Comprehensive analysis of the Gossypium hirsutum L. respiratory burst oxidase homolog (Ghrboh) gene family

Abstract

Background: Plant NADPH oxidase (NOX), also known as respiratory burst oxidase homolog (rboh), encoded by the rboh gene, is a key enzyme in the reactive oxygen species (ROS) metabolic network. It catalyzes the formation of the superoxide anion (O 2 •−), a type of ROS. In recent years, various studies had shown that members of the plant rboh gene family were involved in plant growth and developmental processes as well as in biotic and abiotic stress responses, but little is known about its functional role in upland cotton. Results: In the present study, 26 putative Ghrboh genes were identified and characterized. They were phylogenetically classified into six subfamilies and distributed at different densities across 18 of the 26 chromosomes or scaffolds. Their exon-intron structures, conserved domains, synteny and collinearity, gene family evolution, regulation mediated by cisacting elements and microRNAs (miRNAs) were predicted and analyzed. Additionally, expression profiles of Ghrboh gene family were analyzed in different tissues/organs and at different developmental stages and under different abiotic stresses, using RNA-Seq data and real-time PCR. These profiling studies indicated that the Ghrboh genes exhibited temporal and spatial specificity with respect to expression, and might play important roles in cotton development and in stress tolerance through modulating NOX-dependent ROS induction and other signaling pathways. Conclusions: This comprehensive analysis of the characteristics of the Ghrboh gene family determined features such as sequence, synteny and collinearity, phylogenetic and evolutionary relationship, expression patterns, and cis-elementand miRNA-mediated regulation of gene expression. Our results will provide valuable information to help with further gene cloning, evolutionary analysis, and biological function analysis of cotton rbohs.

Comprehensive Analysis of Respiratory Burst Oxidase Homologs (Rboh) Gene Family and Function of GbRboh5/18 on Verticillium Wilt Resistance in Gossypium barbadense

“…Both G. arboretum and G. raimondii harbored 13 Rboh genes, a result that agrees with the findings of Zhang et al (2020). The main difference with that work is that we found 24 and 27 Rboh genes in G. hirsutum and G. barbadense, while they found 26 and 19, respectively (Wang et al, 2020). For G. hirsutum, 4 of 26 GhRbohs were anchored to unmapped scaffolds in the NAU-NBI v1.1 assembly genome (assembled in 2015), whereas we found that all 24 GhRbohs in the HAU v1.0 assembly genome (assembled in 2018) mapped onto the A t or D t sub-genome chromosomes.…”

Identification of the conserved domains of ADP-Glucose Pyrophosphorylase (AGPase) protein in sweetpotato (Ipomoea batatas (L.) Lam.) and its two wild relatives

“…The amount of AGPases increased by whole-genome duplication in sweetpotatoes from its relatives. This result is consistent with a study showing that the number of rboh genes in the polyploid plant, Gossypium hirsutum, was higher than its progenitor plants G. raimonddi and G. arboreum (Wang et al 2020). Moreover, some AGPases in I. tri da and I. triloba exhibited an II value ≥ 40, which means an unstable state, but there was no AGPase representing an II value ≥ 40 in I. batatas (Table 1).…”

NADPH oxidases and the evolution of plant salinity tolerance

“…Chlamydomonas reinhardtii (chlorophyte alga), but not from the other three chlorophyte algae tested (Micromonas pusilla, Ostreococcus lucimarinus and Volvox carteri) (Wang et al, 2020). Previous studies also identified RBOH homologues in some red alga species, such as Cyanidioschyzon merolae .…”