Comprehensive approach for the detection of antifungal compounds using a susceptible strain of Candida albicans and confirmation of in vivo activity with the Galleria mellonella model

Favre-Godal, Quentin; Dorsaz, Stéphane; Queiroz, Emerson Ferreira; Conan, Céline; Marcourt, Laurence; Wardojo, Bambang Prajogo Eko; Voinesco, Francine; Buchwalder, Aurélie; Gindro, Katia; Sanglard, Dominique; Wolfender, Jean‐Luc

doi:10.1016/j.phytochem.2014.06.004

Cited by 38 publications

(62 citation statements)

References 43 publications

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“…12 In order to identify rapidly antifungals from plant sources, we have recently developed a strategy for the early localization and efficient identification of antifungal compounds in complex crude extracts. 13 Using this approach more than 150 species and 400 extracts with different polarities have been evaluated. Among all extracts, those of three plants that exhibited significant activities against a susceptible strain of C. albicans were selected.…”

Section: Introductionmentioning

confidence: 99%

“…This C. albicans isolate is used as a sensitive tool to detect bioactive secondary metabolites. 13 These plants (Schefflera systyla (Donn. Sm.)…”

Section: Introductionmentioning

confidence: 99%

“…To calculate the MIQ, 10 µL aliquots of different concentrations (from 0.01 to 10 mg mL -1 in MeOH) of the pure compounds were spotted manually on the HPTLC plate as well as 10 µL of only MeOH. Then, the HPTLC plate without elution was submitted to the same procedure published by Favre-Godal et al 13 The MIQ was defined as the test compound quantity at which inhibition was observed. …”

mentioning

confidence: 99%

“…13 Using this approach more than 150 species and 400 extracts with different polarities have been evaluated. Among all extracts, those of three plants that exhibited significant activities against a susceptible strain of C. albicans were selected.…”

mentioning

confidence: 99%

“…13 This in vivo model is used to study the virulence of microbial pathogens and the efficacy of antimicrobial agents using insect larvae. Several studies have demonstrated the efficacy of known antifungal agents in this model for Candida infection.…”

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confidence: 99%

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Identification of Triterpenoids from Schefflera systyla, Odontadenia puncticulosa and Conostegia speciosa and In Depth Investigation of Their in vitro and in vivo Antifungal Activities

Favre-Godal¹,

Dorsaz²,

Marcourt³

et al. 2016

J. Braz. Chem. Soc.

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As a part of a broad screening of antifungal agents from plant origin, crude extracts from Panamanian plants having related types of constituents displayed significant activities in an agar overlay thin layer chromatography assay against a susceptible strain of Candida albicans. These were the methanolic extract of the leaves of Schefflera systyla and Odontadenia puncticulosa and of the stems of Conostegia speciosa, that are species not previously investigated from a phytochemical viewpoint. For all plants, high-performance liquid chromatography (HPLC) antifungal activity based profiling allowed the rapid localization of antifungal agents that were further obtained by targeted isolation procedure by semi-preparative HPLC or medium pressure liquid chromatography (MPLC) after LC gradient transfer. Different hederagenin saponins and one aglycone were found to be responsible for the antifungal activities of the extracts. Alpha-hederin was the antifungal of S. systyla, pulsatilla saponin D and 3β-O-[β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabinopyranosylhederagenin of O. puncticulosa and arjunolic acid of C. speciosa. Their minimal inhibitory concentration (MIC) against planktonic and biofilm cells of C. albicans were determined. Alpha-hederin was the most potent compound with a MIC of 4 µg mL -1 . Structurally related compounds (hederagenin, medicagenic acid 3-O-β-D-glucopyranoside and medicagenic acid) were used as standards and tested for comparison purposes. In order to better estimate the potential of these triterpenoids as antifungal agents, their cytological effects on C. albicans were determined by transmission electron microscopy (TEM) and the in vivo activity of alpha-hederin, medicagenic acid 3-O-β-D-glucopyranoside and medicagenic acid was evaluated for the first time in the Galleria mellonella larvae model. Keywords: triterpenoids, microfractionation, antifungal, Candida species, Galleria mellonella IntroductionFungal infections have recently increased worldwide and cause high morbidity and mortality rates among different groups of human patients. 1 The development of drug resistance in fungal pathogens compromises the efficacy of the limited number of therapeutic agents. 2The main fungal opportunistic human pathogens are Candida spp., and among them, the most common species is Candida albicans. 3 In the USA, Candida species are the fourth cause of nosocomial bloodstream infections. 4,5 Over 400,000 candidiasis infections are declared per year worldwide. 6 Moreover, Candida species display a tendency Identification of Triterpenoids from Schefflera systyla, Odontadenia puncticulosa and Conostegia speciosa J. Braz. Chem. Soc. 444 to grow as biofilms on implanted medical devices such as a central venous catheter. 7 Biofilm cells exhibit up to a 1,000-fold increase in resistance as compared to their planktonic counterparts 8 which may contribute to therapeutic failures. 9 These facts make urgent the need to identify new chemical entities that may lead to the disc...

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Section: Introductionmentioning

confidence: 99%

“…This C. albicans isolate is used as a sensitive tool to detect bioactive secondary metabolites. 13 These plants (Schefflera systyla (Donn. Sm.)…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of Triterpenoids from Schefflera systyla, Odontadenia puncticulosa and Conostegia speciosa and In Depth Investigation of Their in vitro and in vivo Antifungal Activities

Favre-Godal¹,

Dorsaz²,

Marcourt³

et al. 2016

J. Braz. Chem. Soc.

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New Prenylated ortho‐Dihydroxycoumarins from the Fruits of Ficus nipponica

Akihara

Ohta

Nehira

et al. 2017

Chemistry & Biodiversity

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Two new prenylated ortho-dihydroxycoumarins, designated fipsomin (1) and fipsotwin (2), were isolated from the fruits of Ficus nipponica together with a known prenylated coumarin, apigravin (3). Their structures were established by spectroscopic data and X-ray crystallographic analysis. Compound 1 exhibited antibacterial activity against Bacillus subtilis with an MIC value of 61 μm, while 2 and 3 showed very weak activity.

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Natural Antifungal Products: Another Option for Antifungal Resistance

Hernández‐Castro

Xicohtencatl‐Cortes

2022

Fungal Biology

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Comprehensive approach for the detection of antifungal compounds using a susceptible strain of Candida albicans and confirmation of in vivo activity with the Galleria mellonella model

Cited by 38 publications

References 43 publications

Identification of Triterpenoids from Schefflera systyla, Odontadenia puncticulosa and Conostegia speciosa and In Depth Investigation of Their in vitro and in vivo Antifungal Activities

Identification of Triterpenoids from Schefflera systyla, Odontadenia puncticulosa and Conostegia speciosa and In Depth Investigation of Their in vitro and in vivo Antifungal Activities

New Prenylated ortho‐Dihydroxycoumarins from the Fruits of Ficus nipponica

Natural Antifungal Products: Another Option for Antifungal Resistance

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