Comprehensive dissection of primary metabolites in response to diverse abiotic stress in barley at seedling stage

Zhao, Hongwei; Ni, Shengjing; Cai, Shengguan; Zhang, Guoping

doi:10.1016/j.plaphy.2021.01.048

Cited by 22 publications

(28 citation statements)

References 43 publications

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“…Our understanding of plant response to environmental stresses has been significantly improved due to the rapid development of multi-omics technologies, such as genome sequencing, [18] whole genome bisulfite sequencing (WGBS, DNA methylome), [19] transcriptome, [20] proteome, [21] and metabolome. [22,23] Many of these technologies have been extensively employed for prescreening of elite germplasm and identification of novel alleles, epialleles, proteins, and metabolites to facilitate the process of molecular breeding for climate resilient crops. [24,25] Strong regulation of DNA methylation on transcriptome was extensively reported when plants are exposed to environmental stresses.…”

Section: Introductionmentioning

confidence: 99%

Multi‐Omics Analysis Reveals the Mechanism Underlying the Edaphic Adaptation in Wild Barley at Evolution Slope (Tabigha)

et al. 2021

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At the microsite "Evolution Slope", Tabigha, Israel, wild barley (Hordeum spontaneum) populations adapted to dry Terra Rossa soil, and its derivative abutting wild barley population adapted to moist and fungi-rich Basalt soil. However, the mechanisms underlying the edaphic adaptation remain elusive. Accordingly, whole genome bisulfite sequencing, RNA-sequencing, and metabolome analysis are performed on ten wild barley accessions inhabiting Terra Rossa and Basalt soil. A total of 121 433 differentially methylated regions (DMRs) and 10 478 DMR-genes are identified between the two wild barley populations. DMR-genes in CG context (CG-DMR-genes) are enriched in the pathways related with the fundamental processes, and DMR-genes in CHH context (CHH-DMR-genes) are mainly associated with defense response. Transcriptome and metabolome analysis reveal that the primary and secondary metabolisms are more active in Terra Rossa and Basalt wild barley populations, respectively. Multi-omics analysis indicate that sugar metabolism facilitates the adaptation of wild barley to dry Terra Rossa soil, whereas the enhancement of phenylpropanoid/phenolamide biosynthesis is beneficial for wild barley to inhabit moist and fungi pathogen-rich Basalt soil. The current results make a deep insight into edaphic adaptation of wild barley and provide elite genetic and epigenetic resources for developing barley with high abiotic stress tolerance.

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Section: Introductionmentioning

confidence: 99%

Multi‐Omics Analysis Reveals the Mechanism Underlying the Edaphic Adaptation in Wild Barley at Evolution Slope (Tabigha)

et al. 2021

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“…The pipecolate, a lysine-derived non-protein heterocyclic amino acid, increased under drought stress. Pipecolate significantly increased under osmotic stresses, metal stresses and nutrient deficiencies, indicating pipecolic acid plays a vital role in plant systemic acquired counteraction against biotic elicitors ( Zhao et al, 2021 ). Threonine, methionine and acetyl-homoserine, three derivatives of homoserine, also increased under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Combining Metabolic Analysis With Biological Endpoints Provides a View Into the Drought Resistance Mechanism of Carex breviculmis

Liu

et al. 2022

Front. Plant Sci.

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Metabolomics is an effective tool to test the response of plants to environmental stress; however, the relationships between metabolites and biological endpoints remained obscure in response to drought stress. Carex breviculmis is widely used in forage production, turf management, and landscape application and it is particularly resistant to drought stress. We investigated the metabolomic responses of C. breviculmis to drought stress by imposing a 22-day natural soil water loss. The results showed that water-deficit restrained plant growth, reducing plant height, leaf fresh weight, and total weight, however, increasing soluble protein content and malondialdehyde content. In total, 129 differential metabolites in the leaves were detected between drought and control using the Ultrahigh Performance Liquid Chromatography-Mass Spectrometer (UPLC-MS) method. Drought enhanced most of the primary and secondary metabolites in the differential metabolites. Almost all the sugars, amino acids, organic acids, phytohormones, nucleotides, phenylpropanoids and polyketides in the differential metabolites were negatively correlated with plant height and leaf fresh weight, while they were positively correlated with soluble protein content and malondialdehyde content. Metabolic pathway analysis showed that drought stress significantly affected aminoacyl-tRNA biosynthesis, TCA cycling, starch and sucrose metabolism. Our study is the first statement on metabolomic responses to drought stress in the drought-enduring plant C. breviculmis. According to the result, the coordination between diverse metabolic pathways in C. breviculmis enables the plant to adapt to a drought environment. This study will provide a systematic framework for explaining the metabolic plasticity and drought tolerance mechanisms of C. breviculmis under drought stress.

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“…Lysine metabolite Pip is related to SA in plant disease defense. Pip in plants exposed to all abiotic stresses changed significantly compared with the control [ 53 ], but little is known about its role in drought stress. In this study, the deletion of tomato Pip biosynthetic gene SlALD1 showed drought resistance, and the hydroxylated modification gene SlFMO1 deletion showed sensitivity to drought.…”

Section: Discussionmentioning

confidence: 99%

A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato

et al. 2021

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With global warming and water shortage, drought stress is provoking an increasing impact on plant growth, development, and crop productivity worldwide. Pipecolic acid (Pip) is an emerging lysine catabolite in plants, acting as a critical element in disease resistance with a related signal pathway of phytohormone salicylic acid (SA). While SA plays a vital role in various abiotic stresses, the role of Pip in plant response to abiotic stresses, especially drought, remains largely unknown. To address this issue, Pip biosynthetic gene Slald1 mutants and hydroxylated modification gene Slfmo1 mutants were generated using CRISPR-Cas9 gene-editing approaches. Drought resistance dramatically increased in Slald1 mutants compared with wild-type, which was associated with increased CO2 assimilation, photosystems activities, antioxidant enzymes activities, ascorbate and glutathione content, and reduced reactive oxygen species accumulation, lipid peroxidation and protein oxidation. On the contrary, Slfmo1 mutants were more sensitive to drought, showing damaged photosystems and impaired antioxidant systems, which were significantly alleviated by exogenous ascorbate. Our results demonstrate that Pip biosynthesis and hydroxylated modification pathways play a critical role in drought tolerance through the antioxidant system in tomato. This knowledge can be helpful to breed improved crop cultivars that are better equipped with drought resistance.

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Comprehensive dissection of primary metabolites in response to diverse abiotic stress in barley at seedling stage

Cited by 22 publications

References 43 publications

Multi‐Omics Analysis Reveals the Mechanism Underlying the Edaphic Adaptation in Wild Barley at Evolution Slope (Tabigha)

Multi‐Omics Analysis Reveals the Mechanism Underlying the Edaphic Adaptation in Wild Barley at Evolution Slope (Tabigha)

Combining Metabolic Analysis With Biological Endpoints Provides a View Into the Drought Resistance Mechanism of Carex breviculmis

A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato

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