Component-resolved diagnostics: laboratory results are not enough

Plebani, Mario

doi:10.1515/cclm-2013-0367

Cited by 2 publications

(1 citation statement)

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“…Critical approach to the application of this method implies a good co-operation between the specialist in medical biochemistry and laboratory medicine and the specialist allergologist, and the common consistency in the interpretation of the obtained results ( 63 , 64 ). The final interpretation is influenced by the pre-analytical, analytical and post-analytical phase of the CRD.…”

Section: Necessity For Careful Interpretation Of Crd Resultsmentioning

confidence: 99%

The potential of component-resolved diagnosis in laboratory diagnostics of allergy

Dodig¹,

Čepelak

2018

Biochemia Medica

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The initial laboratory approach in the diagnosis of allergies is to detect the type of allergic reaction, i.e. whether the patient’s allergy is mediated by immunoglobulin E (IgE) or not. For this purpose, the concentration of total serum IgE (tIgE) and specific IgE (sIgE) are determined. Progress in laboratory diagnostics is the use of component-resolved diagnosis (CRD) which implies determination of sIgE against purified native and recombinant allergenic molecules. Component-resolved diagnosis is used in laboratory practice as singleplex and multiplex assays. The choice of allergen for singleplex assay is based on anamnesis, clinical findings of a patient and on skin prick test results. Multiplex-microarray assays simultaneously determine multiple sIgE’s against numerous allergens. The goal of CRD is to distinguish the true allergens from the cross-reactive allergen molecules. Component-resolved diagnosis allows predicting the risk of severe symptoms, as well as anticipating the development of allergies. Thus, determination of sIgE against allergenic components may significantly improve current diagnostics of allergy. Since this method is applied in laboratory practice just a few years, it is necessary to acquire new knowledge and experience, to establish good co-operation between specialist in medical biochemistry and laboratory medicine and the specialist allergologist, so that the method can be applied in a rational manner. Component-resolved diagnosis will significantly improve the diagnostics of IgE-mediated allergy in the future. The aim of this article is to present potentials of CRD in the laboratory diagnostics of allergy mediated by IgE.

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Section: Necessity For Careful Interpretation Of Crd Resultsmentioning

confidence: 99%