Long Non-coding RNAs in Rheumatology

Wijesinghe, Susanne N.; Lindsay, Mark A.; Jones, Simon W.

doi:10.1007/978-3-030-92034-0_4

Cited by 2 publications

(2 citation statements)

References 201 publications

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“…Accumulating studies have shown that dysregulated lncRNAs contribute to the pathogenesis of OA by influencing CH proliferation, inflammatory responses, and angiogenesis. 38 , 39 COX‐2, an enzyme that catalyzes arachidonic acid to produce prostaglandins, can be induced by cytokines and inflammatory signals to participate in various physiological and pathological processes such as inflammation and pain. 40 LncRNA PACER has been demonstrated to promote the expression of COX‐2 by binding to the inhibitory transcription factor p50.…”

Section: Discussionmentioning

confidence: 99%

FSGT capsule inhibits IL‐1β‐induced inflammation in chondrocytes and ameliorates osteoarthritis by upregulating LncRNA PACER and downregulating COX2/PGE2

He,

Liu,

Sun

et al. 2024

Immunity Inflam & Disease

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ObjectiveTo explore the efficacy and potential mechanism of Fengshi Gutong capsule (FSGTC) in osteoarthritis (OA) inflammation.MethodsThe impact of FSGTC on laboratory indicators of OA patients was explored using data mining technology and association rule analysis. Then, the OA cell model was constructed by inducing chondrocytes (CHs) with interleukin‐1β (IL‐1β). In the presence of FSGTC intervention, the regulatory mechanism of PACER/COX2/PGE2 in OA‐CH viability and inflammatory responses was evaluated.ResultsRetrospective data mining showed that FSGTC effectively reduced inflammation indexes (ESR, HCRP) of OA patients. Cell experiments showed that LncRNA PACER (PACER) silencing inhibited the proliferation activity of OA‐CHs, increased the level of COX2 protein, elevated the levels of PGE2, TNF‐α, and IL‐1β, and decreased the levels of IL‐4 and IL‐10 (p < .01). On the contrary, FSGTC‐containing serum reversed the effect of PACER silencing on OA‐CHs (p < .01). After the addition of COX2 pathway inhibitor, the proliferation activity of OA‐CHs was enhanced; the levels of PGE2, TNF‐α, and IL‐1β were decreased while the levels of IL‐4 and IL‐10 were increased (p < .01).ConclusionFSGTC inhibits IL‐1β‐induced inflammation in CHs and ameliorates OA by upregulating PACER and downregulating COX2/PGE2.

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Section: Discussionmentioning

confidence: 99%

FSGT capsule inhibits IL‐1β‐induced inflammation in chondrocytes and ameliorates osteoarthritis by upregulating LncRNA PACER and downregulating COX2/PGE2

He,

Liu,

Sun

et al. 2024

Immunity Inflam & Disease

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“…In spite of identifying these lncRNAs, very few of them have been functionally investigated in SLE till now. These lncRNAs include GAS5, MALAT1, TUG1, NEAT1, UCA1, XIST and THRIL [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

The potential role of serum expression profile of long non coding RNAs, Cox2 and HOTAIR as novel diagnostic biomarkers in systemic lupus erythematosus

Mahmoud

Fouad²,

Hefzy³

et al. 2022

PLoS ONE

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Background The role of the long non-coding RNAs (lncRNAs) in the pathogenesis of systemic lupus erythematosus (SLE) is mostly unknown, despite increasing evidence that lncRNAs extensively participate in physiological and pathological conditions. Aim To detect the level of lncRNA-Cox2, HOTAIR, IL-6, and MMP-9 in the serum of SLE patients and to correlate these levels with disease activity and patients’ clinical and laboratory data to evaluate the value of these biomarkers for SLE diagnosis and assessment of disease activity. Methods Blood samples from 58 SLE patients, and 60 healthy controls (HCs) were used for detection of lncRNAs-Cox2 and HOTAIR expression levels by real-time polymerase chain reaction. Both IL-6 and MMP-9 serum levels were assayed by enzyme-linked immunosorbent assay. Lupus activity was assessed with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Results The serum expression levels of lncRNA-Cox2 and HOTAIR were significantly up-regulated in SLE patients vs HCs (fold change [median (IQR) was 1.29(0.81–1.71, P<0.0001) and 2.68(0.95–3.67), P = 0.038) for lncRNA-Cox2 and HOTAIR, respectively. Serum levels of both IL-6 and MMP-9 were significantly high in SLE patients compared with HCs (P≤0.001 for each). The up-regulated lncRNA-Cox2 was positively associated with the presence of neurological manifestations in SLE patients (P = 0.007). Furthermore, HOTAIR expression level had significantly positive correlation with IL-6 (r = 0.578, P<0.0001), MMP-9 level (r = 0.762, P<0.0001), nephritis grades (r = 0.296, P = 0.024) and proteinuria (r = 0.287, P = 0.035). LncRNA-Cox2 showed sensitivity and specificity 72.4%, and 100.0% respectively. HOTAIR sensitivity was 60.3%, and specificity was 100.0%. By multiple logistic regression analysis, lncRNA-Cox2 and HOTAIR were found as SLE independent predictors. Conclusion LncRNA-COX2 and HOTAIR can be used as new non-invasive biomarkers for the diagnosis of SLE.

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Long Non-coding RNAs in Rheumatology

Cited by 2 publications

References 201 publications

FSGT capsule inhibits IL‐1β‐induced inflammation in chondrocytes and ameliorates osteoarthritis by upregulating LncRNA PACER and downregulating COX2/PGE2

FSGT capsule inhibits IL‐1β‐induced inflammation in chondrocytes and ameliorates osteoarthritis by upregulating LncRNA PACER and downregulating COX2/PGE2

The potential role of serum expression profile of long non coding RNAs, Cox2 and HOTAIR as novel diagnostic biomarkers in systemic lupus erythematosus

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