Comprehension of the zinc chloride's ameliorative apoptotic and genotoxic effects on mice with cadmium-induced hepatotoxicity

Bayram, Enas Nihad; Al-Shmgani, Hanady S.; Al-Bakri, Nahla A.

doi:10.31893/jabb.22035

Cited by 2 publications

(3 citation statements)

References 26 publications

(27 reference statements)

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“…This change in expression in Bcl-2 simultaneously activates caspase-3 and triggers apoptotic processes [ 26 ]. A significant increase in the caspase-3 protein level has been highlighted and reported in our previous study in mice treated with cadmium chloride, which resulted in increased apoptotic hepatocytes [ 8 ]. Moreover, the expression of apoptotic proteins is associated with increased levels of free radicals.…”

Section: Discussionmentioning

confidence: 79%

“…This is because both the liver and kidneys have the capacity to produce metallothionein proteins. In our previous study, we reported that cadmium toxicity interferes with vital metals (zinc), induces oxidative stress, increases DNA strand damage, and triggers an active inflammatory response in the liver tissue [ 8 ]. Histological analysis is a critical and sensitive measure in identifying potential cellular alterations in the target organs exposed to heavy metals in order to determine its tissue pathology.…”

Section: Introductionmentioning

confidence: 99%

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Zinc Chloride Can Mitigate the Alterations in Metallothionein and Some Apoptotic Proteins Induced by Cadmium Chloride in Mice Hepatocytes: A Histological and Immunohistochemical Study

Bayram

Al-Bakri

Al-Shmgani

2023

Journal of Toxicology

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The heavy metal cadmium is extremely harmful to both humans and animals. Zinc supplementation protects the biological system and reduces cadmium-induced toxicity. This study aimed to determine whether zinc chloride (ZnCl2) could protect male mice with the damaged liver induced by cadmium chloride (CdCl2). The protective role of zinc chloride and expression of the metallothionein (MT), Ki-67, and Bcl-2 apoptotic proteins in hepatocytes were studied after subchronic exposure of mice to cadmium chloride for 21 days. Thirty male mice were randomly categorized into 6 groups (5 mice/group) as follows: a control group that did not receive any treatment, a group given ZnCl2 at 10 mg/kg alone, and two groups received ZnCl2 (10 mg/kg) in combination with CdCl2 at two concentrations (1.5 and 3 mg/kg), while the last two groups received CdCl2 alone at 1.5 and 3 mg/kg, respectively. Immunohistochemical examination revealed a decrease in Ki-67 expression in Kupffer and endothelial cells, which reflected cell proliferation downregulation accompanied by MT increased expression. However, the Bcl-2 was ameliorated and reduced to demonstrate an enhanced rate of necrosis rather than apoptosis. Furthermore, histopathological results showed significant alteration such as hepatocytes with a pyknotic nucleus, infiltration of inflammatory cells around the central vein, and the presence of many binucleated hepatocytes. Zinc chloride treatment resulted in histological and morphological improvements that were average in the expression of apoptosis proteins modifications induced by cadmium. Our findings revealed that the positive effects of zinc might be linked to the high metallothionein expression and enhanced cell proliferation. Furthermore, at low-dose exposure, cadmium-induced damage to cells could be more closely related to necrosis rather than apoptosis.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Zinc Chloride Can Mitigate the Alterations in Metallothionein and Some Apoptotic Proteins Induced by Cadmium Chloride in Mice Hepatocytes: A Histological and Immunohistochemical Study

Bayram

Al-Bakri

Al-Shmgani

2023

Journal of Toxicology

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“…Female albino rats, ranged in age between 14 to 16 weeks, with an average was of 225 ± 5 gm. The rats were put in the plastic cages breeding with metal lids and Brush the cage with sawdust, environmental conditions controlled at the constant Photoperiod (12 hour day /12 hour night) cycle ventilation, temperature ranged between 20-24 c. Animals were given a sufficient amount of water and food from a local source (Wheat 34% , barley 20% ,corn 25% ,animal protein 10% , powdered milk 10% ,salt 1% ) these ingredient were grinded and mixed with some oil and water until they become a paste coherent (14,15,16). And put in the designated place for the food in the cages for animal breeding, two mature females were caged together with one mature male overnight and in the following morning the females were checked for the presence of vaginal plug (17).…”

Section: Sample Collectionmentioning

confidence: 99%

Teratoma induced by amlodipine drug in fetus rats

Dumue Qasim Mohammed,

Ali Naeem Salman,

Nahla A. Al-Bakri

2023

GSC Adv. Res. Rev.

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The current study, performed during the period from February 2021 to June 2022 at the University of Thi- Qar/ College of Education for Pure Sciences, and aimed to follow the changes in external morphological features at different Embryonic developmental stages in pregnant rats treated with different doses of amlodipine. Usage In this study, 18 pregnant rats were randomly divided into three groups, each with six pregnant rats. Each group received different concentrations of amlodipine (0.3, 0.5) in oral doses until the 20th day of gestation, while the control group was injected with 0.9% normal saline. Teratomas are bizarre tumors derived from embryonic tissue that are normally found only in the gonadal and sacral regions of adults. Primary retroperitoneal teratomas are rare and present challenging management options. In one outcome, a teratoma was found in one of the amlodipine-treated groups. Complete excision of the teratoma mass was performed.

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Comprehension of the zinc chloride's ameliorative apoptotic and genotoxic effects on mice with cadmium-induced hepatotoxicity

Cited by 2 publications

References 26 publications

Zinc Chloride Can Mitigate the Alterations in Metallothionein and Some Apoptotic Proteins Induced by Cadmium Chloride in Mice Hepatocytes: A Histological and Immunohistochemical Study

Zinc Chloride Can Mitigate the Alterations in Metallothionein and Some Apoptotic Proteins Induced by Cadmium Chloride in Mice Hepatocytes: A Histological and Immunohistochemical Study

Teratoma induced by amlodipine drug in fetus rats

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