Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line

Mutiah, Roihatul; Minggarwati, Trian Sidha; Kristanti, Risma Aprinda; Susanti, Erna

doi:10.14499/indonesianjcanchemoprev10iss3pp131-139

Cited by 11 publications

(13 citation statements)

References 12 publications

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“…Natural ingredients proven to have anticancer properties include resveratrol. [ 12 13 ] Our findings showed that resveratrol had good bioavailability and interacted with genes associated with lung cancer. Consistently, resveratrol might be involved in the biological process of lung cancer either the P53 signaling pathway (Hsa04115), MicroRNAs in cancer (Hsa05206), and Apoptosis (Hsa04210) either in small-cell lung cancer (Hsa05222) or nonsmall-cell lung cancer (Hsa05223) from network pharmacology followed by PPI network analysis.…”

Section: Discussionmentioning

confidence: 84%

Exploring the anticancer potential of Eleutherine bulbosa: A systematic network pharmacology study on lung cancer

Mutiah,

Rachmawati

2024

Journal of Advanced Pharmaceutical Technology &Amp; Research

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Chemotherapy application in lung cancer patients has several side effects and shows lower effectiveness due to chemoresistance. Although Eleutherine bulbosa (Mill.) Urb. (EBE) elicit anticancer properties, yet the exact profile of its active compounds and lung cancer inhibition mechanisms were not fully understood. This study aimed to identify suggestive compounds from EBE extract and explain the molecular mechanisms of EBE against lung cancer. Identification of the compound from the EBE extract was confirmed using liquid chromatography–tandem mass spectrophotometry (LC–MS/MS). The bioavailability profile of three major metabolites was identified using absorption, distribution, metabolism, excretion, toxicity software. The anticancer molecular mechanism prediction of the drugs was ascertained by network pharmacology using Cytoscape 3.9.1 and the protein–protein interaction network technique with STRING 11.0. Interaction between resveratrol and extracellular growth factor receptor (EGFR) was analyzed using site-specific molecular docking with erlotinib as the control using PyRx Autodock Vina 9.0 and BIOVIA Discovery Studio. A total of 16 active compounds were identified from LC-MS/MS. Only resveratrol showed anticancer properties by its interaction with 13 genes and 6 signaling pathways related to lung cancer. The molecular docking result supports the network pharmacology finding. The binding affinity of resveratrol with EGFR, important receptor in lung cancer, was more negative (−6.9 kcal/mol) than erlotinib (−6.2 kcal/mol) as the control. Evidence suggested that resveratrol in EBE exhibits anticancer effects by modulating lung cancer cell proliferation and apoptosis through EGFR binding.

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Section: Discussionmentioning

confidence: 84%

Exploring the anticancer potential of Eleutherine bulbosa: A systematic network pharmacology study on lung cancer

Mutiah,

Rachmawati

2024

Journal of Advanced Pharmaceutical Technology &Amp; Research

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“…Fluorescence intensity was measured using the FL-1H parameter to detect FITC. Subsequently, the percentage of apoptosis was analyzed using the Cell Quest software (BD Bioscience) [19].…”

Section: Apoptosis Assaymentioning

confidence: 99%

Network Pharmacology, Apoptosis, and Cell Cycle Inhibition of Sesquiterpene Compounds From Qusthul Hindi Root Extract (Saussurea Lappa) in Breast Cancer: An in Silico and in Vitro Approach

MUTIAH,

SUKARDIMAN,

MILLIANA

et al. 2023

Int J App Pharm

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Objective: The objective of this study was to evaluate the potential and mechanisms of compounds in Qusthul Hindi extract in inhibiting proliferation, cell cycle, and inducing cell death in breast cancer through a network pharmacology approach, in silico validation, and in vitro experiments. Methods: This research employed a literature review approach to identify anti-cancer compounds and utilized a network pharmacology approach to predict the mechanisms of action of the compounds. Insilico docking was performed on the HER2 receptor (PDB: 3PP0) using Molegro Virtual Docker 6.0. Furthermore, the MTT method was used to evaluate the cytotoxic effects of Qusthul Hindi extract on T47D cells, and Flow cytometry was employed to determine the effects of the extract on apoptosis and cell cycle. Results: The network pharmacology analysis revealed that Qusthul Hindi interacted with 66 genes related to breast cancer. Pathway analysis showed a close association between Qusthul Hindi and important signaling pathways such as P53, MAPK, PI3K-Akt, and the cell cycle. Molecular docking results showed better affinity of Saussureamine B and C towards the HER2 receptor compared to trastuzumab. In vitro cytotoxicity assays demonstrated the potential activity of Qusthul Hindi extract against T47D cells (IC50: 32.81 µg/ml). Qusthul Hindi also effectively induced apoptosis in breast cancer cells with a high percentage (85.3%), and inhibited the cell cycle by reducing the G2-M and S phases. Statistical analysis revealed significant differences between the Qusthul Hindi treatment group and the control group in terms of apoptotic cell count (p<0.001). Conclusion: These findings suggest that Qusthul Hindi has potential for development as an anti-cancer agent through the inhibition of proliferation, induction of apoptosis, and cell cycle inhibition in breast cancer.

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“…In addition, the research also found the compound content, namely the group of naphthoquinone (C35H36O14) with a high percentage of area. Not only that, the analysis of the content of naphthoquinone and its derivatives has also been proven using UHPLC [2,3]. The SNEDDS formula used was the optimal formulation in the form of 50 mg / 10 ml Dayak onion extract, 10% caprylic triglycerides as oil, 1% tween 80 and 6.61% transcutol as a combination surfactant, and 12.389% propylene glycol as a co-surfactant, while the formula Dayak extract solution as a comparison used was 50 mg / 10 ml methanol p.a.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…There are many compounds possessed by Dayak onions. It also has antioxidant activity, which isoliquiritigenin (C15H11O4) included to flavonoid (chalcone) and oxyresveratrol (C14H12O4) included to polyphenol (stilbene) [2]. Other research related to the content of compounds in Dayak onion extract also states that there is a content of naphthoquinone in Dayak onion plants [3].…”

Section: Introductionmentioning

confidence: 99%

Antioxidants Activity of Self-Nanoemulsifying Drug Delivery System on Dayak Onions Extract (Eleutherine palmifolia) using DPPH (2,2 difenil-1- picrylhydrazyl) Method

Annisa

Dharma

Mutiah

et al. 2021

J. Trop. Pharm. Chem.

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Dayak onions (Eleutherine palmifolia L. Merr) is one plant that has been proven to have benefits as an antioxidant. The Dayak extract is formulated in the self nano emulsifying drug delivery system (SNEDDS) because the extract has low water solubility. The aims this study to develop the SNEDDS formulation system by testing its antioxidant activity. We determined whether there was an increase in antioxidant activity when formulated in the form of SNEDDS or not. The results were then compared with a solution of Dayak onions extract without using SNEDDS. The obtained formula was the optimal result that has been done before using the D-optimal mixture design method. The results of the components consisted of 50 mg of Dayak extract, 10 % caprylic triglyceride as oil, 1% tween 80, and 6,60 % transcutol as a combination surfactant and 12,40 % propylene glycol as co-surfactant. Antioxidant activity testing was carried out using the DPPH (1,1-Diphenyl-2-picrylhydrazyl) method. The antioxidant test using the DPPH method was done with two samples, namely the extract solution and Dayak onion extract SNEDDS. We elaborated the research by using UV-VIS spectrophotometer. Each sample was made into five concentrations, namely 30 ppm, 60 ppm, 90 ppm, 120 ppm, and 150 ppm, and carried out three times replications. The results showed that the IC50 value in the Dayak onion extract solution was 227,19 ppm (very low), while for the SNEDDS solution for the Dayak onion extract the IC50 value obtained was 38,97 ppm (very strong). The analysis was carried out next using an independent T-Test to obtain the results. There was no significant difference between the extract solution and the SNEDDS solution of Dayak onion extract.

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Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line

Cited by 11 publications

References 12 publications

Exploring the anticancer potential of Eleutherine bulbosa: A systematic network pharmacology study on lung cancer

Exploring the anticancer potential of Eleutherine bulbosa: A systematic network pharmacology study on lung cancer

Network Pharmacology, Apoptosis, and Cell Cycle Inhibition of Sesquiterpene Compounds From Qusthul Hindi Root Extract (Saussurea Lappa) in Breast Cancer: An in Silico and in Vitro Approach

Antioxidants Activity of Self-Nanoemulsifying Drug Delivery System on Dayak Onions Extract (Eleutherine palmifolia) using DPPH (2,2 difenil-1- picrylhydrazyl) Method

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