Comprehensive Proteomic Analysis of Exosomes Derived from Human Bone Marrow-, Adipose Tissue-, and Umbilical Cord- Mesenchymal Stem Cells

Wang, Zheng-Gang; He, Zhiyi; Liang, Shuang; Yang, Qing; Peng, Cheng; Chen, Anmin

doi:10.21203/rs.3.rs-83181/v1

Cited by 12 publications

(15 citation statements)

References 40 publications

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“…Recent studies have started to reveal the tissue-specific characteristics for MSC-EVs [15,16] . Our current study sought to investigate the MSC-EVs of dental pulp and adipose tissue, and demonstrated that they possess distinct transcriptomic characteristics indicative of their potential therapeutic targets.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles from mesenchymal stem cells of dental pulp and adipose tissue display distinct transcriptomic characteristics suggestive of potential therapeutic targets

Terunuma

Yoshioka

Sekine

et al. 2021

JSRM

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Objective: Mesenchymal stem cells (MSCs) are isolated from various human tissues and used for therapy, in which beneficial effects are attributed mainly to mesenchymal stem cell-derived extracellular vesicles (MSC-EVs). Whereas MSCs of diverse tissue types share cardinal stem cell features, it is becoming evident that MSCs of each tissue type possess unique properties as well. For designing efficient stem cellbased therapies, it is crucial to understand the unique properties associated with MSCs and MSC-EVs of each tissue type. Such unique properties can be analyzed through transcriptomic approaches using comprehensive gene expression databases and sophisticated analytical tools. Here, we comparatively studied the transcriptomes in MSC-EVs of dental pulp and adipose tissue. Additionally, the transcriptomes of MSC-EVs were compared with the cellular transcriptomes of MSCs for the same tissue types.Methods: MSCs were cultured from human dental pulp and adipose tissue specimens. Conditioned culture media were collected to prepare MSC-EVs, from which RNAs were isolated and subjected to next-generation sequencing for transcriptomic analysis. Gene expression signatures in MSC-EVs of each tissue type were investigated using gene set analysis.Results: MSC-EVs obtained from dental pulp-derived MSCs showed distinct transcriptomic signatures of neurogenesis and neural retina development while MSC-EVs of adipose tissue-derived MSCs showed signatures of mitochondrial activity and skeletal system development. The transcriptomes of MSC-EVs resembled the cellular transcriptomes of MSCs, and the genes associated with neurogenesis were highly expressed in both MSCs and MSC-EVs of dental pulp. Adipose tissue-derived MSCs and MSC-EVs highly expressed genes associated with angiogenesis, hair growth, and dermal matrices. Conclusion:The clear and distinct signatures of neurogenesis and neural retina development in dental pulp-derived MSC-EVs imply neurodegenerative disorders and retinal diseases as putative therapeutic targets. In contrast, the transcripts in adipose tissue-derived MSC-EVs could be useful in rejuvenating the skin and musculoskeletal system. Further insights into MSC-EVs of divergent tissue types may expand the list of potential therapeutic targets.Previously, we chose to analyze mRNAs in MSCs by taking advantage of comprehensive transcriptome databases and powerful analytical tools. Transcriptomes in MSCs of dental pulp and adipose tissue displayed unique transcriptomic characteristics for the MSCs of each tissue type [10] . In the current study, we investigate whether MSC-EVs of dental pulp and adipose tissue show distinct transcriptomes, and whether MSC-EVs and MSCs of the same tissue type share the transcriptomic characteristics. Materials and methods Cell sources and extracellular vesicle preparationHuman adipose tissues and exfoliated deciduous teeth were obtained from healthy donors with written informed consent. MSCs were cultured from the tissues to collect conditioned media at passage 7. Ninety-six ml of the M...

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Section: Discussionmentioning

confidence: 99%

Extracellular vesicles from mesenchymal stem cells of dental pulp and adipose tissue display distinct transcriptomic characteristics suggestive of potential therapeutic targets

Terunuma

Yoshioka

Sekine

et al. 2021

JSRM

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“…Interestingly, along with the structural roles of these lipids, they have also been implicated in signal transduction, cell cycle arrest, tumor suppression, and apoptosis [44]. [46]. These data may reflect the impact of exosome source on the exosome content and perhaps function.…”

Section: Molecular Composition Of Msc-evsmentioning

confidence: 99%

Molecular Insights into the Potential of Extracellular Vesicles Released from Mesenchymal Stem Cells and Other Cells in the Therapy of Hematologic Malignancies

Alsagaby

2021

Stem Cells International

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Hematologic cancer encompasses the heterogeneous group of neoplasms that affect different stages of blood cell linages. Despite the significant improvements made in the new modalities of anticancer therapy, many forms of blood cancer remain untreatable, putting the afflicted patients at high risk of death. Therefore, there has been an urgent need for novel therapy to improve the clinical outcomes of patients with blood cancer. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been reported to possess an anticancer activity. This review discusses (i) the therapeutic potential of MSC-EVs against blood cancer, (ii) the possibility of using EVs from sources other than MSCs as a mean for blood cancer vaccination and drug delivery, and (iii) areas to be optimized for MSC-EV-based clinical application on blood malignancies.

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“…Even though that study was conducted ex vivo , it reveals that EVs derived from different cell types have variable effects. Moreover, a proteomic analysis of exosomes isolated from BMSCs, ADSCs and umbilical cord MSCs demonstrated their potential utility in a variety of fields [ 84 ].…”

Section: Sources Of Evsmentioning

confidence: 99%

Extracellular Vesicles: a Promising cell-free Therapy for Cartilage Repair

et al. 2021

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Few effective therapies for cartilage repair have been found as cartilage has a low regenerative capacity. Extracellular vesicles (EVs), including exosomes, are produced by cells and contain bioactive components such as nucleic acids, proteins, lipids and other metabolites that have potential for treating cartilage injuries. Challenges like the difficulty in standardizing targeted therapy have prevented EVs from being used frequently as a treatment option. In this review we present current studies, mechanisms and delivery strategies of EVs. Additionally, we describe the challenges and future directions of EVs as therapeutic agents for cartilage repair.

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Comprehensive Proteomic Analysis of Exosomes Derived from Human Bone Marrow-, Adipose Tissue-, and Umbilical Cord- Mesenchymal Stem Cells

Cited by 12 publications

References 40 publications

Extracellular vesicles from mesenchymal stem cells of dental pulp and adipose tissue display distinct transcriptomic characteristics suggestive of potential therapeutic targets

Extracellular vesicles from mesenchymal stem cells of dental pulp and adipose tissue display distinct transcriptomic characteristics suggestive of potential therapeutic targets

Molecular Insights into the Potential of Extracellular Vesicles Released from Mesenchymal Stem Cells and Other Cells in the Therapy of Hematologic Malignancies

Extracellular Vesicles: a Promising cell-free Therapy for Cartilage Repair

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