Composite alginate-gelatin hydrogels incorporating PRGF enhance human dental pulp cell adhesion, chemotaxis and proliferation

Anitua, Eduardo; Zalduendo, Mar; Troya, María; Erezuma, Itsasne; Lukin, Izeia; Hernáez‐Moya, Raquel; Orive, Gorka

doi:10.1016/j.ijpharm.2022.121631

Cited by 11 publications

(4 citation statements)

References 52 publications

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“…The first 0.5 ml upper of the leucocytes’ layer is collected as PRGF; the rest was nominated as plasma poor in growth factors (PPGF). Subsequently, to activate PRGF, 50 µl of 10% calcium chloride (CaCl 2 MERK) was poured into PRGF, and after 15 min, the final product was achieved [ 57 – 61 ].…”

Section: Methodsmentioning

confidence: 99%

Preparation and In Vitro Osteogenic Evaluation of Biomimetic Hybrid Nanocomposite Scaffolds Based on Gelatin/Plasma Rich in Growth Factors (PRGF) and Lithium-Doped 45s5 Bioactive Glass Nanoparticles

et al. 2022

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Bone tissue engineering is an emerging technique for repairing large bone lesions. Biomimetic techniques expand the use of organic–inorganic spongy-like nanocomposite scaffolds and platelet concentrates. In this study, a biomimetic nanocomposite scaffold was prepared using lithium-doped bioactive-glass nanoparticles and gelatin/PRGF. First, sol–gel method was used to prepare bioactive-glass nanoparticles that contain 0, 1, 3, and 5%wt lithium. The lithium content was then optimized based on antibacterial and MTT testing. By freeze-drying, hybrid scaffolds comprising 5, 10, and 20% bioglass were made. On the scaffolds, human endometrial stem cells (hEnSCs) were cultured for adhesion (SEM), survival, and osteogenic differentiation. Alkaline phosphatase activity and osteopontin, osteocalcin, and Runx2 gene expression were measured. The effect of bioactive-glass nanoparticles and PRGF on nanocomposites' mechanical characteristics and glass-transition temperature ( T g ) was also studied. An optimal lithium content in bioactive glass structure was found to be 3% wt. Nanoparticle SEM examination indicated grain deformation due to different sizes of lithium and sodium ions. Results showed up to 10% wt bioactive-glass and PRGF increased survival and cell adhesion. Also, Hybrid scaffolds revealed higher ALP-activity and OP, OC, and Runx2 gene expression. Furthermore, bioactive-glass has mainly increased ALP-activity and Runx2 expression, whereas PRGF increases the expression of OP and OC genes. Bioactive-glass increases scaffold modulus and T g continuously. Hence, the presence of both bioactive-glass and nanocomposite scaffold improves the expression of osteogenic differentiation biomarkers. Subsequently, it seems that hybrid scaffolds based on biopolymers, Li-doped bioactive-glass, and platelet extracts can be a good strategy for bone repair. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Preparation and In Vitro Osteogenic Evaluation of Biomimetic Hybrid Nanocomposite Scaffolds Based on Gelatin/Plasma Rich in Growth Factors (PRGF) and Lithium-Doped 45s5 Bioactive Glass Nanoparticles

et al. 2022

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“…The hydroxyapatite‐contained GAHA and GAHAP hydrogels showed higher mechanical strength, proliferative rate, and osteogenic differentiation, while Plasma Rich in Growth Factor significantly enhanced cell adhesion and chemotaxis. [ 42 ]…”

Section: High‐swelling Hydrogelsmentioning

confidence: 99%

Tailoring the Swelling‐Shrinkable Behavior of Hydrogels for Biomedical Applications

Feng

Wang

2023

Advanced Science

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Hydrogels with tailor‐made swelling‐shrinkable properties have aroused considerable interest in numerous biomedical domains. For example, as swelling is a key issue for blood and wound extrudates absorption, the transference of nutrients and metabolites, as well as drug diffusion and release, hydrogels with high swelling capacity have been widely applicated in full‐thickness skin wound healing and tissue regeneration, and drug delivery. Nevertheless, in the fields of tissue adhesives and internal soft‐tissue wound healing, and bioelectronics, non‐swelling hydrogels play very important functions owing to their stable macroscopic dimension and physical performance in physiological environment. Moreover, the negative swelling behavior (i.e., shrinkage) of hydrogels can be exploited to drive noninvasive wound closure, and achieve resolution enhancement of hydrogel scaffolds. In addition, it can help push out the entrapped drugs, thus promote drug release. However, there still has not been a general review of the constructions and biomedical applications of hydrogels from the viewpoint of swelling‐shrinkable properties. Therefore, this review summarizes the tactics employed so far in tailoring the swelling‐shrinkable properties of hydrogels and their biomedical applications. And a relatively comprehensive understanding of the current progress and future challenge of the hydrogels with different swelling‐shrinkable features is provided for potential clinical translations.

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“…The printable hydrogel, in comparison to empty scaffolds, also prolonged degradation time and demonstrated its ability to promote cell adhesion, proliferation and differentiation in vitro as well as to regenerate bone tissue in vivo by attracting endogenous stem cells and creating vascular constructs [108]. Gelatin-derived ink also permits the integration of patient-derived stimulating compounds such as platelet-rich plasma or platelet-rich growth factors [109,111]. The addition of stimulating agents to bioinks may accelerate the regeneration process, since it provokes growths factor release and thus attracts more cells.…”

Section: Gelatin As Bioink For 3d Printingmentioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

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Composite alginate-gelatin hydrogels incorporating PRGF enhance human dental pulp cell adhesion, chemotaxis and proliferation

Cited by 11 publications

References 52 publications

Preparation and In Vitro Osteogenic Evaluation of Biomimetic Hybrid Nanocomposite Scaffolds Based on Gelatin/Plasma Rich in Growth Factors (PRGF) and Lithium-Doped 45s5 Bioactive Glass Nanoparticles

Preparation and In Vitro Osteogenic Evaluation of Biomimetic Hybrid Nanocomposite Scaffolds Based on Gelatin/Plasma Rich in Growth Factors (PRGF) and Lithium-Doped 45s5 Bioactive Glass Nanoparticles

Tailoring the Swelling‐Shrinkable Behavior of Hydrogels for Biomedical Applications

Progress in Gelatin as Biomaterial for Tissue Engineering

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