Composition of <i>Cypripedium calceolus</i> (Orchidaceae) seeds analyzed by attenuated total reflectance IR spectroscopy: In search of understanding longevity in the ground

Barsberg, Søren; Rasmussen, Henrik; Kodahl, Nete

doi:10.3732/ajb.1200646

Cited by 24 publications

(40 citation statements)

References 32 publications

(40 reference statements)

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“…, Barsberg et al . ). Very little is known about dormancy in epiphytic orchids, but Kartzinel et al .…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, almost all the accounts of dormancy (or relatively lack thereof) involve temperate, terrestrial orchids (Whigham et al 2006, Barsberg et al 2013. Very little is known about dormancy in epiphytic orchids, but Kartzinel et al (2013) found that 40 percent of the seeds desiccated or decayed within 5 months, and that habitat was the prime factor influencing recruitment.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Transient Population Dynamics of Two Epiphytic Orchid Species after Hurricane Ivan: Implications for Management

Raventós

González²,

Mújica

et al. 2015

Biotropica

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Populations of epiphytic orchids in disturbance‐prone environments rarely reach stable‐stage equilibrium. We characterized the post‐disturbance, transient dynamics of two epiphytic orchids, Broughtonia cubensis, and the leafless Dendrophylax lindenii, comparing the following indices: reactivity/first‐time attenuation, maximal amplification/attenuation, and amplified/attenuated inertia. We also assessed the effects of reintroducing only seeds or only adults, by examining the elasticity of the inertia on the vital rates. For 2006–2010, the stochastic growth rate of D. lindenii was λs = 0.94, or a 6 percent decrease per annum. First‐time step attenuation indicates that in 1 year, the population could decrease by an additional 16 percent, and in the worst‐case scenario could decrease by almost half, relative to the stable‐stage distribution, in 10 years. Broughtonia cubensis had a λs = 1.03; reactivity and first‐time step attenuation indicates that in 1 year, the population should not change by more than 14 percent of the estimated stable‐stage distribution. However, the worst‐case scenario projected a reduction of 40 percent relative to the stable‐stage distribution within 8 years. A comparison of reintroduction strategies assessed by elasticity of the population momentum showed that adults performed better when relocated to new habitats.

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“…, Barsberg et al . ). Very little is known about dormancy in epiphytic orchids, but Kartzinel et al .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Transient Population Dynamics of Two Epiphytic Orchid Species after Hurricane Ivan: Implications for Management

Raventós

González²,

Mújica

et al. 2015

Biotropica

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“…The samples stained with CBB and PAS demonstrate the accumulation of proteins and starch during the stages analyzed ( Figure 2L and 2M). These reserves were also observed using FTIR spectrophotometry (Figure 4), which has been previously used to analyze the composition of seeds (Kuhnen et al, 2010;Amir, Anjum, Khan, Khan, Pasha, & Nadeem, 2013;Barsberg, Rasmussen, & Kodahl, 2013;Araldi, Coelho, & Maraschin, 2016). Lipids, starch, proteins and phenolic compounds were present in all seed samples.…”

Section: Discussionmentioning

confidence: 99%

Anatomical and biochemical changes associated with the development and germination of <i>Araucaria angustifolia</i> seeds

Shibata

Coelho

Schmidt

et al. 2019

Acta Sci. Biol. Sci.

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This study aimed at assessing the cell cycle, and anatomical and biochemical changes that the embryonic axis of Araucaria angustifolia undergoes during development, focusing on the maturation stage. During all development, cells exhibited intense metabolic activity with an abundance of mitochondria, lipid bodies, and vacuolated cells. The continued accumulation of starch and protein was observed by LM and TEM and indicated by spectra of FTIR. Cell differentiation of the procambium was observed with a thickening of the cell wall and the formation of resiniferous ducts. At Stage III and IV, cells exhibited structural changes such as altered or elongated mitochondria and presence of plastoglobules. These results suggest that there is a gradual transition from developmental metabolism to germination metabolism. Such changes can contribute to the rapid germination of seeds right after their dispersion, making it an ecological strategy to reduce post-dispersal exposure to predators and to avoid damage from reduced moisture.

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“…), and it has also been suggested that ligninases in soil could facilitate seed germination by essentially scarifying the seed coat (Barsberg et al . ). Indeed, the evolution of ligninolytic peroxidases was one of the fundamental events driving the early co‐evolution of fungi and woody plants from the Permian onwards (Martin et al .…”

Section: Introductionmentioning

confidence: 97%

“…The location of lignin as a cell wall component in both inner and outer seed integuments is particularly well studied in a range of orchid species, and several contrasting methods not only confirm the presence of lignin but also provide a detailed picture of where and when lignin is synthesised, usually during the final stages of seed development resulting in ‘browning’ of the seeds (Yamazaki & Miyoshi ; Barsberg et al . ; Rao et al . ; Yang & Lee ).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic scarification of Anacamptis morio (Orchidaceae) seed facilitates lignin degradation, water uptake and germination

et al. 2018

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The seed coat of many species contains hydrophobic lignins, and in soil the action of microbial ligninases may contribute to release from dormancy. Laboratory use of ligninases to stimulate germination is promising because of the specific action on the seed coat, whereas chemical scarification agents may also corrode the embryo. We hypothesised that exposure of Anacamptis morio (Orchidaceae) seeds to fungal laccase would stimulate germination, and that the mechanism involves lignin degradation and increased imbibition. Germination capacity in vitro was quantified with 1 U filter-sterilised laccase added to agar medium following autoclaving, compared to a 10% bleach solution (standard bleach surface sterilisation/scarification method used in orchid seed sowing). Lignin degradation was quantified using an optical method (phloroglucinol-HCl staining) combined with image analysis, following experimental pre-treatments involving immersion in laccase solution, distilled water (negative control) or bleach (positive control). Water uptake after experimental treatments was quantified as the proportion of seeds exhibiting visible uptake of an aqueous fluorochrome under UV excitation. Laccase stimulated a doubling of germination in vitro with respect to bleach surface sterilisation/scarification alone, from 23.7 to 49.8% (P = 0.007). Laccase and bleach methods both significantly decreased the optical signal of phloroglucinol (for laccase, to 79.9 ± 1.3% of controls; anova: F = 10.333, P = 0.002). Laccase resulted in a modest but highly significant (P < 0.0001) increase in water uptake with respect to the control (11.7%; cf 99.4% for bleach). Laccase scarification can stimulate germination of A. morio through a mechanism of targeted seed coat degradation. The results demonstrate the potential of this relatively non-invasive enzymatic scarification technique.

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Composition of Cypripedium calceolus (Orchidaceae) seeds analyzed by attenuated total reflectance IR spectroscopy: In search of understanding longevity in the ground

Abstract: We suggest that degradation of lignin and enrichment of the testa surface with CaCO3 are important stimulants of germination both in the habitat and during laboratory sowing.

Cited by 24 publications

References 32 publications

Transient Population Dynamics of Two Epiphytic Orchid Species after Hurricane Ivan: Implications for Management

Transient Population Dynamics of Two Epiphytic Orchid Species after Hurricane Ivan: Implications for Management

Anatomical and biochemical changes associated with the development and germination of <i>Araucaria angustifolia</i> seeds

Enzymatic scarification of Anacamptis morio (Orchidaceae) seed facilitates lignin degradation, water uptake and germination

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