Comprehensive Immunological Study of Persons Vaccinated with Live Plague Vaccine Living on the Territory of the Pre-Caspian Sand Foci of the Plague in the Republic of Kalmykia

Бугоркова, С. А.; Щуковская, Т. Н.; Микшис, Н. И.; Клюева, С. Н.; Кудрявцева, О. М.; Kravtsov, A. L.; Goncharova, A. Yu.; Кожевников, В. А.; Санджиев, Д. Н.; Конушева, С. В.; Савченко, С. П.; Бембеева, Е. С.; Щербакова, Светлана; Кутырев, Владимир

doi:10.31631/2073-3046-2018-17-3-38-50

Cited by 9 publications

(5 citation statements)

References 9 publications

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“…Иммунофенотипирование лимфоцитов в об разцах цельной крови проводили по Lyse/ NoWash протоколу проточноцитофлуориметри ческих исследований, разработанному фирмой BD Biosciences (USA) [11], чтобы максимально стандартизировать процедуру пробоподготовки для цитометрического анализа, повысить её про изводительность и полностью исключить кле точные потери [12]. Для окраски клеток крови применяли четырёхцветный реагент меченых моноклональных антител CytoStat CD45FITC, CD4PE, CD8ECD, CD3PC5 (Backman Coulter, US), с помощью которого в крови определяли относи тельное содержание Тхелперов (СD45 + CD3 + CD4 + ) и цитотоксических Тклеток (СD45 + CD3 + CD8 + ) для вычисления индивидуальных значений иммуноре гуляторных индексов (ИРИ) [4]. В тесте in vitro cо специфическим стимулятором интенсивность ак тивации Тхелперов оценивали через 24 ч инку бации [9].…”

Section: материалы и методыunclassified

“…Задача оценки иммунологической эффектив ности вакцины живой чумной (ВЧЖ), используемой для специфической профилактики чумы, до конца не решена, так как клеточные механизмы антибактери альной защиты при этой инфекции недостаточно из учены [1,2] и нормативная база, регламентирующая определение уровня клеточного противочумного иммунитета у людей, на сегодняшний день отсутству ет [2][3][4][5]. Успешность решения данной задачи во многом зависит от автоматизации и стандартизации цитологических исследований [6,7], направленных на выявление ключевых параметров реактивности клеток иммунной системы привитых против чумы лиц в условиях in vitro [5,8].…”

Section: Introductionunclassified

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Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Кожевников

Kravtsov

Кудрявцева

et al. 2022

Epidemiology and Vaccinal Prevention

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Relevance. The search for informative markers for assessing the immunological efficacy of a live plague vaccine remains an urgent scientific task. The goal of the studyAims was to make a comparative assessment of the level helper T-cells activation by CD69 and HLA-DR markers, in people, vaccinated against plague, in an in vitro test, using the disintegrated by ultrasonic Y. pestis cells, grown at temperature 28 °C as a specific stimulator.Materials & Methods. A cytofluorimetric analysis of blood samples of 45 individuals vaccinated against the plague was carried out. The dependence of the result of cytological analysis of the two studied cell markers on the immunoregulatory index (IRI) of the vaccinated donor at the time of vaccination was revealed.Results. It was found that for newly vaccinated individuals, the T-helpers were more intensive and prolonged by the early activation marker, while for those revaccinated with the immunoregulatory index, at the time of vaccination more than 1.5, the more intense cellular response was by the late activation marker.Conclusions. Thus, the possibility of quantitative evaluation of the immunological efficacy of vaccination against plague, based on the identification of lymphocyte activation markers when stimulated with a specific antigen, is confirmed.

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Section: материалы и методыunclassified

Section: Introductionunclassified

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Кожевников

Kravtsov

Кудрявцева

et al. 2022

Epidemiology and Vaccinal Prevention

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“…Результаты. Иммунологическое обследование привитых против чумы добровольцев, проживающих на территории Прикаспийского песчаного природного очага чумы, проводили в течение трех лет по разработанному ранее алгоритму [9], оценивая динамику изменения показателей клеточного и гуморального иммунного ответа на ВЧЖ.…”

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The Analysis of Factors Influencing Immunologic Reactivity in People Vaccinated with a Live Plague Vaccine

Кудрявцева¹,

Goncharova²,

Бугоркова³

et al. 2020

PH&LE

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Introduction: An increase in epizootic activity has been registered in a number of plague foci in the Russian Federation over the past few years. As part of securing sanitary and epidemiologic wellbeing of the population living in the natural foci of the disease, a mass immunization with a live plague vaccine based of the Yersinia pestis EV line NIIEG vaccine strain was carried out. Objectives: The purpose of the study was to assess the influence of a complex of factors including age, gender, health status, the number of previous vaccinations against plague, blood groups, and HLA gene polymorphism on the state of the cellular and humoral immune response in people vaccinated with the live plague vaccine. Materials and methods: The analysis of venous blood of 347 volunteers included determination of the concentration of specific antibodies to the capsular antigen (F1) of plague microbe, spontaneous and induced production of marker cytokines (IFN-γ, TNF-α, and IL-4) by ELISA, and genes of the main histocompatibility complex (HLA) class II by real-time PCR. We also analyzed medical documentation (Form 025/u) and the results of a questionnaire-based survey of the vaccinated people. Results and discussion: We established the influence of various factors, including genetic ones, on marker indicators of the humoral and cellular immune response in persons vaccinated with the live plague vaccine. We also characterized the relationship between the level of specific antibodies to plague microbe F1 production and some cytokines and the age and the number of previous vaccinations in our volunteers. The most common gene variants of the main histocompatibility complex of class II (HLA-DQA1, HLA-DQB1 and HLA-DRB1) in the cohort were identified and possible relationships between the production of IFN-γ, TNF-α, IL-4 and allelic polymorphism of HLA class II genes were determined. Conclusions: Immunologic reactivity in people vaccinated with the live plague vaccine is mainly determined by age, the number of previous vaccinations against this infection, and individual characteristics of HLA gene polymorphism.

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“…Immunologic monitoring also involves characterizing immunologic restricting in vaccinated volunteers during 12-month monitoring period; we determined ranges of fluctuations in individual immune responses to LPV depending on sex, age, number of previous vaccinations and HLA haplotype of vaccinated people [13].…”

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confidence: 99%

“…We characterized induced production of Th1 (INF-ɣ, TNF-α) and Th2 (IL-4) associated cytokines in dynamics and established that there was significant Th1-cells activation aimed at producing cytokines associated with them during the whole monitoring period [13]. As an inductor, Concavalin A, a standard commercial T-cell mitogen and TLR2 ligand, was applied [14].…”

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confidence: 99%

Immunologic monitoring over people vaccinated against plague in caspian sand natural focus in order to assess and manage health risks

Бугоркова¹,

Клюева²,

Кудрявцева³

et al. 2020

Health Risk Analysis

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To provide better opportunities for managing both risks caused by vaccination and risks of epidemiological complications, immunologic monitoring over people vaccinated with live dried plague vaccine (LPV) due to epidemiologic indications was performed. Our research goal was to assess whether immunologic monitoring over people vaccinated against plague yielded informative results; it was done to substantiate activities aimed at improving procedures for LPV application. Immunologic monitoring was performed from 2016 to 2019 in the Caspian sand natural plague focus according to conventional procedures for assessing humoral and cellular components in immunity. We determined immunologic parameters in 217 volunteers vaccinated with LPV and 130 healthy donors (the reference group) prior to and 1 and 12 months after vaccination. We suggested a methodical approach based on aggregated analysis of the summated immune response predictors chosen for estimation in volunteers vaccinated with LPV and giving score values to them; it allows revealing people who react to plague microbe antigens predominantly as per cellular, humoral, or mixed type. Immunologic monitoring results proved that it was safe to apply LPV; they allowed characterizing trends occurring in immunological restructuring in vaccinated volunteers, determining limits of fluctuation in individual parameters of an immune response to the vaccine, and revealing people with both normal and changed (reduced or increased) immunologic reactivity to LPV. If monitoring data are taken into account, it provides an opportunity to predict vaccination results as per epidemiological parameters, to reveal groups with normal, high, or low immune reactivity to plague microbe antigens in order to determine people in them who need an individual approach when it comes down to anti-plague vaccination.

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Comprehensive Immunological Study of Persons Vaccinated with Live Plague Vaccine Living on the Territory of the Pre-Caspian Sand Foci of the Plague in the Republic of Kalmykia

Abstract: Comprehensive Immunological Study of Persons Vaccinated with Live Plague Vaccine Living on the Territory of the Pre-Caspian Sand Foci of the Plague in the Republic of Kalmykia

Cited by 9 publications

References 9 publications

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

The Analysis of Factors Influencing Immunologic Reactivity in People Vaccinated with a Live Plague Vaccine

Immunologic monitoring over people vaccinated against plague in caspian sand natural focus in order to assess and manage health risks

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