Composition and Structure of Lipopolysaccharides from Pseudomonas aeruginosa

Wilkinson, Stephen G.

doi:10.1093/clinids/5.supplement_5.s941

Cited by 109 publications

(101 citation statements)

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“…This doublet probably represents substoichiometric modification in the core or lipid A of the B-band components. It has been reported for different gram-negative bacteria that there is microheterogeneity in the structure of lipid A (phosphate levels and types and numbers of fatty acids) (2,51,57), in the substituents in the core (14,22,(36)(37)(38)57), and in modifications of the 0-antigen side chain (36,37,57). The heterogeneity of LPS molecules presumably depends in part on the strain and on growth conditions (13,36).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that S. paratyphi B and S. typhimurium can synthesize a Ti polysaccharide and an 0 polysaccharide attached to the same core (37,41) and that the synthesis of the two molecules is independent (37). Other P. aeruginosa strains may also be able to synthesize more than one type of LPS with different 0-antigenic side chains (8,30,57,58). The highmolecular-weight polysaccharide released from LPS of these strains has been resolved into amino sugar-rich and neutral sugar-rich fractions when separated by gel permeation chromatography (30, 57, 58).…”

Section: Discussionmentioning

confidence: 99%

“…Structural microheterogeneity in several regions of LPS molecules from members of the family Enterobacteriaceae (2,14,22,37,38,48,51) and Pseudomonas aeruginosa strains (33,57) has been demonstrated. Of the several methods used to separate the subclasses of LPS from individual strains, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (20,24,45,48) and gel filtration (9,26,30,32,34,48) are the best.…”

mentioning

confidence: 98%

“…Compositional analysis of LPS from P. aeruginosa has indicated that the LPS molecules are structurally similar to enterobacterial LPS molecules, but possess several distinctive features (33,57). The most outstanding differences include the unusually high phosphate content (33,58), the presence of L-alanine in the core (33,57), and the high levels of amino sugars and uronic acids in the 0 side chain (8,33).…”

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confidence: 99%

“…The most outstanding differences include the unusually high phosphate content (33,58), the presence of L-alanine in the core (33,57), and the high levels of amino sugars and uronic acids in the 0 side chain (8,33). The characterization of P. aeruginosa 0-specific polysac-* Corresponding author.…”

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Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length

et al. 1988

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Lipopolysaccharide (LPS) from smooth strains of Pseudomonas aeruginosa 503, PAZ1, PA01715, PA01716, and Z61 was fractionated by gel filtration chromatography. LPS samples from the first four strains, all PAO1 derivatives, separated into three major size populations, whereas LPS from strain Z61, a Pac K799/WT mutant strain, separated into two size populations. When column fractions were applied to sodium dodecyl sulfate-polyacrylamide gels in their order of elution, molecules of decreasing size were resolved, and the ladder of molecules with different-length 0 antigens formed a diagonal across the gel. The LPS from the PAO1 derivatives contained two distinct sets of bands, distinguished on the gels as two sets of diagonals. The set of bands with the faster mobility, the B bands, was found in column fractions comprising the three major amino sugar-containing peaks. In the sample from strain 503, a fourth minor peak which contained B bands was resolved. The slower-moving set of bands, the A bands, were recovered in a minor peak. LPS from strain Z61 contained only one set of bands, with the higher-molecular-weight molecules eluting from the column in a volume similar to that of the B bands of the PAO1 strains. Analysis of the fractions of LPS from all strains indicated that less than 8% of the LPS molecules had a long, attached 0 antigen. Analysis of the peak that contained mainly A bands indicated a lack of reactive amino sugar and phosphate, although heptose and 2-keto-3-deoxyoctulosonic acid were detected. Reaction of isolated fractions with monoclonal antibody specific for the PA01 0-antigen side chain indicated that only the B bands from the PAO1 strains were antigenically reactive. The bands from strain Z61 showed no reactivity. The data suggest that the A and B bands from the PAO1 strains are antigenically distinct. We propose that PAO1 strains synthesize two types of molecules that are antigenically different.Lipopolysaccharide (LPS), a major component of the outer membranes of gram-negative bacteria, is important in the structure (33, 37) and function (33,36) of this membrane. Structural microheterogeneity in several regions of LPS molecules from members of the family Enterobacteriaceae (2,14,22,37,38,48,51) and Pseudomonas aeruginosa strains (33, 57) has been demonstrated. Of the several methods used to separate the subclasses of LPS from individual strains, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (20,24,45,48) and gel filtration (9, 26, 30, 32, 34, 48) are the best. Either of these two methods by themselves, however, may be insufficient to completely characterize the high-and low-molecular-weight fractions of LPS. Peterson and McGroarty (48) demonstrated that the SDS-PAGE of the column fractions of samples from Salmonella typhimurium, Salmonella minnesota, and Escherichia coli was instrumental in characterizing the various-sized fractions. Analysis of the isolated fractions allowed for the estimation of the average number of 0-antigen repeat units per LPS from each of the si...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length

et al. 1988

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Pseudomonas

Palleroni

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Pseu.do' mo.nas or Pseu.do.mo' nas . Gr. adj. pseudes false; Gr. n. monas a unit, monad; M.L. fem. n. Pseudomonas false monad. Proteobacteria / Gammaproteobacteria / Pseudomonadales / Pseudomonadaceae / Pseudomonas Straight or slightly curved rods but not helical, 0.5–1.0 × 1.5–5.0 µm. Most of the species do not accumulate granules of polyhydroxybutyrate , but accumulation of polyhydroxyalkanoates of monomer lengths higher than C 4 may occur when growing on alkanes or gluconate. Do not produce prosthecae and are not surrounded by sheaths. No resting stages are known. Gram negative. Motile by one or several polar flagella ; rarely nonmotile. In some species lateral flagella of short wavelength may also be formed. Aerobic, having a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor; in some cases nitrate can be used as an alternate electron acceptor , allowing growth to occur anaerobically. Xanthomonadins are not produced . Most, if not all, species fail to grow under acid conditions (pH 4.5 or lower). Most species do not require organic growth factors. Oxidase positive or negative. Catalase positive. Chemoorganotrophic. Strains of the species include in their composition the hydroxylated fatty acids C 10 : 0 3OH and C 12 : 0 , and C 12 : 0 2OH , and ubiquinone Q‐9 . Widely distributed in nature. Some species are pathogenic for humans, animals, or plants. The mol % G + C of the DNA is : 58–69. Type species : Pseudomonas aeruginosa (Schroeter 1872) Migula 1900, 884 ( Bacterium aeruginosum Schroeter 1872, 126.)

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Enhancement of sample loadings for the analysis of oligosaccharides isolated from Pseudomonas aeruginosa using transient isotachophoresis and capillary zone electrophoresis—electrospray—mass spectrometry

et al. 1998

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The analysis of underivatized core oligosaccharides arising from mild acid hydrolysis of lipopolysaccharides from Pseudomonas aeruginosa serotype 05 was achieved using a transient isotachophoretic preconcentration method coupled to capillary zone electrophoresis-electrospray-mass spectrometry (tCITP-CZE-ES-MS). The combination of a tCITP preconcentration step provided a 10- to 50-fold enhancement of sample loading and a corresponding improvement in sensitivity compared to the conventional zone electrophoresis format. Electrophoretic conditions, enabling the separation of these anionic analytes, were developed to determine possible sites of heterogeneity on either the core or the O-chain structures. The tCITP-CZE-ES-MS technique provided unparalleled resolution of the different core glycoforms and oligosaccharides obtained from the acid cleavage of the native endotoxins whether isolated following conventional gel permeation chromatography or obtained from direct hydrolysis of the bacterial isolates. These investigations also highlighted the highly phosphorylated nature of these complex cell membrane components, where the heptose residues of the core oligosaccharide can bear up to six phosphate groups.

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Composition and Structure of Lipopolysaccharides from Pseudomonas aeruginosa

Cited by 109 publications

References 43 publications

Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length

Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length

Pseudomonas

Enhancement of sample loadings for the analysis of oligosaccharides isolated from Pseudomonas aeruginosa using transient isotachophoresis and capillary zone electrophoresis—electrospray—mass spectrometry

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