Composition of bacterial and archaeal communities in the rumen of dromedary camel using cDNA-amplicon sequencing

Rabee, Alaa Emara; Forster, Robert J.; Elekwachi, Chijioke O.; Kewan, Khaled Z.; Sabra, Ebrahim A.; Mahrous, Hoda; Khamiss, Omaima A.; Shawket, Safinaze M.

doi:10.1007/s10123-019-00093-1

Cited by 13 publications

(20 citation statements)

References 62 publications

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“…Firmicutes were dominated by the family Ruminococcaceae and Lachnospiraceae ( Tables 6 and 7 ), which is in agreement with results obtained by Rabee et al (2020a) . Members of family Ruminococcaceae degrade the hemicellulose, pectin, and cellulose present in the plant cell wall ( Pettipher & Latham, 1979 ), which could explain the high representation of this family in S1 group that fed mixture with high-NDF content and group S3 that fed mixture with high-ADF and NDF contents.…”

Section: Discussionsupporting

confidence: 90%

“…At the same time, no available data on the effect of discarded dates and date palm fronds on the rumen microbial populations. The higher ASVs were linked with sheep group fed S3 diet that contains concentrate mixture plus forage, which is similar to findings obtained by Petri et al (2012) and Rabee et al (2020a) . In this study, phylum Bacteroidetes and Firmicutes dominated the bacterial community; this finding was also reported in the rumen of lactating ewes fed olive cake ( Mannelli et al, 2018 ), cattle ( Petri et al, 2013 ), and camel ( Rabee et al, 2020a ).…”

Section: Discussionsupporting

confidence: 88%

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Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts

Rabee

Kewan

Sabra

et al. 2021

PeerJ

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Rumen bacteria make the greatest contribution to rumen fermentation that enables the host animal to utilize the ingested feeds. Agro-industrial byproducts (AIP) such as olive cake (OC) and date palm byproducts (discarded dates (DD), and date palm fronds (DPF)) represent a practical solution to the deficiency in common feed resources. In this study, thirty-six growing Barki lambs were divided into three groups to evaluate the effect of untraditional diets including the AIP on the growth performance. Subsequently, nine adult Barki rams were used to evaluate the effect of experimental diets on rumen fermentation and rumen bacteria. Three rations were used: common concentrate mixture (S1), common untraditional concentrate mixture including OC and DD (S2), and the same concentrate mixture in S2 supplemented with roughage as DPF enriched with 15% molasses (S3). The animals in S2 group showed higher dry matter intake (DMI) and lower relative growth rate (RGR) as compared to the animals in S1 group. However, the animals in S3 group were the lowest in DMI but achieved RGR by about 87.6% of that in the S1 group. Rumen pH, acetic and butyric acids were more prevalent in animals of S3 group and rumen ammonia (NH3-N), total volatile fatty acids (TVFA), propionic acid were higher in S1. Rumen enzymes activities were higher in S1 group followed by S3 and S2. The bacterial population was more prevalent in S1 and microbial diversity was higher in the S3 group. Principal coordinate analysis revealed clusters associated with diet type and the relative abundance of bacteria varied between sheep groups. The bacterial community was dominated by phylum Bacteroidetes and Firmicutes; whereas, Prevotella, Ruminococcus, and Butyrivibrio were the dominant genera. Results indicate that diet S3 supplemented by OC, DD, and DPF could replace the conventional feed mixture.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts

Rabee

Kewan

Sabra

et al. 2021

PeerJ

Self Cite

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show abstract

“…At the same time, no available data on the effect of discarded dates and date palm fronds on the rumen microbial populations. The higher ASVs were linked with sheep group fed S3 diet that contains concentrate mixture plus forage, which is similar to findings obtained by (Petri et al, 2012;Rabee et al, 2020a). In this study, phylum Bacteroidetes and Firmicutes dominated the bacterial community; this finding was also reported in the rumen of lactating ewes fed olive cake (Mannelli et al, 2018), cattle (Petri et al, 2013), and camel (Rabee et al, 2020a).…”

Section: Diversity and Composition Of Rumen Bacteriasupporting

confidence: 88%

Peer Review #1 of "Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts (v0.1)"

García‐Contreras¹

2021

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“…The structure of ALNA is highly similar to ileal PPs. In this study, the common dominant bacteria colonized the mucosa surface of the ALNA and ileal PPs were the same as in the intestine of other animals, such as dromedary [20], Bactrian camel [19], cattle [32], sheep [33] and horse [34]. There are several reasons for this phenomenon: first, the similar microenvironment of the intestine, such as the same temperature (maintained at about 37-40˚C) in vivo [35], which provided the foundation for the stable reproduction of microorganisms; second, the similar chyme components, for the herbivores, including large amounts of plant cellulose, fructose, starch granules, etc.…”

Section: The Relationship Between the Host And Common Dominant Bacteriamentioning

confidence: 65%

“…At present, the studies on the bacterial community in the gastrointestinal tract of camels mainly focused on rumen, intestines, and/or feces, and the results suggested that the composition of microbial community in the rumen of a camel was similar to other ruminants with differences in the abundance, meanwhile, there were different metagenomic profiles in different digestive tract regions [19,20]. However, the studies about the different mucosal immune inductive sites of gastrointestinal tract has only received few attentions.…”

Section: Introductionmentioning

confidence: 99%

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

Zhang

Yao

et al. 2020

PLoS ONE

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The microbial communities colonize the mucosal immune inductive sites could be captured by hosts, which could initiate the mucosal immune responses. The aggregated lymphoid nodule area (ALNA) and the ileal Payer's patches (PPs) in Bactrian camels are both the mucosal immune inductive sites of the gastrointestinal tract. Here, the bacteria community associated with the ALNA and ileal PPs were analyzed using of 16S rDNA-Illumina Miseq sequencing. The mutual dominant bacterial phyla at the two sites were the Bacteroidetes, Firmicutes, Verrucomicrobia and Proteobacteria, and the mutual dominant genus in both sits was Prevotella. The abundances of the Fibrobacter, Campylobacter and RFP12 were all higher in ALNA than in ileal PPs. While, the abundances of the 5-7N15, Clostridium, and Escherichia were all higher in ileal PPs than in ALNA. The results suggested that the host's intestinal microenvironment is selective for the symbiotic bacteria colonizing the corresponding sites, on the contrary, the symbiotic bacteria could impact on the physiological functions of this local site. In ALNA and ileal PPs of Bactrian camel, the bacteria which colonized different immune inductive sites have the potential to stimulate different immune responses, which is the result of the mutual selection and adaptation between microbial communities and their host.

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Composition of bacterial and archaeal communities in the rumen of dromedary camel using cDNA-amplicon sequencing

Cited by 13 publications

References 62 publications

Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts

Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts

Peer Review #1 of "Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts (v0.1)"

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

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