Water system (TYP10S, Jinan Taipingma Environmental Protection Equipment Co., Ltd.). The plant material of S. officinalis used here was the same as that in our previous study, 14,15 as well as the medicine origin.…”

Section: Chemicals and Plant Materialsmentioning

confidence: 99%

An efficient method to obtain anti-inflammatory phenolic derivatives from Scindapsus officinalis (Roxb.) Schott. by a high speed counter-current chromatography coupled with a recycling mode

Sun

Zhao³

et al. 2020

RSC Adv.

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Comprehensive separation of major compositions from Sophora japonica var. violacea by counter-current chromatography using a liquid-liquid extraction strategy

Cited by 27 publications

References 25 publications

Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

Identification of Transient Receptor Potential Vanilloid 3 Antagonists from Achillea alpina L. and Separation by Liquid-Liquid-Refining Extraction and High-Speed Counter-Current Chromatography

An efficient method to obtain anti-inflammatory phenolic derivatives from Scindapsus officinalis (Roxb.) Schott. by a high speed counter-current chromatography coupled with a recycling mode

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