Comprehensive review of trends and analytical strategies applied for biological samples preparation and storage in modern medical lipidomics: State of the art

Jurowski, Kamil; Kochan, Kamila; Walczak, Justyna; Barańska, Małgorzata; Piekoszewski, Wojciech; Buszewski, Bogusław

doi:10.1016/j.trac.2016.10.014

Cited by 40 publications

(25 citation statements)

References 136 publications

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“…Since most of the extraction protocols described below are based on the SPE and LLE methods, the question arises—which of these protocols is more suitable for lipidomics studies. The advantages and disadvantages of PPT, LLE and SPE have been reviewed comprehensively elsewhere [ 91 , 92 , 93 , 94 , 95 , 96 ] and discussions on which of the methods is better are still ongoing. Among the main advantages of the LLE, one can note its low costs and the presence of a large number of well-established protocols.…”

Section: Methods Of Sample Preparation For Lipidomic Studiesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also used in healthy, nonobese subjects or patients with other dyslipidemias. This review is an overview of sample preparation methods for analysis as one of the major critical steps in the overall analytical procedure.

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Section: Methods Of Sample Preparation For Lipidomic Studiesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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“…Liquid–liquid extraction (LLE) and solid phase extraction (SPE) are two methods commonly used for both biological samples and food samples (Jurowski et al., 2017; Pati, Nie, Arnold, & Cummings, 2016). The classical LLE methods developed by Folch et al.…”

Section: The Analysis Workflow Of Ms‐based Lipidomicsmentioning

confidence: 99%

Mass spectrometry‐based lipidomics in food science and nutritional health: A comprehensive review

Sun

Wang

Cong

et al. 2020

Comp Rev Food Sci Food Safe

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With the advance in science and technology as well as the improvement of living standards, the function of food is no longer just to meet the needs of survival. Food science and its associated nutritional health issues have been increasingly debated. Lipids, as complex metabolites, play a key role both in food and human health. Taking advantages of mass spectrometry (MS) by combining its high sensitivity and accuracy with extensive selective determination of all lipid classes, MS‐based lipidomics has been employed to resolve the conundrum of addressing both qualitative and quantitative aspects of high‐abundance and low‐abundance lipids in complex food matrices. In this review, we systematically summarize current applications of MS‐based lipidomics in food field. First, common MS‐based lipidomics procedures are described. Second, the applications of MS‐based lipidomics in food science, including lipid composition characterization, adulteration, traceability, and other issues, are discussed. Third, the application of MS‐based lipidomics for nutritional health covering the influence of food on health and disease is introduced. Finally, future research trends and challenges are proposed. MS‐based lipidomics plays an important role in the field of food science, promoting continuous development of food science and integration of food knowledge with other disciplines. New methods of MS‐based lipidomics have been developed to improve accuracy and sensitivity of lipid analysis in food samples. These developments offer the possibility to fully characterize lipids in food samples, identify novel functional lipids, and better understand the role of food in promoting healt.

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“…A comprehensive list of vast lipids classes and subclasses combined with their common abbreviations can be found in the classification system provided by Lipid MAPS consortium [8]. Lipidomics studies can provide critical insights toward the development of an improved understanding of lipids metabolism and for the identification of new biomarkers or therapeutic targets of related diseases mentioned above [3,[9][10][11][12][13]. In the past 5 years, a variety of reviews have been published on the applications of lipidomics in different aspects, such as infection and inflammation [14], biology and medicine [10,15,16], clinical lipidomics [17,18], and lipid peroxidation-related diseases [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Recent progresses of derivatization approaches in the targeted lipidomics analysis by mass spectrometry

Zhao

Zhu

Liu

2020

J of Separation Science

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Lipidomics plays an essential role in the development of an improved understanding of lipids metabolism and the identification of new biomarkers or therapeutic targets of related diseases. The strong analytical power of mass spectrometry and its rapid developments in the respect of instruments and techniques have significantly accelerated the emerging lipidomics and related application fields in biology, medicine, and pharmacy. The strategy of chemical derivatization can remarkably improve the shortcomings of mass spectrometric analytical technologies of shotgun lipidomics and liquid chromatography mass spectrometry, and in the past decade many related studies have been reported for fatty acids, glycerophospholipids, sphingomyelins, monoglycerides, diacylglycerols, long-chain bases, steroids, and so on. Therefore, this review will focus on new chemical derivatization approaches about the research progresses of shotgunbased and liquid chromatography mass spectrometry-based targeted lipidomics (from 2005 to July 2019, most of reports emerged in the past 5 years), and put forward the problems and prospects in this field. It is expected to be helpful for the design and synthesis of new derivatization reagents, especially the outstanding stable isotope labeling derivatization reagents, and the development and application of new chemical derivatization strategies and matched mass spectrometric analysis methods. K E Y W O R D Schemical derivatization, lipidomics, liquid chromatography mass spectrometry, shotgun lipidomics, stable isotope labeling 1838

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Comprehensive review of trends and analytical strategies applied for biological samples preparation and storage in modern medical lipidomics: State of the art

Cited by 40 publications

References 136 publications

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

Mass spectrometry‐based lipidomics in food science and nutritional health: A comprehensive review

Recent progresses of derivatization approaches in the targeted lipidomics analysis by mass spectrometry

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