Composite P(3HB-3HV)-CS Spheres for Enhanced Antibiotic Efficiency

Abstract

Natural-derived biopolymers are suitable candidates for developing specific and selective performance-enhanced antimicrobial formulations. Composite polymeric particles based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and chitosan, P(3HB-3HV)-CS, are herein proposed as biocompatible and biodegradable delivery systems for bioproduced antibiotics: bacitracin (Bac), neomycin (Neo) and kanamycin (Kan). The stimuli-responsive spheres proved efficient platforms for boosting the antibiotic efficiency and antibacterial susceptibility, as evidenced against Gram-positive and Gram-negative strains. Absent or reduced proinflammatory effects were evidenced on macrophages in the case of Bac-/Neo- and Kan-loaded spheres, respectively. Moreover, these systems showed superior ability to sustain and sustain the proliferation of dermal fibroblasts, as well as to preserve their ultrastructure (membrane and cytoskeleton integrity) and to exhibit anti-oxidant activity. The antibiotic-loaded P(3HB-3HV)-CS spheres proved efficient alternatives for antibacterial strategies.

Bioactive Coatings Loaded with Osteogenic Protein for Metallic Implants

“…Special attention was oriented towards the latter category, given the enhanced physicochemical properties and boosted biofunctional performance of laser-textured [22][23][24] and coated [25,26] metallic implants. Out of all these techniques, the matrix-assisted pulsed laser evaporation (MAPLE) is known to have some advantages, such as the possibility to synthesize homogeneous and uniform nanosized or nanostructured coatings, as well as composite or hybrid coatings containing organic substances, such as polymers [27][28][29] and biomolecules [30,31].…”