Comprehensive separation and identification of chemical constituents from Apocynum venetum leaves by high-performance counter-current chromatography and high performance liquid chromatography coupled with mass spectrometry

Extraction, Preconcentration and Isolation of Flavonoids from Apocynum venetum L. Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System

“…Aqueous biphasic systems based on ionic liquids (IL-ABS) were proposed in 2003 by Rogers [23], and have been developed to extract and isolate several bioactive compounds, such as anthraquinones [24], gallic acid [25], puerarin [26], and alkaloids [27]. ' The analysis and identification of the flavonoids in A. venetum leaves using liquid chromatography-tandem mass spectrometry (LC-MS/MS) are well reported in the literature [28][29][30]. The present article reports the extraction of flavonoids from A. venetum into IL aqueous solution by the IL-UAE method, with further application of IL-ABS for preconcentration and purification of the flavonoids.…”

“…effective method for the extraction of various compounds, such as aesculin and aesculetin from Cortex fraxini [17], piperine from white pepper [18], ginsenosides from ginseng root [19], alkaloids from Catharanthus roseus [20], biphenyl cyclooctene lignans from Schisandra chinensis Baill [21], magnolol and honokiol from Magnoliae officinalis cortex [22]. Aqueous biphasic systems based on ionic liquids (IL-ABS) were proposed in 2003 by Rogers [23], and have been developed to extract and isolate several bioactive compounds, such as anthraquinones [24], gallic acid [25], puerarin [26], and alkaloids [27].` The analysis and identification of the flavonoids in A. venetum leaves using liquid chromatography-tandem mass spectrometry (LC-MS/MS) are well reported in the literature [28][29][30]. The present article reports the extraction of flavonoids from A. venetum into IL aqueous solution by the IL-UAE method, with further application of IL-ABS for preconcentration and purification of the flavonoids.…”

Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

“…Current purification methods include the use of labor intensive and time-consuming columns with a variety of stationary phases and sequential solvent extraction [5][6][7][8]. High performance countercurrent chromatography (HPCCC) is a liquid-liquid chromatographic separation technique used for the isolation of natural compounds [9][10][11][12]. Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCCC has numerous advantages such as no irreversible adsorption and complete recovery in samples.…”

“…Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCCC has numerous advantages such as no irreversible adsorption and complete recovery in samples. Because of these advantages, HPCCC is gaining popularity as a purification tool for natural products [9][10][11]13].…”

Comparison of the constituents of Apocynum venetum and acidified Apocynum venetum by liquid chromatography–UV diode array detection–electrospray ionisation mass spectrometry

“…Apocynum venetum leaf extracts contain a lot of constituents with documented biological activity such as phenolic acids and a broad range of flavonoids (Zhang et al, 2010). Some researches have concluded that the total flavonoids of AV have antidepressant effect and the effect of those of AV acidified by H 2 SO 4 is stronger than that of those of AV, the researchers consider that the major active constituents are hyperoside (quercetin-3-O-galactoside) or isoquercetin (quercetin-3-O-glucoside), but the antidepressant effects of pure hyperoside or isoquercetin are not consistent with that of the crude extract of AV or AAV (Butterweck et al, 2001), so we concluded that the pharmacological activities of AV and AAV preparations may not depend on a single compound, but on the combined activities of the several constituents of AV.…”