Composition effects on photooxidative membrane destabilization by TiO2 nanoparticles

Häffner, Sara Malekkhaiat; Parra-Ortiz, Elisa; Skoda, Maximilian W. A.; Saerbeck, Thomas; Browning, Kathryn L.; Malmsten, Martin

doi:10.1016/j.jcis.2020.09.046

Cited by 24 publications

(36 citation statements)

References 77 publications

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“…S7 and S8†), comparable to those previously observed by us for UV exposure alone. 16,17 Therefore, the effect of the silica surface topography in the absence of TiO 2 did not show a significant effect on top of the UV effect.…”

Section: Resultsmentioning

confidence: 89%

“…While cholesterol was found to provide stabilization against TiO 2 -triggered oxidation of PC-based bilayers, the presence of anionic PG made these bilayers more sensitive to oxidative destabilization, likely an effect of less dense packing, and/or the occurrence of different free-radical processes. 17 Neutron reflectometry and small-angle X-ray scattering furthermore showed that lipid oxidation caused by UV illumination of TiO 2 nanoparticles triggered pronounced structural changes of the bilayers, including increased hydration, lipid removal, bilayer thinning, lateral phase separation, and aggregate solubilization. 16,17 In comparison, UV exposure in presence of H 2 O 2 but in the absence of TiO 2 nanoparticles resulted in quantitatively smaller effects.…”

Section: Discussionmentioning

confidence: 99%

“…17 Neutron reflectometry and small-angle X-ray scattering furthermore showed that lipid oxidation caused by UV illumination of TiO 2 nanoparticles triggered pronounced structural changes of the bilayers, including increased hydration, lipid removal, bilayer thinning, lateral phase separation, and aggregate solubilization. 16,17 In comparison, UV exposure in presence of H 2 O 2 but in the absence of TiO 2 nanoparticles resulted in quantitatively smaller effects. 39 Extending on these previous studies on free TiO 2 nanoparticles, the present study shows that qualitatively similar effects are obtained for TiO 2 nanoparticles incorporated into larger matrix particles, such as mesoporous silica nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we investigated effects of UV-activated TiO 2 nanoparticles on different lipid membranes, including model mammalian cell (phosphatidylcholine (PC)/cholesterol) and bacterial (PC/phosphatidylglycerol (PG)) membranes. 16,17 It was observed that incorporation of anionic PG dramatically enhanced the susceptibility of these membranes to oxidative degradation, whereas cholesterol was found to have a stabilizing affect against oxidation. 17 Extending on this previous work, we set out in the present study to investigate the interaction of combined SiO 2 /TiO 2 nanoparticles with previously employed PC/PG-based model lipid system, investigating supported lipid bilayers and large unilamellar vesicles using a set of complementary techniques, including neutron reflectometry (NR), cryogenic transition electron microscopy (cryo-TEM), fluorescence oxidation and leakage assays, dynamic light scattering (DLS), and ζ-potential measurements, for a comprehensive investigation of membrane interactions.…”

Section: Introductionmentioning

confidence: 99%

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Mesoporous silica as a matrix for photocatalytic titanium dioxide nanoparticles: lipid membrane interactions

et al. 2022

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Section: Resultsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mesoporous silica as a matrix for photocatalytic titanium dioxide nanoparticles: lipid membrane interactions

et al. 2022

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“…With acid pH, the nanoparticles have a positive charge that allowed the interaction with the membrane cell. In addition, UV irradiation has a greater effect on membrane stabilization, triggering oxidative stress [ 229 ].…”

Section: Nanotechnology Applied To Antimicrobial Resistancementioning

confidence: 99%

Antimicrobial Resistance and Inorganic Nanoparticles

Balderrama-González

Piñón-Castillo

Ramírez-Valdespino

et al. 2021

IJMS

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Antibiotics are being less effective, which leads to high mortality in patients with infections and a high cost for the recovery of health, and the projections that are had for the future are not very encouraging which has led to consider antimicrobial resistance as a global health problem and to be the object of study by researchers. Although resistance to antibiotics occurs naturally, its appearance and spread have been increasing rapidly due to the inappropriate use of antibiotics in recent decades. A bacterium becomes resistant due to the transfer of genes encoding antibiotic resistance. Bacteria constantly mutate; therefore, their defense mechanisms mutate, as well. Nanotechnology plays a key role in antimicrobial resistance due to materials modified at the nanometer scale, allowing large numbers of molecules to assemble to have a dynamic interface. These nanomaterials act as carriers, and their design is mainly focused on introducing the temporal and spatial release of the payload of antibiotics. In addition, they generate new antimicrobial modalities for the bacteria, which are not capable of protecting themselves. So, nanoparticles are an adjunct mechanism to improve drug potency by reducing overall antibiotic exposure. These nanostructures can overcome cell barriers and deliver antibiotics to the cytoplasm to inhibit bacteria. This work aims to give a general vision between the antibiotics, the nanoparticles used as carriers, bacteria resistance, and the possible mechanisms that occur between them.

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Antimicrobial Peptide Coating of TiO₂ Nanoparticles for Boosted Antimicrobial Effects

Caselli,

Traini,

Micciulla

et al. 2024

Adv Funct Materials

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This study explores the coating of photocatalytic nanoparticles with antimicrobial peptides (AMPs) for boosted antimicrobial effects, and how such effects depend on AMP properties. For this, TiO2 nanoparticles are coated with the AMP KYE21 or its hydrophobically enhanced variant WWWKYE21. Mirroring effects of free peptides, coated nanoparticles displayed higher binding and UV‐induced degradation for bacteria‐like than for mammalian‐like membranes. In addition, they degraded bacterial lipopolysaccharides (LPS). WWWKYE21‐coated nanoparticles displayed higher binding to LPS and bacteria‐like membranes and photocatalytic degradation, although saturation effects are found at high nanoparticle binding. Neutron reflectometry showed that binding of peptide‐coated nanoparticles to bacteria‐like membranes resulted in partial lipid removal in the absence of UV, but that UV illumination caused additional degradation, featuring increases in the hydration of headgroup and acyl chain regions. For LPS, UV induced removal of its outer O‐antigen region. Analogous to findings in model systems, antimicrobial effects of peptide‐coated nanoparticles against Escherichia coli bacteria on illumination are pronounced, while toxicity against human monocytes remained low. Altogether, results show that AMP coating boosts the antimicrobial effects of photocatalytic nanoparticles without causing cell toxicity. From a broader perspective, the study points to the potential of nanoarchitectonic combination of component properties for the design of composite NP properties.

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Composition effects on photooxidative membrane destabilization by TiO2 nanoparticles

Cited by 24 publications

References 77 publications

Mesoporous silica as a matrix for photocatalytic titanium dioxide nanoparticles: lipid membrane interactions

Mesoporous silica as a matrix for photocatalytic titanium dioxide nanoparticles: lipid membrane interactions

Antimicrobial Resistance and Inorganic Nanoparticles

Antimicrobial Peptide Coating of TiO₂ Nanoparticles for Boosted Antimicrobial Effects

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Composition effects on photooxidative membrane destabilization by TiO2 nanoparticles

Cited by 24 publications

References 77 publications

Mesoporous silica as a matrix for photocatalytic titanium dioxide nanoparticles: lipid membrane interactions

Mesoporous silica as a matrix for photocatalytic titanium dioxide nanoparticles: lipid membrane interactions

Antimicrobial Resistance and Inorganic Nanoparticles

Antimicrobial Peptide Coating of TiO2 Nanoparticles for Boosted Antimicrobial Effects

Contact Info

Product

Resources

About

Antimicrobial Peptide Coating of TiO₂ Nanoparticles for Boosted Antimicrobial Effects