A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato

Wang, Ping; Luo, Qian; Yang, Wei-Cheng; Ahammed, Golam Jalal; Ding, Shuting; Chen, Xingyu; Wang, Jiao; Xia, Xiaojian; Shi, Kai

doi:10.3390/antiox10121923

Cited by 21 publications

(17 citation statements)

References 58 publications

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“…The resulting supernatant was then used to measure enzymatic activity with a UV-2410PC spectrophotometer (Shimadzu, Japan). The activities of SOD, POD, CAT, and ascorbate peroxidase were measured according to the previously described protocol [ 47 ]. MDAR [ 48 ] and GST [ 49 ] enzymatic activities were measured following previous protocols.…”

Section: Methodsmentioning

confidence: 99%

HsfA1a confers pollen thermotolerance through upregulating antioxidant capacity, protein repair, and degradation in Solanum lycopersicum L.

Dan

Huang

Zhou

et al. 2022

Horticulture Research

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The heat shock transcription factors (Hsfs) play critical roles in plant responses to abiotic stresses. However, the mechanism of Hsfs in the regulation of pollen thermotolerance and their specific biological functions and signaling remain unclear. Herein, we demonstrated that HsfA1a played a key role in tomato pollen thermotolerance. hsfA1a mutants reduced, while its overexpression increased pollen themotolerance based on the pollen viability and germination. Analyzing the whole transcriptome by RNA-Seq data, we found that HsfA1a mainly regulated the genes involved in oxidative stress protection, protein homeostasis regulation and protein modification, as well as the response to biological stress in anthers under heat stress. The accumulation of reactive oxygen species in anthers were enhanced in hsfA1a mutants, while were decreased in HsfA1a overexpressing lines. Furthermore, the bounding of HsfA1a to the promoter region of genes involved in redox regulation (Cu/Zn-SOD, GST8 and MDAR1), protein repair (HSP17.6A, HSP70–2, HSP90–2 and HSP101) and degradation (UBP5, UBP18, RPN10a and ATG10) and regulated the expression of these genes in tomato anthers under heat stress. Our findings suggest that HsfA1a maintains pollen thermotolerance and cellular homeostasis by enhancing the antioxidant capacity and protein repair and degradation, ultimately improving pollen viability and fertility.

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Section: Methodsmentioning

confidence: 99%

HsfA1a confers pollen thermotolerance through upregulating antioxidant capacity, protein repair, and degradation in Solanum lycopersicum L.

Dan

Huang

Zhou

et al. 2022

Horticulture Research

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“…Pipecolic acid is a lysine−derived non−protein amino acid which regulates plant systemic acquired resistance and basal immunity to bacterial pathogen infection [ 39 ]. Recent study has shown that pipecolic acid plays a negative regulatory role in drought tolerance of tomato plants [ 40 ]. In this study, the level of DL−pipecolic acid in DT decreased under mild drought stress, which may be related to the drought resistance response in DT.…”

Section: Discussionmentioning

confidence: 99%

Comparative Metabolic Study of Two Contrasting Chinese Cabbage Genotypes under Mild and Severe Drought Stress

Chen

Shen

Yang

et al. 2022

IJMS

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Chinese cabbage (Brassica rapa L. ssp. pekinensis) is an important leafy vegetable crop cultivated worldwide. Drought is one of the most important limiting factors for the growth, production and quality of Chinese cabbage due to its weak drought tolerance. In order to deepen the understanding of drought stress response in Chinese cabbage, metabolomics studies were conducted in drought−tolerant (DT) and drought−susceptible (DS) genotypes of Chinese cabbage under water deficit−simulated mild and severe drought stress conditions. A total of 777 metabolites were detected, wherein 90 of them were proposed as the drought−responsive metabolites in Chinese cabbage, with abscisic acid (ABA), serine, choline alfoscerate, and sphingosine as potential representative drought stress biomarkers. We also found that drought−tolerant and drought−susceptible genotypes showed differential metabolic accumulation patterns with contrasting drought response mechanisms. Notably, constitutively high levels of ABA and glutathione were detected in drought−tolerant genotype in all tested and control conditions. In addition, proline, sucrose, γ−aminobutyric acid, and glutathione were also found to be highly correlated to drought tolerance. This study is the first metabolomic study on how Chinese cabbage responds to drought stress, and could provide insights on how to develop and cultivate new drought−resistant varieties.

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“…Antioxidants in plants have been classified into two main types: enzymatic and nonenzymatic antioxidants. The significant antioxidant enzymes in plant cells contain SOD, CAT, POD, and so on [ 63 ]. GSH and AsA are vital nonenzymatic antioxidants in plants.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, APX, DHAR, and GR serve as significant enzymes in the AsA-GSH cycle [ 64 ]. Antioxidants are involved in multiple plant abiotic stresses, including heat stress [ 63 ]. Treating seedlings of Broussonetia papyrifera at high temperature, the activities of SOD, POD, and CAT were significantly increased [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum

et al. 2022

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The Bcl-2-associated athanogene (BAG) family, a group of co-chaperones that share conservative domains in flora and fauna, is involved in plant growth, development, and stress tolerance. However, the function of tomato BAG genes on thermotolerance remains largely unknown. Herein, we found that the expression of BAG9 was induced during heat stress in tomato plants. Knockout of the BAG9 gene by CRISPR/Cas9 reduced, while its overexpression increased thermotolerance in tomato plants as reflected by the phenotype, photosynthesis rate, and membrane peroxidation. Heat-induced reactive oxygen species and oxidative/oxidized proteins were further increased in bag9 mutants and were normalized in BAG9 overexpressing plants. Furthermore, the activities of antioxidant enzymes, ascorbic acid (AsA)/dehydroascorbic acid (DHA), and reduced glutathione (GSH)/oxidized glutathione (GSSG) were reduced in bag9 mutants and were increased in BAG9 overexpressing plants under heat stress. Additionally, BAG9 interacted with Hsp20 proteins in vitro and in vivo. Accumulation of Hsp proteins induced by heat showed a reduction in bag9 mutants; meanwhile, it was increased in BAG9 overexpressing plants. Thus, BAG9 played a crucial role in response to heat stress by regulating cellular redox homeostasis and the stability of heat shock proteins.

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A Novel Role of Pipecolic Acid Biosynthetic Pathway in Drought Tolerance through the Antioxidant System in Tomato

Cited by 21 publications

References 58 publications

HsfA1a confers pollen thermotolerance through upregulating antioxidant capacity, protein repair, and degradation in Solanum lycopersicum L.

HsfA1a confers pollen thermotolerance through upregulating antioxidant capacity, protein repair, and degradation in Solanum lycopersicum L.

Comparative Metabolic Study of Two Contrasting Chinese Cabbage Genotypes under Mild and Severe Drought Stress

BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum

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