Comprehensive Proteomic Characterization of the Pectoralis Major at Three Chronological Ages in Beijing-You Chicken

Abstract

Chronological age is one of the important factors influencing muscle development and meat quality in chickens. To evaluate the protein expression profiles during skeletal muscle development, we performed a tandem mass tag (TMT)-based quantitative proteomic strategy in pectoralis major (breast muscle) of Beijing-You chicken (BYC) at the chronological age of 90, 120, and 150 days. Each chronological age contained 3 pooling samples or 15 birds (five birds per pooling sample). A total of 1,413 proteins were identified in chicken breast muscle with FDR < 1% and 197 of them were differentially expressed (fold change ≥1.2 or ≤0.83 and p < 0.05). There were 110 up- and 71 down-regulated proteins in 120 d vs 90 d group, 13 up- and 10 down-regulated proteins in 150 d vs 120 d group. The proteomic profiles of BYC at 120 d were very similar to those at 150 d and highly different from those at 90 d, suggesting that 120 d might be an important chronological age for BYC. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that these differentially expressed proteins were mainly involved in the pathway of glycolysis/gluconeogenesis, adrenergic signaling in cardiomyocytes, focal adhesion, oocyte meiosis and phagosome. Furthermore, some DEPs were quantified using parallel reaction monitoring (PRM) to validate the results from TMT analysis. In summary, these results provided some candidate protein-coding genes for further functional validation and contribute to a comprehensive understanding of muscle development and age-dependent meat quality regulation by proteins in chickens.

Hepatic Proteomics Analysis Reveals Attenuated Endoplasmic Reticulum Stress in Lactiplantibacillus plantarum-Treated Oxidatively Stressed Broilers

“…Thereafter, the peptide was labeled with TMT (Thermo Fisher Scientific, Waltham, MA), and the TMT-labeled peptides were fractionated on an Agilent 1290 HPLC operating at 0.3 mL/min using a Waters XBridge BEH130 column (C18, 3.5 μm, 2.1 mm × 150 mm). The fractions were dried for nanoliquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis according to a previous study …”

Breast Meat Fatty Acid Profiling and Proteomic Analysis of Beijing-You Chicken During the Laying Period

“…To validate the TMT proteomics data, further liquid chromatography-parallel reaction monitoring MS (LC-PRM/MS) analysis was performed ( 30 ). PRM analysis was performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) and the optimized methods were generated experimentally using 1–3 unique peptides of high intensity and confidence for each target protein.…”

“…However, these studies have mainly focused on IMF ( 26 , 27 ) and abdominal fat ( 31 , 32 ). Most of them were limited on embryonic muscle ( 28 , 29 ) or during the bird body development stage ( 30 , 31 ). To date, there was no report on the dynamic changes in FA composition and related proteins in chicken during the laying period, especially from the sexual maturation stage to the culling stage.…”

Genome-wide investigation to assess copy number variants in the Italian local chicken population

“…Some of the annotated genes within the CNVR have been already associated to functional traits in chickens (Additional le 5). The CNVR located on chromosome 1, shared among different animals, were used to annotate several genes, the most noteworthy being CACNA2D1 (calcium voltage-gated channel auxiliary subunit alpha 2 delta 1) which is related to muscle contraction [41]; DACH1 (dachshund family transcription factor 1), involved in skeletal development and inhibitor of growth factor beta [42,43]; DMD (dysthrophin), one of the most important factors for muscle development and structural stability of the tissue [44]; and NTS (neurotensin) which is associated to neuronal network work ow [45]. Noteworthy, the gene encoding the miRNAs, MIR7445-1, is present within a mixed CNVR identi ed in several animals belonging to different breeds (Additional le 5).…”