Poly-γ-Glutamic Acid Nanoparticles Based Visible Light-Curable Hydrogel for Biomedical Application

Abstract

Nanoparticles and hydrogels have gained notable attention as promising potential for fabrication of scaffolds and delivering materials. Visible light-curable systems can allow for the possibility ofin situfabrication and have the advantage of optimal applicability. In this study nanogel was created from methacrylated poly-gamma-glutamic acid nanoparticles by visible (dental blue) light photopolymerization. The average size of the particles was 80 nm by DLS, and the NMR spectra showed that the methacrylation rate was 10%. Polymerization time was 3 minutes, and a stable nanogel with a swelling rate of 110% was formed. The mechanical parameters of the prepared structure (compression stress 0.73 MPa, and Young’s modulus 0.93 MPa) can be as strong as necessary in a real situation, for example, in the mouth. A retaining effect of the nanogel was found for ampicillin, and the biocompatibility of this system was tested by Alamar Blue proliferation assay, while the cell morphology was examined by fluorescence and laser scanning confocal microscopy. In conclusion, the nanogel can be used for drug delivery, or it can be suitable for a control factor in different systems.

Honey Mediated Green Synthesis of Nanoparticles: New Era of Safe Nanotechnology

“…Over the past decade, nanomaterials emerged as promising commodities in many fields including cosmetics, healthcare, biomedical, food and feed, drug-gene delivery, environment, health, mechanics, optics, chemical industries, electronics, space industries, energy science, catalysis, light emitters, single electron transistors, nonlinear optical devices, and photoelectrochemical applications [1][2][3][4][5][6][7][8][9][10].…”

Improved Exopolymer Production by Chromohalobacter canadensis Cultures for Its Potential Cosmeceutical Applications

“…Poly-gamma-glutamic acid (γ-PGA) is a naturally occurring, biodegradable, edible, nontoxic, environmentally friendly, and nonimmunogenic poly-amino acid [20]. Several bacteria like Bacillus subtilis (natto), B. licheniformis, B. amyloliquefaciens, B. pumilis, B. megaterium, B. mojavensis, B. anthracis [21], and halophilic archea Natrialba aegyptiaca [22] produce this compound.…”

Design and Applications of Tumor Microenvironment-Responsive Nanogels as Drug Carriers

“…Therefore, visible light-induced photopolymerization has been developed because they have longer wavelengths, less scattered by larger objects and better penetration (Matsui et al, 2017;Le Quemener et al, 2018). For example, Bakó prepared nanogels using methacrylic acid poly-γ-glutamic nanoparticles loading antibiotic drug ampicillin by visible (blue) light-initiated photopolymerization, and the release kinetics showed the controlled and efficient release behaviors (Bakó et al, 2016). Notably, our lab has also developed some strategies on the preparation of nanogels through laser photopolymerization, which is related with the investigation of the initiating system, polymerization mechanism and biomedical applications (Li J. et al, 2021;Liu et al, 2021;Peng et al, 2021;Wang et al, 2021).…”