Comprehensive analysis of plasmid-mediated tet(X4)-positive Escherichia coli isolates from clinical settings revealed a high correlation with animals and environments-derived strains

Cui, Chao‐Yue; Li, Xiaojie; Chen, Chong; Wu, Xiao-Ting; Qian, He; Jia, Qiu-Lin; Zhang, Xiaojing; Lin, Zhuo-Yu; Li, Cang; Fang, Liang‐Xing; Liao, Xiao‐Ping; Hu, Bo; Sun, Jian

doi:10.1016/j.scitotenv.2021.150687

Cited by 30 publications

(36 citation statements)

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“…Although horizontal transfer mediated by plasmids (e.g., IncQ, and IncX1) and insertion sequences (e.g., IS CR2 , IS 26 , and IS 1 ) is the main mechanism for tet (X4) transmission ( Aminov, 2021 ; Liu et al, 2022 ; Yu et al, 2022 ), clonal spread of tet (X4)-carrying strains, such as E. coli ST877, ST10, and ST48 clones is also responsible for tet (X4) dissemination between animals and humans ( Cui et al, 2022 ). The E. hormaechei co-harboring tet (X4) and bla NDM could also clonally spread from the slaughterhouse to the retail market ( Li et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China

Wang

et al. 2022

Front. Microbiol.

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This study aimed to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from a pig farm in Shanghai, China, and to elucidate tet(X4) dissemination mechanism in this swine farm. Forty-nine (80.33%) E. coli strains were isolated from 61 samples from a pig farm and were screened for the presence of tet(X). Among them, six (12.24%) strains were positive for tet(X4) and exhibited resistance to tigecycline (MIC ≥ 16 mg/L). They were further sequenced by Illumina Hiseq. Six tet(X4)-positive strains belonged to ST761 with identical resistance genes, resistance profiles, plasmid replicons, and cgMLST type except that additional ColE10 plasmid was present in isolate SH21PTE35. Isolate SH21PTE31, as a representative ST761 E. coli strain, was further sequenced using Nanopore MinION. The tet(X4) in SH21PTE31 was located on IncFIA18/IncFIB(K)/IncX1 hybrid plasmid pYUSHP31-1, highly similar to other tet(X4)-carrying IncFIA18/IncFIB(K)/IncX1 plasmids from ST761 E. coli and other E. coli lineages in China. These IncFIA18/IncFIB(K)/IncX1 plasmids shared closely related multidrug resistance regions, and could reorganize, acquire or lose resistance modules mediated by mobile elements such as ISCR2 and IS26. Phylogenetic analysis were performed including all tet(X4)-positive isolates obtained in this pig farm combined with 43 tet(X4)-positive E. coli from pigs, cow, pork, wastewater, and patients with the same ST from NCBI. The 50 tet(X4)-carrying E. coli ST761 isolates from different areas in China shared a close phylogenetic relationship (0-49 SNPs). In conclusion, clonal transmission of tet(X4)-positive E. coli ST761 has occurred in this swine farm. E. coli ST761 has the potential to become a high-risk clone for tet(X4) dissemination in China.

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Section: Resultsmentioning

confidence: 99%

Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China

Wang

et al. 2022

Front. Microbiol.

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“…Structurally, Tet(X) comprises a substrate-binding domain, coenzyme flavin adenine dinucleotide (FAD)-binding domain and C-terminal α-helix. Site-directed mutagenesis confirmed that the resistance phenotype/activity (capacity to mediate low-, moderate-, or high-level TIG resistance) of tet (X) variants is dependent on the residues in the TETs binding region and sites in the FAD binding region ( Cui et al, 2021a , b , c ). Thus, the accumulation of beneficial mutations results in increased activity (ability to exert moderate or HLT resistance) of tet (X) variants ( Cui et al, 2021a ).…”

Section: Mechanism Of Tigecycline Resistancementioning

confidence: 89%

Mobile Tigecycline Resistance: An Emerging Health Catastrophe Requiring Urgent One Health Global Intervention

et al. 2022

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Mobile tigecycline resistance (MTR) threatens the clinical efficacy of the salvage antibiotic, tigecycline (TIG) used in treating deadly infections in humans caused by superbugs (multidrug-, extensively drug-, and pandrug-resistant bacteria), including carbapenem- and colistin-resistant bacteria. Currently, non-mobile tet(X) and mobile plasmid-mediated transmissible tet(X) and resistance-nodulation-division (RND) efflux pump tmexCD-toprJ genes, conferring high-level TIG (HLT) resistance have been detected in humans, animals, and environmental ecosystems. Given the increasing rate of development and spread of plasmid-mediated resistance against the two last-resort antibiotics, colistin (COL) and TIG, there is a need to alert the global community on the emergence and spread of plasmid-mediated HLT resistance and the need for nations, especially developing countries, to increase their antimicrobial stewardship. Justifiably, MTR spread projects One Health ramifications and portends a monumental threat to global public and animal health, which could lead to outrageous health and economic impact due to limited options for therapy. To delve more into this very important subject matter, this current work will discuss why MTR is an emerging health catastrophe requiring urgent One Health global intervention, which has been constructed as follows: (a) antimicrobial activity of TIG; (b) mechanism of TIG resistance; (c) distribution, reservoirs, and traits of MTR gene-harboring isolates; (d) causes of MTR development; (e) possible MTR gene transfer mode and One Health implication; and (f) MTR spread and mitigating strategies.

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“…In previous studies, the IncX1-type plasmid was a common vector for tet (X4), which has a strong transmission ability and a wide host range [ 6 , 8 ]. It was interesting that no IncX1 replicon gene was found in this study, but that the IncX4 (4.5%) and IncX3 (1.8%) plasmid replicon genes were identified.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, tet (X4)-producing E. coli isolates have been disseminated in meat products such as pork and chicken, food animals, animal feces, and farm soil [ 3 , 4 , 5 , 6 , 7 ]. In a previous study, tet (X4)-producing E. coli isolates in pigs and chickens accounted for 1.3% and 0.8%, respectively, which was disseminated in five provinces in southern and eastern China including Guangdong, Fujian, Jiangsu, Jiangxi, and Guangxi [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…These results suggested that food animals and their meat products were still an important reservoir of tet (X4). It was noted that tet (X4)-producing E. coli isolates also emerged in humans in recent years, where the detection rate of tet (X4)-producing E. coli isolates from hospital patients was 4.5% [ 6 ]. The genetic link between meat/animal-borne tet (X4)-producing E. coli isolates and human-borne isolates requires urgent investigation.…”

Section: Introductionmentioning

confidence: 99%

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International Spread of Tet(X4)-Producing Escherichia coli Isolates

Zhang

Zhan

Shi

2022

Foods

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Tigecycline resistance in bacteria has become a significant threat to food safety and public health, where the development of which is attributed to plasmid-mediated tet(X4) genes. In this study, the genomes of 613 tet(X4)-producing Escherichia coli (E. coli) isolates, available from public databases, are evaluated to determine their international prevalence and molecular characterization. These E. coli isolates have been disseminated in 12 countries across Asia and Europe. It was found that pigs and their products (n = 162) were the most common vehicle, followed by humans (n = 122), chickens (n = 60), and the environment (n = 49). Carbapenems-resistant genes blaNDM-5 (1.3%) and blaNDM-1 (0.2%) were identified, as well as colistin-resistant genes mcr-1.1 (12.6%) and mcr-3.1 (0.5%). It was noted that the tigecycline-resistant gene cluster tmexC-tmexD-toprJ1 was identified in seven (1.1%) isolates. Phylogenomic results indicated that tet(X4)-producing E. coli isolates fell into seven lineages (lineages I, II, III, IV, V, VI, and VII), and international spread mainly occurred in Asian countries, especially China, Pakistan, Singapore, and Malaysia. Four forms of tet(X4) transposon units were found, including the I-type (IS26-tet(X4)-ISCR2), II-type (ΔIS1R-tet(X4)-ISCR2), III-type (ISCR2-tet(X4)-ISCR2), and IV-type (ISCR2-tet(X4)-ΔISCR2). These findings underline further challenges for the spread of E. coli bearing tet(X4) gene.

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Comprehensive analysis of plasmid-mediated tet(X4)-positive Escherichia coli isolates from clinical settings revealed a high correlation with animals and environments-derived strains

Cited by 30 publications

References 35 publications

Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China

Tigecycline-resistant Escherichia coli ST761 carrying tet(X4) in a pig farm, China

Mobile Tigecycline Resistance: An Emerging Health Catastrophe Requiring Urgent One Health Global Intervention

International Spread of Tet(X4)-Producing Escherichia coli Isolates

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