Composition and characterization of in situ usable light cured dental drug delivery hydrogel system

Abstract

Biodegradable polymers are compatible, permeable and nontoxic, thus they can provide a useful tool for drug delivery or tissue engineering. These polymers can form hydrogels, which are suitable vehicles for different types of materials e.g. drugs, bioactive molecules or cells. In the case of dentistry, photopolymerization is an obvious method to obtain in situ useable devices which can provide a more efficient way of tailoring drug release. A hydrogel system was developed based on poly-gamma-glutamic acid that was modified with methacryloyl groups to achieve this purpose. The resulting new reactive structure was proved by NMR spectroscopy. The swelling ratio of this type of hydrogel has been found remarkable, over 300 % after 24 h, and it can release 5 ng/mm(2) metronidazole. The prepared hydrogels were nontoxic as viability, cytotoxicity tests and cell morphology investigations proved it. These results render this model system an excellent candidate for use as an in situ curing local drug delivery device. The new photoactive system can be utilized in the treatment of periodontal diseases or raising the effectiveness of drugs used only in the minimal effective dose.

Preparation and characterization of Poly(γ-glutamic acid) hydrogels as potential tissue engineering scaffolds

“…Condensation of -PGA was always followed by side reactions. Compared with the above crosslinking methods, photo-crosslinking [20,21] , which does not involve harsh conditions such as high energy irradiation or toxic chemicals, is much more cytocompatible and can achieve better spatiotemporal control over the polymerization process and form more homogenous network structure [22] .…”

Poly-γ-Glutamic Acid Nanoparticles Based Visible Light-Curable Hydrogel for Biomedical Application

“…The final result was expressed by the percentage of the original drug content. In the cases of mechanical analysis and determinations of swelling and release properties the results of PGA nanogels were compared with methacrylated-PGA hydrogels (MPGA hydrogels) [29]. The schematic illustration of the hydrogel and nanogel creation is shown in Figure 2.…”

“…9 * 10 4 cells/well were placed in a 24-well cell culture plate and cells were let to attach to the bottom of the wells for 4 hours. Attached cells were washed with colorless DMEM (Sigma Aldrich, D5921) and were incubated at 37 ∘ C in a CO 2 incubator for 2 hours in Alamar Blue reagent diluted Figure 2: Schematic illustration of the creation of the earlier described MPGA hydrogel [29], and the PGA nanogel.…”

“…The lower energy cannot cause any harmful effects, and this advantage is important in biomaterials. In our previous study a -PGA-based photopolymerizable hydrogel was developed, and it was described as an alternative drug delivery vehicle [29]. Recognizing the new possibilities of nanoparticles we enhanced the original concept.…”

Ultrafast in situ forming poly(ethylene glycol)-poly(amido amine) hydrogels with tunable drug release properties via controllable degradation rates

“…Compared to injectable physically crosslinked hydrogels, chemically crosslinked hydrogels usually have better mechanical properties and are more resistant to degradation and dissolution. Injectable chemically crosslinked hydrogels have been mostly prepared by photocrosslinking of (meth)acrylate functionalized polymers using UV light [5,6] or by covalent crosslinking between polymers with complementary functional groups [7,8].…”