Comprehensive long non-coding RNA expression profiling reveals their potential roles in systemic lupus erythematosus

Li, Lian-Ju; Zhao, Wei; Tao, Sha‐Sha; Li, Jun; Xu, Shu-Zhen; Wang, Jie‐Bing; Leng, Rui‐Xue; Fan, Yin‐Guang; Pan, Hai‐Feng; Ye, Dong‐Qing

doi:10.1016/j.cellimm.2017.06.004

Cited by 44 publications

(45 citation statements)

References 61 publications

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“…As previously described, 24 patients with SLE and 12 age‐matched healthy volunteers with no history of autoimmune diseases were recruited from Anhui Provincial Hospital and the First Affiliated Hospital of Anhui Medical University. The patients with SLE who were included fulfilled at least four of the American College of Rheumatology 1997 revised criteria of SLE .…”

Section: Methodsmentioning

confidence: 99%

“…RNA samples from T cells of six SLE patients and three healthy controls were used for circRNA microarrays. The cell isolation and RNA extraction were performed as previously described . Of note, the RNA samples were previously extracted for measuring lncRNA and mRNA expression profiles .…”

Section: Methodsmentioning

confidence: 99%

“…The cell isolation and RNA extraction were performed as previously described . Of note, the RNA samples were previously extracted for measuring lncRNA and mRNA expression profiles . We simultaneously obtained the expression of circRNAs and mRNAs in T cells, which was important for revealing the underlying mechanisms of functional circRNAs.…”

Section: Methodsmentioning

confidence: 99%

“…Extracted total RNA was reverse‐transcribed to cDNA with random primer using a RevertAid™ First‐Strand cDNA Synthesis Kit (Fermentas, Waltham, MA). The real‐time quantitative PCR was performed as previously described . The primer sequences are listed in Table .…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, studies from home and abroad have shown that a subset of non‐coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non‐coding RNAs (lncRNAs), also play a key role in SLE . Our recent study with human lncRNA microarrays demonstrated abnormal expression of thousands of mRNAs and lncRNAs in T cells from patients with SLE . However, research on the relevance of recently discovered circular RNAs (circRNAs) in this elusive condition is relatively scarce.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA expression profile and potential function of hsa_circ_0045272 in systemic lupus erythematosus

Zhu

Zhao

et al. 2018

Immunology

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Circular RNAs (circRNAs) represent a class of non-coding RNAs that form covalently closed RNA circles with extensive expression and conservation in mammals. Circular RNAs regulate gene expression through acting as competitive endogenous RNAs (ceRNAs) and modulating gene transcription. Accumulating evidence supports the implication of circRNAs in a variety of human diseases, but studies of circRNA role in systemic lupus erythematosus (SLE) are lacking. The present study measured the circRNA expression profiles in T cells from patients with SLE and healthy controls with human circRNA microarray and identified 127 differentially expressed circRNAs in SLE patients. Down-regulation of hsa_circ_0045272 in SLE T cells was verified with quantitative PCR. Jurkat cells with stable hsa_circ_0045272 knockdown were generated using specific lentiviral short hairpin RNA for functional studies. Flow cytometric analysis indicated that hsa_circ_0045272 knockdown significantly up-regulated the early apoptosis of Jurkat cells. Meanwhile, ELISA showed that hsa_circ_0045272 knockdown significantly enhanced interleukin-2 production of activated Jurkat cells. Then, ceRNAs were predicted for hsa_circ_0045272 and the significant down-regulation of two mRNAs predicted as ceRNAs, NM_003466 (PAX8) and NM_015177 (DTX4), but not their corresponding proteins, was validated. Furthermore, dual luciferase reporter assay indicated binding of hsa_circ_0045272 with hsa-miR-6127. Circular RNA-mRNA co-expression networks showed the correlation of circRNAs with mRNAs and provided additional clues to circRNA functions. Our study demonstrated dysregulated circRNAs in SLE and revealed the function of hsa_circ_0045272 in negatively regulating apoptosis and interleukin-2 secretion and its potential mechanism. The implication of hsa_circ_0045272 and other abnormal circRNAs in SLE merits further investigation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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